HPV16 Variants in Squamous Intraepithelial Lesions in Human Immunodeficiency Virus–Negative and –Positive Brazilian Women

Gheit, Tarik; Simões, Renata Toscano; Tommasino, Massimo; Donadi, Eduardo Antônio; Guimaratilde, Maria Alice; Gonçalves, es

doi:10.1089/vim.2006.19.340

Cited by 6 publications

(4 citation statements)

References 38 publications

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“…Seven out of eleven HPV16 sequences from cervical samples analyzed in the present study were classified as variant A. This variant distribution is in agreement with previous Brazilian studies, which observed that 34% (Gheit et al, 2006) to 65.8% (Freitas et al, 2014) of the HPV16 isolates are classified as HPV16_A variant. We could ascribe the SNPs in the different isolates here sequenced to the differential variant signatures (Smith et al, 2011).…”

Section: Discussionsupporting

confidence: 92%

High-level of viral genomic diversity in cervical cancers: A Brazilian study on human papillomavirus type 16

Oliveira

Bravo

Souza

et al. 2015

Infection, Genetics and Evolution

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Invasive cervical cancer (ICC) is the third most frequent cancer among women worldwide and is associated with persistent infection by carcinogenic human papillomaviruses (HPVs). The combination of large populations of viral progeny and decades of sustained infection may allow for the generation of intra-patient diversity, in spite of the assumedly low mutation rates of PVs. While the natural history of chronic HPVs infections has been comprehensively described, within-host viral diversity remains largely unexplored. In this study we have applied next generation sequencing to the analysis of intra-host genetic diversity in ten ICC and one condyloma cases associated to single HPV16 infection. We retrieved from all cases near full-length genomic sequences. All samples analyzed contained polymorphic sites, ranging from 3 to 125 polymorphic positions per genome, and the median probability of a viral genome picked at random to be identical to the consensus sequence in the lesion was only 40%. We have also identified two independent putative duplication events in two samples, spanning the L2 and the L1 gene, respectively. Finally, we have identified with good support a chimera of human and viral DNA. We propose that viral diversity generated during HPVs chronic infection may be fueled by innate and adaptive immune pressures. Further research will be needed to understand the dynamics of viral DNA variability, differentially in benign and malignant lesions, as well as in tissues with differential intensity of immune surveillance. Finally, the impact of intralesion viral diversity on the long-term oncogenic potential may deserve closer attention.

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Section: Discussionsupporting

confidence: 92%

High-level of viral genomic diversity in cervical cancers: A Brazilian study on human papillomavirus type 16

Oliveira

Bravo

Souza

et al. 2015

Infection, Genetics and Evolution

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“…Another study, with women from Northern Brazil found NE variants associated with high-grade cervical lesions [42]. However, HPV16 NE variants were detected at similar frequencies in low grade lesions (6/41, 14.6%) and in high grade cases (4/41, 9.7%) in a study conducted in São Paulo, also in Southeastern Brazil [39] and HPV16 NE and E variants have been detected at similar frequencies among the cytological finds (atypical squamous or glandular cells of undetermined significance, cytological alterations suggesting HPV infection, CIN, squamous cell carcinoma, and adenocarcinoma) in women from Central Brazil [38], not supporting a role for NE HPV16 variants as at increased risk for CC. Nevertheless, there is other evidence that HPV16 NE variants have elevated risks for CIN3 and cancer, although much of the effect was related to the increased risk with the AA (D) lineage [25], [56], [66], and there appears to be geographic complexity [58].…”

Section: Discussionmentioning

confidence: 91%

Human Papillomavirus 16 Non-European Variants Are Preferentially Associated with High-Grade Cervical Lesions

et al. 2014

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HPV16 accounts for 50–70% of cervical cancer cases worldwide. Characterization of HPV16 variants previously indicated that they differ in risks for viral persistence, progression to cervical precancer and malignant cancer. The aim of this study was to examine the association of severity of disease with HPV16 variants identified in specimens (n = 281) obtained from a Cervical Pathology and Colposcopy outpatient clinic in the University Hospital of Espírito Santo State, Southeastern Brazil, from April 2010 to November 2011. All cytologic and histologic diagnoses were determined prior to definitive treatment. The DNA was isolated using QIAamp DNA Mini Kit and HPV was detected by amplification with PGMY09/11 primers and positive samples were genotyped by RFLP analyses and reverse line blot. The genomes of the HPV16 positive samples were sequenced, from which variant lineages were determined. Chi2 statistics was performed to test the association of HPV16 variants between case and control groups. The prevalence of HR-HPV types in

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“…HIV infection increases the risk of cervical infection due to high-risk HPV genotypes that induces high-grade cervical squamous intraepithelial lesions (HSILs), which in turn leads to the development of pre-invasive cervical lesions and invasive cervical cancer (ICC) [23][24][25]. HIV infection could alter the natural history of HPV infection through decreasing the self-clearance rate of infection and increasing progression to high grade and invasive lesions [24].…”

Section: Introductionmentioning

confidence: 99%

“…HIV infection could alter the natural history of HPV infection through decreasing the self-clearance rate of infection and increasing progression to high grade and invasive lesions [24]. Furthermore, the incidence of HPV infection is three times higher in HIV-positive women [25], and can cause cervical cancer than their counterparts [26]. Nonetheless, with the exception of the systematic review and meta-analysis done in Kenya [27], evidences in this regard showing the burden and molecular distribution of HPV in low and middle income countries (LMICs) is limited [28].…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of human papillomavirus among HIV infected women in developing countries: systematic review and meta-analysis

Bogale

Belay

Medhin

et al. 2020

Virol J

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Background Although, there is a variable burden of human papillomavirus (HPV) in women infected with HIV in developing countries, there are few studies that attempted to surmise such variable evidences. This review aimed to estimate the pooled prevalence of HPV genotype distribution and risk factors contributing to HPV infection among women infected with HIV in low- and middle-income countries. Methods We conducted a systematic review and meta-analysis of studies conducted in developing countries and reported HPV prevalence. We searched electronic databases: PubMed/Medline, SCOPUS, ScienceDirect, Excerpta Medical Database from Elsevier, Web of science, Cumulative Index of Nursing and allied Health Sciences and Google scholar databases to retrieve primary studies published in English language till 11th August 2019. We used random-effects model to estimate the pooled prevalence of HPV genotypes, and funnel plot to assess publication bias. The registration number of this review study protocol is CRD42019123549. Results We included nineteen studies with a total of 8,175 participants in this review. The prevalence of HPV was extremely heterogeneous across the studies (χ2= 3782.80, p value < 0.001, I2 = 99.6%). The estimated pooled prevalence of all HPV genotypes was 63.0% (95% CI: 48.0–78.0) while the pooled prevalence of high risk and low risk HPV genotypes were 51.0% (95% CI: 38.0–63.0) and 28.0% (95% CI: 12.0–43.0), respectively. The pooled prevalence of HPV genotype 16 was 20%, while genotype 18 and 52 were 15% and 13%, respectively. Different risk factors reported for HPV infection and the frequently reported were low CD4 count below 200 cells/mm3 and high HIV viral load. Conclusion The pooled prevalence of HPV among HIV infected women in low- and middle-income countries was considerable and the proportion of high risk HPV genotypes were high when compared with low risk genotypes. Therefore, it is essential for the HPV prevention program to prevent the double burden of HPV and HIV in women.

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HPV16 Variants in Squamous Intraepithelial Lesions in Human Immunodeficiency Virus–Negative and –Positive Brazilian Women

Cited by 6 publications

References 38 publications

High-level of viral genomic diversity in cervical cancers: A Brazilian study on human papillomavirus type 16

High-level of viral genomic diversity in cervical cancers: A Brazilian study on human papillomavirus type 16

Human Papillomavirus 16 Non-European Variants Are Preferentially Associated with High-Grade Cervical Lesions

Molecular epidemiology of human papillomavirus among HIV infected women in developing countries: systematic review and meta-analysis

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