Human Adenovirus Serotypes Efficiently Transducing HEK293 Cells: An In Vitro Propagation of HAdv

Abstract

Generally, a gene which is inserted directly into a cell does not operate independently. Instead, the transmission of the gene is genetically modified by a biological messenger called a vector, consists of a transgene and a large DNA sequence as a backbone. Since they can deliver the new gene by infecting the cell, such viruses are also used as vectors. The adenovirus is a non-enveloped virus that can be tailored to transfer DNA to target cells, and it has sparked a lot of interest in the field, particularly in clinical trial therapy techniques. For the new age production of COVID-19 vaccine, development of different mammalian cell lines like HEK293 (most reliable growth and prosperity for transfection) and recombinant adenoviral vectors have become the first priority for biopharmaceutical giants and globally approved vaccine manufacturers to scale up their vaccine production. Adenoviruses have an icosahedral shape, with a protein coat encasing the viral double-stranded DNA genome. Because the adenovirus genome is relatively small, it's a good candidate for insertion of foreign DNA. The adenovirus E1A gene is deleted, and the virus loses its capacity to replicate. This ability can be restored during cell culture propagation by employing cells that produce the E1A protein, for example. Hence, in this mini research, I have shared an overview of the propagation of adenoviral vectors, i.e. recombinant adenovirus SARS CoV-2 vector in HEK-293 cell suspension culture.

Phenolic-rich feijoa extracts from flesh, peel and whole fruit activate apoptosis pathways in the LNCaP cell line

“…After four doubling cycles, the 50% inhibitory concentrations (IC50) of the anti-proliferative activity of feijoa extracts were determined using the Sulforhodamine B (SRB) assay as reported previously (Peng et al, 2018). The HEK293 cell line has been widely used in anticancer studies due to its reliable growth and high transfection efficiency (Samantaray & Santra, 2021). In particular, it is employed as a noncancerous control for many cancer-specific cells to determine the cytotoxicity of functional food ingredients (Xu, Gao, & Granato, 2021;Cerda-Opazo, Gotteland, Oyarzun-Ampuero, & Garcia, 2021).…”

Human Mastadenovirus Infections in Children: A Review of the Current Status in the Arab World in the Middle East and North Africa

“…Human mastadenovirus (HAdV) is a non-enveloped icosahedral virus with double-stranded DNA genomes [ 1 ]. Since Wallace P. Rowe isolated and named the adenoviral serotype HAdV from human tonsils and adenoids in 1953, more than 120 distinct adenoviral serotypes have been discovered in humans, animals, birds, fish, and reptiles.…”