volume 18, issue 2, P184-191 2018
DOI: 10.1590/1984-70332018v18n2a26
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Abstract: The clustered regularly interspaced short palindromic repeat (CRISPR)/ CRISPR-associated (Cas) technology has recently emerged as a powerful genomic editing tool with great potential for crop breeding. However, commonly used protocols for screening of CRISPR/Cas9-induced mutations are laborious, timeconsuming, and costly. In the present study we examined the applicability of high resolution melting (HRM) analysis fast screening CRISPR/Cas9-induced mutations in T 0 plants and subsequent genotyping of T 1 popula…

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