Human bronchial intraepithelial T cells produce interferon-γ and stimulate epithelial cells

Hirosako, Susumu; Goto, Eisuke; Fujii, Kenichi; Tsumori, Kaori; Hirata, Naomi; Tsumura, S.; Kamohara, Hidenobu; Kohrogi, Hirotsugu

doi:10.1111/j.1365-2249.2008.03811.x

Cited by 5 publications

(12 citation statements)

References 33 publications

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“…However, our findings in this group are comparable to data published by Hirosako et al, who have meticulously analyzed CD103 + expression by intraepithelial T cells in human bronchi (third to fifth generation) in a patient group that is demographically very similar to our cohort (14,16). They showed that a range of 74% to 94% of intraepithelial CD8 + cells expressed CD103, whereas only 8% to 25% of lamina propria T cells expressed CD103.…”

Section: Discussionsupporting

confidence: 91%

“…Immunohistochemistry confirmed that most lung CD103 + CD3 + CD8 + T cells were indeed located intraepithelially above the basement membrane of the small airways ( Figure 1C), as was previously published (13)(14)(15)(16).…”

Section: Human Lung Cd8 + T Cells Expressing αE Integrin Are Ielssupporting

confidence: 86%

“…Intraepithelial CD8 + T cells express αEβ7 integrin, which binds to epithelial cadherin (E-cadherin), expressed by the epithelial cells (11)(12)(13)(14)(15)(16). This interaction retains the IELs in the vicinity of the epithelium (17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

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CD8+ T cells with an intraepithelial phenotype upregulate cytotoxic function upon influenza infection in human lung

Piet¹,

Bree²,

et al. 2011

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The human lung T cell compartment contains many CD8 + T cells specific for respiratory viruses, suggesting that the lung is protected from recurring respiratory infections by a resident T cell pool. The entry site for respiratory viruses is the epithelium, in which a subset of lung CD8 + T cells expressing CD103 (αE integrin) resides. Here, we determined the specificity and function of CD103 + CD8 + T cells in protecting human lung against viral infection. Mononuclear cells were isolated from human blood and lung resection samples. Variable numbers of CD103 + CD8 + T cells were retrieved from the lung tissue. Interestingly, expression of CD103 was seen only in lung CD8 + T cells specific for influenza but not in those specific for EBV or CMV. CD103 + and influenza-reactive cells preferentially expressed NKG2A, an inhibitor of CD8 + T cell cytotoxic function. In contrast to CD103 -CD8 + T cells, most CD103 + CD8 + cells did not contain perforin or granzyme B. However, they could quickly upregulate these cytotoxic mediators when exposed to a type I IFN milieu or via contact with their specific antigen. This mechanism may provide a rapid and efficient response to influenza infection, without inducing cytotoxic damage to the delicate epithelial barrier.

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Section: Discussionsupporting

confidence: 91%

Section: Human Lung Cd8 + T Cells Expressing αE Integrin Are Ielssupporting

confidence: 86%

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CD8+ T cells with an intraepithelial phenotype upregulate cytotoxic function upon influenza infection in human lung

Piet¹,

Bree²,

et al. 2011

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“…Subsequently, we isolated bronchial IELs and LPLs from resected lung tissue and showed that IELs produced γ-interferon, which stimulated bronchial epithelial cells, and that IELs could be maintained by epithelial cells [12]. However, to date, a comparative evaluation of phenotypes of both bronchial IELs and LPLs in both normal and pathological states has not been made.…”

Section: Introductionmentioning

confidence: 99%

“…However, such processes can alter the expression of some cellular surface markers [14]. Furthermore, isolation of bronchial IELs using conventional separation methods is challenging and requires short-time incubation of bronchial specimens with interleukin (IL) 2 [12] due to smaller sample size and complex branching structure of the tissue. Such treatment may further alter the cells’ surface antigen profile.…”

Section: Introductionmentioning

confidence: 99%

CD8 and CD103 Are Highly Expressed in Asthmatic Bronchial Intraepithelial Lymphocytes

Hirosako

Goto²,

Tsumori³

et al. 2010

Int Arch Allergy Immunol

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Background: Although characteristics of intraepithelial lymphocytes (IELs) in mucosal immunity have been well defined in the intestine, bronchial IELs have been little investigated. Recently, we showed that bronchial IELs have a distinct function that partly resembles that of intestinal IELs; however, surface antigen expression of bronchial IELs and the relationship of that expression to airway disease have not been studied. Methods: We analyzed phenotypic profiles of human bronchial IELs and lamina propria lymphocytes (LPLs) by double-staining immunohistochemistry using full-thickness bronchial specimens (10 nonasthmatic controls and 7 asthmatics) from lung resections. Results: In controls, the percentage of CD4+ cells was lower, and the percentage of CD8+ cells was higher in IELs compared to LPLs (CD4: median 50.0% in IELs vs. 65.9% in LPLs, p = 0.01; CD8: 50.9% in IELs vs. 34.4% in LPLs, p = 0.007). The percentage of cells positive for CD103 (αE-integrin) was higher in IELs than that in LPLs (median 60.1% in IELs vs. 16.9% in LPLs; p < 0.001). In IELs from asthmatics, these characteristics were particularly significant (CD4: median 26.2%, p = 0.008; CD8: 79.8%, p = 0.007; CD103: 76.2%, p = 0.019; all compared with IELs from nonasthmatics). Conclusions: These results suggest that human bronchial IELs have roles distinct from subsets of other lymphocytes, and that CD8+ cells and CD103+ cells have potentially important functions in the bronchial epithelium.

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Morphology of Non-Sensory Epithelium during Post-natal Development of the Rabbit Vomeronasal Organ

Elgayar

Eltony

Othman

2013

Anat. Histol. Embryol.

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The vomeronasal organ (VNO), because of its ability to detect pheromones, has an important role in many social and sexual behaviours in mammals. It also mediates defensive behaviours through detection of protein pheromone homologues. A detailed morphological description of the post-natal development of the 'non-sensory' epithelium (NSE) of the female rabbit is recorded. Histological techniques were used to study the NSE of the VNO in post-natal development of female rabbits. The study focused on the following post-natal ages: newborn, 1 week, 2 weeks and 1 month (five animals each) beside to two adult animals. The rabbit VNO was surrounded externally by bony capsule and internally by cartilaginous capsule. NSE was pseudostratified columnar partially ciliated epithelium without goblet cells. In addition to basal cells, NSE contained ciliated and three types of non-ciliated columnar cells (dark, pale and light). At birth, dark cells may have primary cilia. By 1 month, the cytoplasm became lighter with less free ribosomes. The pale cells had electron-lucent cytoplasm, which contained a few organelles. Mitotic figures were observed in basal and columnar cells, particularly during the first 2 weeks of post-natal development. Light columnar cells were common during the first week. Numerous leucocytes and a few nerve endings were detected intra-epithelial. Scanning electron microscope revealed a gradual increase in height of microvilli of non-ciliated cells. Ciliated cells had cilia and microvilli. Cells were arranged singly, in clumps or in a dense population of cells. The rabbit VNO-NSE had a unique morphological structure.

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Human bronchial intraepithelial T cells produce interferon-γ and stimulate epithelial cells

Cited by 5 publications

References 33 publications

CD8+ T cells with an intraepithelial phenotype upregulate cytotoxic function upon influenza infection in human lung

CD8+ T cells with an intraepithelial phenotype upregulate cytotoxic function upon influenza infection in human lung

CD8 and CD103 Are Highly Expressed in Asthmatic Bronchial Intraepithelial Lymphocytes

Morphology of Non-Sensory Epithelium during Post-natal Development of the Rabbit Vomeronasal Organ

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