HPTLC determination of ergosterol in wheat and structure elucidation by NMR: Toward confirming method selectivity

Ibrahim, Alyaa Hatem; Oraby, Mohamed; Khorshed, Ahmed Abdelhamid

doi:10.1016/j.jfca.2022.104763

Cited by 7 publications

(3 citation statements)

References 46 publications

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“…Since ergosterol is found almost exclusively in the cell membranes of fungi, it may serve as a biomarker for fungal growth. Therefore, ergosterol can also be used to exclude unintended fungal infestation of the substrate prior to use as a fermentation substrate (Ibrahim et al., 2022 ; Osswald et al., 1986 ). The crude protein content increased until main culture day 8 (up to 22.2 ± 0.1 g/100 g DM; no significant difference to main culture day 10, p < .05) as well as the fungal content (up to 54%; 233 mg/100 g DM ergosterol; no significant difference to main culture day 10, p < .05) (Figure 1 ).…”

Section: Resultsmentioning

confidence: 99%

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Fermentation of cocoa pod husks with Pleurotus salmoneo‐stramineus for food applications

Bickel Haase,

Klis,

Hammer

et al. 2024

Food Science & Nutrition

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Cocoa pod husks (CPHs), the major side‐stream from cocoa production, were valorized through fermentation with Pleurotus salmoneo‐stramineus (PSS). Considering ergosterol as a biomarker for the fungal content, the mycelium accounted for 54% of the total biomass after 8 days in submerged cultures. The crude protein content of fermented CPH (CPHF) increased from 7.3 g/100 g DM in CPH to 18.9 g/100 g DM. CPH fermentation resulted in a high biological value of 86 for the protein. The water and oil binding capacities of CPHF were 3.5 mL/g and 2.1 mL/g, respectively. The particle diameter dv,0,90 of CPHF was 373 μm as compared to 526 μm for CPH. The total dietary fiber was 73.4 g/100 g DM in CPHF and 63.6 g/100 g DM in CPH. The amount of soluble fiber was 2.3 g/100 g DM in CPHF and 10.1 g/100 g DM in CPH; the insoluble fraction accounted for 71.1 g/100 g DM and 53.6 g/100 g DM, respectively. Bread doughs with CPH or CPHF were characterized for texture, color, and farinographic properties. The dough hardness, consistency, and browning index increased with the concentration of CPH, whereas for CPHF, springiness and peak viscosities declined. We demonstrate the upcycling of CPH into nutritious and functional ingredients through PSS fermentation.

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Section: Resultsmentioning

confidence: 99%

“…Therefore, ergosterol can also be used to exclude unintended fungal infestation of the substrate prior to use as a fermentation substrate (Ibrahim et al, 2022;Osswald et al, 1986). The crude protein content increased until main culture day 8 (up to 22.2 ± 0.1 g/100 g DM;…”

Section: Submerged Cultivation Of Pss In Cph-mediummentioning

confidence: 99%

Fermentation of cocoa pod husks with Pleurotus salmoneo‐stramineus for food applications

Bickel Haase,

Klis,

Hammer

et al. 2024

Food Science & Nutrition

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“…The main compound (3.0 mg) was purified by the same HPLC condition. It was identified as ergosterol by an analysis of 1 H and 13 C NMR contrast the reference ( Appendix B Figure A2 and Appendix C Figure A3 ) [ 42 ]. Ergosterol, the major product of mycosterol biosynthesis, is an important component of fungal cell membranes that maintain membrane structural integrity, permeability, and fluidity, which can promote the absorption of calcium and phosphorus in the human body.…”

Section: Resultsmentioning

confidence: 99%

A New Species Agrocybe striatipes, also a Newly Commercially Cultivated Mushroom with Highly Nutritional and Healthy Values

Yang

Ren

et al. 2023

JoF

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The species of Agrocybe (Strophariaceae, Agaricales, Agaricomycetes) are saprophytic and widely distributed in temperate regions. In this study, a new species named Agrocybe striatipes from China is described, which has been successfully cultivated in China recently. The phenotypic characteristics examination and molecular phylogenetic analyses using multilocus data (ITS and nrLSU) both support it as a new species in the genus Agrocybe. Moreover, nutritional ingredient analysis showed that the fruiting body of A. striatipes was rich in seventeen amino acids, including eight essential amino acids, in addition to high levels of calcium (78.5 mg/kg) and vitamin D (44.1 μg/100g). The following analysis of the heavy metal contents of the fruiting bodies show that it does not contain lead, cadmium, arsenic, mercury, and other heavy metal elements. In the crude extract of the mushroom, the nutrients in the aqueous phase are amino acids and oligosaccharides, and the active substances in the ethyl acetate layer are sterols, which have a variety of pharmacological effects. In conclusion, A. striatipes is not only a new species but also has highly application values as a cultivated edible mushroom in nutrition and health.

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Development of a high-performance thin-layer chromatography-based method for targeted glycerolipidome profiling of microalgae

Makay,

Griehl,

Grewe

2024

Anal Bioanal Chem

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The conditionally essential very-long-chain polyunsaturated fatty acids (VLC-PUFAs), such as eicosapentaenoic acid (EPA, C20:5 n-3), play a vital role in human nutrition. Their biological activity is thereby greatly influenced by the distinct glycerolipid molecule that they are esterified to. Here, microalgae differ from the conventional source, fish oil, both in quantity and distribution of VLC-PUFAs among the glycerolipidome. Therefore, the aim of this study was to develop a fast and reliable one-dimensional high-performance thin-layer chromatography (HPTLC)-based method that allows the separation and quantification of the main microalgal glycerolipid classes (e.g., monogalactosyldiacylglycerol (MGDG), sulfoquinovosyl diacylglycerol (SQDG), phosphatidylglycerol (PG)), as well as the subsequent analysis of their respective fatty acid distribution via gas chromatography (GC) coupled to mass spectrometry (MS). Following optimization, method validation was carried out for 13 different lipid classes, based on the International Conference on Harmonization (ICH) guidelines. In HPTLC, linearity was effective between 100 and 2100 ng, with a limit of quantification between 62.99 and 90.09 ng depending on the glycerolipid class, with strong correlation coefficients (R2 > 0.995). The recovery varied between 93.17 and 108.12%, while the inter-day precision measurements showed coefficients of variation of less than 8.85%, close to the limit of detection. Applying this method to crude lipid extracts of four EPA producing microalgae of commercial interest, the content of different glycerolipid classes was assessed together with the respective FA distribution subsequent to band elution. The results showed that the described precise and accurate HPTLC method offers the possibility to be used routinely to follow variations in the glycerolipid class levels throughout strain screening, cultivation, or bioprocessing. Thus, additional quantitative analytical information on the complex lipidome of microalgae can be obtained, especially for n-3 and n-6 enriched lipid fractions. Graphical Abstract

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HPTLC determination of ergosterol in wheat and structure elucidation by NMR: Toward confirming method selectivity

Cited by 7 publications

References 46 publications

Fermentation of cocoa pod husks with Pleurotus salmoneo‐stramineus for food applications

Fermentation of cocoa pod husks with Pleurotus salmoneo‐stramineus for food applications

A New Species Agrocybe striatipes, also a Newly Commercially Cultivated Mushroom with Highly Nutritional and Healthy Values

Development of a high-performance thin-layer chromatography-based method for targeted glycerolipidome profiling of microalgae

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