Human Dentin-matrix-derived Bone Morphogenetic Protein

: We first confirmed the osteoinductive property of human demineralized dentin matrix (DDM) histologically. Human DDM was prepared from vital, extracted teeth and implanted into the subcutaneous tissue in nude mice. The shape of the DDM was a particle type and its size varied from 0.4 to 0.8 mm. The hard tissue induction by 70 mg of the DDM was estimated at 4 weeks after implantation. The DDM induced bone and cartilage independently. In addition, the time-course of the bioassay by recombinant human BMP-2 (5.0µγ)/ DDM (70mg) was estimated in rat subcutaneous tissues. Histological examination showed that the BMP-2/ DDM induced bone and cartilage and the DDM were gradually replaced by new bone. The morphometric analysis demonstrated that the BMP-2/DDM showed 79.0% in the volume of bone and marrow, and 21.0% in that of DDM at 32 weeks. These results indicate that human DDM particles are osteoinductive matrics and the DDM might be effective as a carrier of BMP-2 for bone engineering.

Section: Resultsmentioning

confidence: 99%

Bone Engineering Using Human Demineralized Dentin Matrix and Recombinant Human BMP-2

Murata

2005

“…In 1991, a protein with BMP-like activity was isolated from humen dentin, but the NH 2 -terminal amino acid sequence did not resemble that of the known BMP family 29) . Moreover, amelogenin-polypeptides with chondrogenic / osteogenic-inducing activity were isolated from normally processed bovine dentine in 1999 30) , but unrelated to therefore, has been unclear until now.…”

Section: Bone Generation By Ddm Graftingmentioning

confidence: 99%

Bone Regeneration by Demineralized Dentin Matrix in Skull Defects of Rats

Togari

Miyazawa

Yagihashi

et al. 2012

Alveolar bone defects may result in functional and morphological abnormalities. In such cases, bone can be regenerated by grafting fresh autogenous bone into the defect. Subsequently, we focused on the dentin of third molars, which are usually discarded after extraction, or teeth removed faor orthodontic therapy. As in bone, type I collagen is the main component of the organic matrix in dentin. Also, it has been proven that the organic matrix of dentin contains osteoinductive bone morphogenetic protein (BMP). It would be safe to perform grafting with the dentin of extracted teeth used as an autogenous bone substitute. Therefore, the objective of the present study was to reuse extracted teeth as a bone substitute material. Demineralized dentin matrix (DDM), an organic material derived from the dentine of bovine teeth, was grafted in rat skull defects to determine its bone regeneration ability and the possibility of its use as a bone substitute. In the present study, new bone formed from the defect margin in the control group, but in the graft group, new bone formation occurred from individual DDM granules within the defect, and not just from the margin. At Week 8, defect repair was limited in the control group. In contrast, most of the defect was covered by osseous tissue in the graft group. In conclusion, DDM may be a useful bone substitute that serves as a scaffold for bone regeneration by inducing a high level of new bone formation soon after surgery.

“…To overcome these limitations, much effort has continued to develop more ideal bone grafting materials 1) . Based on many dentin reports, we have been interested in both the osteoinduction capacities of dentin and the possibilities of bio-recycling of teeth which considered as medical waste for a long dental history [1][2][3][4][5][6][7][8][9][10][11] . The use of autotooth bone graft materials, which are made from patient's own extracted teeth, is nowadays a standard dental procedure in Korea 2) .…”

Section: Introductionmentioning

confidence: 99%

Tooth Bank System for Bone Regeneration - Safety Report -

Kim¹,

Um²,

Murata³

2014

The use of auto-tooth bone grafts fabricated from patients' own extracted teeth has become possible due to the development of tooth banking procedures. The Korea Tooth Bank (KTB), established in Seoul in 2009, is one such tooth-banking facility that can procure and store teeth, and then process them into bone graft substitutes. Another is the Hospital Tooth Bank (HTB) at Seoul National University Bundang Hospital (SNUBH), established in 2010 for performing storage and grafting of auto-tooth bone grafts based on experimental and clinical research. Extracted teeth are sent to the above-mentioned facility units and then delivered back to the patients for clinical use, and thus the safety of the auto-tooth bone graft materials as well as the clinical effectiveness must be guaranteed through proper quality assurance (QA) procedures. For the purpose of this investigation, we analyzed written documents for QA at KTB, and we performed histopathologic and microbiologic examinations against the banked tooth materials at the HTB. The results suggest that the tooth banking systems at both KTB and HTB sufficiently ensure patient safety.