Human Corneal Donor Material

Manipulative damage to the endothelium of infant and adult donor corneas.

“…The incision was completed with curved corneal scissors and the ciliary body attachments to the scleral spur were separated. Care was taken to maintain the AC.7 Because the eyes of the very young donors (1,2,3 in Table I) were too small to fit into currently available commercial eye stands, they were wrapped in gauze and hand held during corneal excision. Some difficulty was encountered in these cases during separation of the ciliary body from the scleral spur.…”

Manipulative damage to the endothelium of infant and adult donor corneas.

“…The incision was completed with curved corneal scissors and the ciliary body attachments to the scleral spur were separated. Care was taken to maintain the AC.7 Because the eyes of the very young donors (1,2,3 in Table I) were too small to fit into currently available commercial eye stands, they were wrapped in gauze and hand held during corneal excision. Some difficulty was encountered in these cases during separation of the ciliary body from the scleral spur.…”

Corneal Endothelial Permeability Following Storage in Moist Chamber and Mk Medium

“…Previous work has demonstrated that human corneal endothelial permeability to sucrose and urea increased as a function of both time and age of the donor during storage at 4°C: in a moist chamber (Hoefle 1969). It has been demonstrated that the endothelial permeability of older donors increased at a more rapid rate than that of younger donors, and that the permeability of a 4-month-old donor was unchanged after 80 h of storage (Hoefle 1969). T h e unchanged permeability in healthy rabbit corneas used in this investigation may be compared to the unchanged permeability in the 4-month-old human cornea, It is currently not known if human corneas stored in MK medium undergo a change in endothelial permeability as do older eyes stored in moist chambers.…”

“…Previous work has demonstrated that human corneal endothelial permeability to sucrose and urea increased as a function of both time and age of the donor during storage at 4°C: in a moist chamber (Hoefle 1969). It has been demonstrated that the endothelial permeability of older donors increased at a more rapid rate than that of younger donors, and that the permeability of a 4-month-old donor was unchanged after 80 h of storage (Hoefle 1969).…”

Effects of Moist-Chamber and McCarey-Kaufman Medium Storage on the Metabolic Status of the Cornea: A 31 P-Magnetic Resonance Analysis

“…1964;Hoeffle, 1969;Sherrard, 1973;McCarcy andKaufman, 1974: McCarey, 1976;Friedland and Forster, 1976;Bin der, 1980] and excised cornea in McCareyKaufman (M-K) medium [McCarey and Kaufman, 1974;McCarey, 1976;McCarey et al, , 1976Stark et al, 1975;Van Horn et al, 1975;Breslin et al, 1976;Friedland and Forster, 1976;Aquavella et al, 1976;Binder and Wickham, 1978;Binder, 1980;Sayegh, 1982;Harbour and Stern, 1983;Neubauer et al, 1984] at 4°C have suggested that the maximum storage time for each technique is 24-48 h and 96 h respec tively. Although clinical experience within these limits has been successful with each of these storage methods, there remains little and conflicting information on the meta bolic status of the cornea and on the dura tion of corneal viability.…”

Manipulative damage to the endothelium of infant and adult donor corneas.

“…The incision was completed with curved corneal scissors and the ciliary body attachments to the scleral spur were separated. Care was taken to maintain the AC.7 Because the eyes of the very young donors (1,2,3 in Table I) were too small to fit into currently available commercial eye stands, they were wrapped in gauze and hand held during corneal excision. Some difficulty was encountered in these cases during separation of the ciliary body from the scleral spur.…”

Corneal Endothelial Permeability Following Storage in Moist Chamber and Mk Medium

“…Previous work has demonstrated that human corneal endothelial permeability to sucrose and urea increased as a function of both time and age of the donor during storage at 4°C: in a moist chamber (Hoefle 1969). It has been demonstrated that the endothelial permeability of older donors increased at a more rapid rate than that of younger donors, and that the permeability of a 4-month-old donor was unchanged after 80 h of storage (Hoefle 1969). T h e unchanged permeability in healthy rabbit corneas used in this investigation may be compared to the unchanged permeability in the 4-month-old human cornea, It is currently not known if human corneas stored in MK medium undergo a change in endothelial permeability as do older eyes stored in moist chambers.…”

“…Previous work has demonstrated that human corneal endothelial permeability to sucrose and urea increased as a function of both time and age of the donor during storage at 4°C: in a moist chamber (Hoefle 1969). It has been demonstrated that the endothelial permeability of older donors increased at a more rapid rate than that of younger donors, and that the permeability of a 4-month-old donor was unchanged after 80 h of storage (Hoefle 1969).…”

Effects of Moist-Chamber and McCarey-Kaufman Medium Storage on the Metabolic Status of the Cornea: A 31 P-Magnetic Resonance Analysis

“…1964;Hoeffle, 1969;Sherrard, 1973;McCarcy andKaufman, 1974: McCarey, 1976;Friedland and Forster, 1976;Bin der, 1980] and excised cornea in McCareyKaufman (M-K) medium [McCarey and Kaufman, 1974;McCarey, 1976;McCarey et al, , 1976Stark et al, 1975;Van Horn et al, 1975;Breslin et al, 1976;Friedland and Forster, 1976;Aquavella et al, 1976;Binder and Wickham, 1978;Binder, 1980;Sayegh, 1982;Harbour and Stern, 1983;Neubauer et al, 1984] at 4°C have suggested that the maximum storage time for each technique is 24-48 h and 96 h respec tively. Although clinical experience within these limits has been successful with each of these storage methods, there remains little and conflicting information on the meta bolic status of the cornea and on the dura tion of corneal viability.…”

Manipulative damage to the endothelium of infant and adult donor corneas.

“…The incision was completed with curved corneal scissors and the ciliary body attachments to the scleral spur were separated. Care was taken to maintain the AC.7 Because the eyes of the very young donors (1,2,3 in Table I) were too small to fit into currently available commercial eye stands, they were wrapped in gauze and hand held during corneal excision. Some difficulty was encountered in these cases during separation of the ciliary body from the scleral spur.…”

Corneal Endothelial Permeability Following Storage in Moist Chamber and Mk Medium

“…Previous work has demonstrated that human corneal endothelial permeability to sucrose and urea increased as a function of both time and age of the donor during storage at 4°C: in a moist chamber (Hoefle 1969). It has been demonstrated that the endothelial permeability of older donors increased at a more rapid rate than that of younger donors, and that the permeability of a 4-month-old donor was unchanged after 80 h of storage (Hoefle 1969). T h e unchanged permeability in healthy rabbit corneas used in this investigation may be compared to the unchanged permeability in the 4-month-old human cornea, It is currently not known if human corneas stored in MK medium undergo a change in endothelial permeability as do older eyes stored in moist chambers.…”

“…Previous work has demonstrated that human corneal endothelial permeability to sucrose and urea increased as a function of both time and age of the donor during storage at 4°C: in a moist chamber (Hoefle 1969). It has been demonstrated that the endothelial permeability of older donors increased at a more rapid rate than that of younger donors, and that the permeability of a 4-month-old donor was unchanged after 80 h of storage (Hoefle 1969).…”

Effects of Moist-Chamber and McCarey-Kaufman Medium Storage on the Metabolic Status of the Cornea: A 31 P-Magnetic Resonance Analysis

“…1964;Hoeffle, 1969;Sherrard, 1973;McCarcy andKaufman, 1974: McCarey, 1976;Friedland and Forster, 1976;Bin der, 1980] and excised cornea in McCareyKaufman (M-K) medium [McCarey and Kaufman, 1974;McCarey, 1976;McCarey et al, , 1976Stark et al, 1975;Van Horn et al, 1975;Breslin et al, 1976;Friedland and Forster, 1976;Aquavella et al, 1976;Binder and Wickham, 1978;Binder, 1980;Sayegh, 1982;Harbour and Stern, 1983;Neubauer et al, 1984] at 4°C have suggested that the maximum storage time for each technique is 24-48 h and 96 h respec tively. Although clinical experience within these limits has been successful with each of these storage methods, there remains little and conflicting information on the meta bolic status of the cornea and on the dura tion of corneal viability.…”