Human Dentin Production in Vitro

About, Imad; Bottero, Marie-José; Denato, Philippe de; Camps, Jean; Franquin, Jean‐Claude; Mitsiadis, Thimios A.

doi:10.1006/excr.2000.4909

Cited by 248 publications

(219 citation statements)

References 45 publications

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“…After a dental injury, dental pulp is involved in a process called reparative dentinogenesis, where cells elaborate and deposit a new dentin matrix for the repair of the injured site (Mitsiadis and Rahiotis, 2004). It has been shown that adult dental pulp contains precursors capable of forming odontoblasts under appropriate signals (About et al, 2000;About and Mitsiadis, 2001;Alliot-Licht et al, 2005;Gronthos et al, 2000Gronthos et al, , 2002Miura et al, 2003;Tecles et al, 2005). Among these signals are the calcium hydroxide or calcium phosphate materials, which constitute pulp-capping materials used by dentists for common dental treatments.…”

Section: Stem Cells In Regenerative Medicinementioning

confidence: 99%

Stem cells for tooth engineering

Bluteau

Luder

Bari³

et al. 2008

eCM

167

152

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Tooth development results from sequential and reciprocal interactions between the oral epithelium and the underlying neural crest-derived mesenchyme. The generation of dental structures and/or entire teeth in the laboratory depends upon the manipulation of stem cells and requires a synergy of all cellular and molecular events that finally lead to the formation of tooth-specific hard tissues, dentin and enamel. Although mesenchymal stem cells from different origins have been extensively studied in their capacity to form dentin in vitro, information is not yet available concerning the use of epithelial stem cells. The odontogenic potential resides in the oral epithelium and thus epithelial stem cells are necessary for both the initiation of tooth formation and enamel matrix production. This review focuses on the different sources of stem cells that have been used for making teeth in vitro and their relative efficiency. Embryonic, post-natal or even adult stem cells were assessed and proved to possess an enormous regenerative potential, but their application in dental practice is still problematic and limited due to various parameters that are not yet under control such as the high risk of rejection, cell behaviour, long tooth eruption period, appropriate crown morphology and suitable colour. Nevertheless, the development of biological approaches for dental reconstruction using stem cells is promising and remains one of the greatest challenges in the dental field for the years to come.

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Section: Stem Cells In Regenerative Medicinementioning

confidence: 99%

Stem cells for tooth engineering

Bluteau

Luder

Bari³

et al. 2008

eCM

167

152

View full text Add to dashboard Cite

show abstract

“…19,20 Easy obtained potential with minimum pain & morbidity make human DPSCs as a valuable source of MSCs compared to the ordinary sources, such as bone marrow mesenchymal stem cells 21 . In general, DPSCs have been isolated by either outgrowth method, i.e., migration of stem cells from pulp tissue explants (DPSC-OG) [22][23][24] , and/or by enzymatic digestion (DPSC-ED) 4,6,25 . Previous studies have shown that the isolation method and culture conditions can induce different populations or lineages under in vitro passages 26,27 .…”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Karamzadeh

Eslaminejad

Aflatoonian

2012

JoVE

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“…In this context, culture of pulp cells appears as a promising tool to ensure the dental tissue regeneration (Bohl et al, 1998;Mitsiadis and Rahiotis, 2004). In culture, dental pulp cells are able to synthesize a specific matrix resembling dentin (Kasugai et al, 1993;Panagakos, 1998;Shiba et al, 1998;Onishi et al, 1999;About et al, 2000;Couble et al, 2000;Zhang et al, 2005). These cells are usually cultured in Eagle's basal medium, MEM, α-MEM or D-MEM and the supplementation with β-glycerophosphate or dexamethasone is a prerequisite to observe odontoblastlike differentiation, nodule formation and mineralization.…”

Section: Introductionmentioning

confidence: 99%

Culture medium modulates the behaviour of human dental pulp-derived cells: Technical Note

Lopez-Cazaux¹,

Bluteau²,

Magne³

et al. 2006

eCM

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In vitro approaches have extensively been developed to study reparative dentinogenesis. While dental pulp is a source of unidentified progenitors able to differentiate into odontoblast-like cells, we investigated the effect of two media; MEM (1.8mM Ca and 1mM Pi) and RPMI 1640 (0.8mM Ca and 5mM Pi) on the behaviour of human dental pulp cells. Our data indicate that MEM significantly increased cell proliferation and markedly enhanced the proportion of α-smooth muscle actin positive cells, which represent a putative source of progenitors able to give rise to odontoblast-like cells. In addition, MEM strongly stimulated alkaline phosphatase activity and was found to induce expression of transcripts encoding dentin sialophosphoprotein, an odontoblastic marker, without affecting that of parathyroid hormone/parathyroid hormone related protein-receptor and osteonectin. In conclusion, these observations demonstrate that not only proliferation but also differentiation into odontoblast-like cells was induced by rich calcium and poor phosphate medium (MEM) as compared to RPMI 1640. This study provides important data for the determination of the optimal culture conditions allowing odontoblast-like differentiation in human pulp cell culture.

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Human Dentin Production in Vitro

Cited by 248 publications

References 45 publications

Stem cells for tooth engineering

Stem cells for tooth engineering

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Culture medium modulates the behaviour of human dental pulp-derived cells: Technical Note

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