Human Cytomegalovirus UL24 and UL43 Cooperate to Modulate the Expression of Immunoregulatory UL16 Binding Protein 1

Sleman, Sirwan Salman; Hao, Huaiqing; Najmuldeen, Hastyar; Jalal, Paywast Jamal; Saeed, Nahla Muhammad; Othman, Dyary; Zhou, Qian

doi:10.1089/vim.2022.0041

Cited by 2 publications

(2 citation statements)

References 50 publications

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“…BAC technology has definitely advanced our knowledge of the life cycle of large DNA and RNA viruses. Sleman et al [ 95 ] constructed a double-knockout recombinant virus of human cytomegalovirus (HCMV) UL24 and UL43 based on the λ-red technique combined with the kana-I-sceI screening method. They demonstrated the potential role of UL24 and UL43 in regulating the host cell environment to facilitate immune escape by regulating the expression of downstream UL16 binding protein 1 ( ULBP1 ).…”

Section: Application Of Bac-based Gene Editing In Herpesvirus Researchmentioning

confidence: 99%

Bacterial-Artificial-Chromosome-Based Genome Editing Methods and the Applications in Herpesvirus Research

Hao

Tang

et al. 2023

Microorganisms

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Herpesviruses are major pathogens that infect humans and animals. Manipulating the large genome is critical for exploring the function of specific genes and studying the pathogenesis of herpesviruses and developing novel anti-viral vaccines and therapeutics. Bacterial artificial chromosome (BAC) technology significantly advanced the capacity of herpesviruses researchers to manipulate the virus genomes. In the past years, advancements in BAC-based genome manipulating and screening strategies of recombinant BACs have been achieved, which has promoted the study of the herpes virus. This review summarizes the advances in BAC-based gene editing technology and selection strategies. The merits and drawbacks of BAC-based herpesvirus genome editing methods and the application of BAC-based genome manipulation in viral research are also discussed. This review provides references relevant for researchers in selecting gene editing methods in herpes virus research. Despite the achievements in the genome manipulation of the herpes viruses, the efficiency of BAC-based genome manipulation is still not satisfactory. This review also highlights the need for developing more efficient genome-manipulating methods for herpes viruses.

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Section: Application Of Bac-based Gene Editing In Herpesvirus Researchmentioning

confidence: 99%

Bacterial-Artificial-Chromosome-Based Genome Editing Methods and the Applications in Herpesvirus Research

Hao

Tang

et al. 2023

Microorganisms

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“…Activates c-Jun [142] Immune evasion [139] UL43 Downregulates the expression of miR-UL59 [78] Immune evasion [78] UL44 DNA-binding nuclear protein, complexed to nucleolin and dsDNA [143]; Natural substrate of UL97 [144]; DNA polymerase accessory protein, increases DNA Pol processivity [145][146][147]; Inhibits p53 transcriptional activity [148]; Inhibits the binding of IRF3 and NF-κB to promoters of antiviral genes [149]; Sumoylation of UL44 attenuates viral replication [150,151] Immune evasion [149]…”

Section: Ul42mentioning

confidence: 99%

Insights into the Transcriptome of Human Cytomegalovirus: A Comprehensive Review

Zeng

Cao

Yang

et al. 2023

Viruses

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Human cytomegalovirus (HCMV) is a widespread pathogen that poses significant risks to immunocompromised individuals. Its genome spans over 230 kbp and potentially encodes over 200 open-reading frames. The HCMV transcriptome consists of various types of RNAs, including messenger RNAs (mRNAs), long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs), with emerging insights into their biological functions. HCMV mRNAs are involved in crucial viral processes, such as viral replication, transcription, and translation regulation, as well as immune modulation and other effects on host cells. Additionally, four lncRNAs (RNA1.2, RNA2.7, RNA4.9, and RNA5.0) have been identified in HCMV, which play important roles in lytic replication like bypassing acute antiviral responses, promoting cell movement and viral spread, and maintaining HCMV latency. CircRNAs have gained attention for their important and diverse biological functions, including association with different diseases, acting as microRNA sponges, regulating parental gene expression, and serving as translation templates. Remarkably, HCMV encodes miRNAs which play critical roles in silencing human genes and other functions. This review gives an overview of human cytomegalovirus and current research on the HCMV transcriptome during lytic and latent infection.

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Human Cytomegalovirus UL24 and UL43 Cooperate to Modulate the Expression of Immunoregulatory UL16 Binding Protein 1

Cited by 2 publications

References 50 publications

Bacterial-Artificial-Chromosome-Based Genome Editing Methods and the Applications in Herpesvirus Research

Bacterial-Artificial-Chromosome-Based Genome Editing Methods and the Applications in Herpesvirus Research

Insights into the Transcriptome of Human Cytomegalovirus: A Comprehensive Review

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