HPTLC‐densitometric method of determination of oleanolic acid in the <i>Lamii albi flos</i>

Wójciak–Kosior, Magdalena; Krzaczek, T.; Matysik, G.; Skalska-Kamińska, Agnieszka

doi:10.1002/jssc.200500106

Cited by 9 publications

(4 citation statements)

References 16 publications

(11 reference statements)

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“…Some of the triterpenes identified in the L. album flowers in the present study have been described earlier in this species by other authors, e.g., oleanolic acid and β-amyrin [ 35 , 36 , 37 ]. In turn, the presence of β-amyrin acetate in the flowers of this species was demonstrated in this study for the first time.…”

Section: Discussionsupporting

confidence: 77%

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Histochemical and Phytochemical Analysis of Lamium album subsp. album L. Corolla: Essential Oil, Triterpenes, and Iridoids

et al. 2021

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The aim of this study was to conduct a histochemical analysis to localize lipids, terpenes, essential oil, and iridoids in the trichomes of the L. album subsp. album corolla. Morphometric examinations of individual trichome types were performed. Light and scanning electron microscopy techniques were used to show the micromorphology and localization of lipophilic compounds and iridoids in secretory trichomes with the use of histochemical tests. Additionally, the content of essential oil and its components were determined using gas chromatography-mass spectrometry (GC-MS). Qualitative analyses of triterpenes carried out using high-performance thin-layer chromatography (HPTLC) coupled with densitometric detection, and the iridoid content expressed as aucubin was examined with spectrophotometric techniques. We showed the presence of iridoids and different lipophilic compounds in papillae and glandular and non-glandular trichomes. On average, the flowers of L. album subsp. album yielded 0.04 mL/kg of essential oil, which was dominated by aldehydes, sesquiterpenes, and alkanes. The extract of the L. album subsp. album corolla contained 1.5 × 10−3 ± 4.3 × 10−4 mg/mL of iridoid aucubin and three triterpenes: oleanolic acid, β-amyrin, and β-amyrin acetate. Aucubin and β-amyrin acetate were detected for the first time. We suggest the use of L. album subsp. album flowers as supplements in human nutrition.

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Section: Discussionsupporting

confidence: 77%

“…They have anticancer, anti-inflammatory, antimicrobial, antitumoral, antiulcerogenic, and antiviral activity [ 30 , 31 , 32 , 33 , 34 ]. Triterpenes detected in L. album flowers are represented by oleanolic acid, ursolic acid, and β-amyrin [ 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

Histochemical and Phytochemical Analysis of Lamium album subsp. album L. Corolla: Essential Oil, Triterpenes, and Iridoids

et al. 2021

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“…[12] For the analysis of two compounds, thin layer chromatography (TLC) and high performance TLC (HP-TLC) have previously been used but required iodine or sulfuric acid as visualizing regent;[34] cyclodextrin-modified capillary micellar electrokinetic chromatography was a hybrid technique to high-speed separate them but difficult to scale up;[5] gas chromatography (GC) has once been used but required silylation or methylation derivatisation. [67] Up to date, only reverse phase liquid chromatography (RPLC) has widely been used to determinate them and proved to be the effective and convenient separation method.…”

Section: Introductionmentioning

confidence: 99%

Determination of oleanolic acid and ursolic acid in Chinese medicinal plants using HPLC with PAH polymeric C18

Yong

Ke-min²,

Zhao³

et al. 2013

Phcog Mag

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Background:The RP-HPLC resolution of two triterpenic acid isomers was unstable. Objective: To separate the oleanolic acid (OA) and ursolic acid (UA) simply within RP-HPLC.Materials and Methods:The separation ability of five stationary phases was studied with the retention effect of their carbon loads. Also the resolution effects of mobile phase composition and different column temperatures were systematically investigated by using Drylab® (Rheodyne LLC.) after evaluating chromatograms automatically.Results:The best available resolution of two bioactive isomers was achieved (r = 3.4) via using PAH (polycyclic aromatic hydrocarbons) polymeric C18 bonded phase column. The chromatographic system was applied to the quantification in ten Chinese medicinal plants and the validation was carried out and the precision (RSD ≤1.34%), the linearity (r ≥ 0.9998) and the recovery (range from 92.1% to 102.6%) were acceptable.Conclusion:It is clear that the method was simple, rapid and reliable for the quantification of two compounds in new HPLC method within PAH polymeric C18.

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“…In a measurement of absorbance, in 1 g of dry Lamii albi flos L., 1458 µg of oleanolic acid was gauged (0.147%) and using the fluorescence method, in 1 g of the plant material, 1516 µg of oleanolic acid was determined (0.149%). [ 24 ] Heptane extract of Lamium album especially had a high concentration of triterpenes. [ 14 ] The biological effect of natural compounds strongly depends not only on their concentration but also on the cell type they influence, their mode of cellular membrane interactions or time of intracellular uptake.…”

Section: Discussionmentioning

confidence: 99%

The effect of Lamium album extract on cultivated human corneal epithelial cells (10.014 pRSV-T)

Paduch¹,

Woźniak²

2015

J Ophthalmic Vis Res

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Purpose:To evaluate the effect of Lamium album extract on human corneal epithelial cells (10.014 pRSV-T cell line) cultured in vitro.Methods:Normal human corneal epithelial cells were incubated with ethanol, ethyl acetate and heptane extracts from Lamium album. Their effect on cells was evaluated by neutral red (NR) uptake and MTT assays for cytotoxicity, ELISA for immunomodulation, Griess method for nitric oxide levels, DPPH assay for free radicals scavenging activity. A blank control consisted only of culture medium.Results:In NR and MTT assays, Lamium album extracts did not affect cell viability (80% at 125 μg/ml concentration). Ethanol was the least toxic extract (cell viability over 88%) and expressed the most potent reactive oxygen species (ROS) scavenging action. It was 19.88 ± 0.87% higher than controls representing a reduction corresponding to 7.136 μg/ml of trolox. Heptane extract revealed no ROS scavenging activity. All extracts decreased NO production by cells. The most active extract was ethanol (8 μg/ml) which reduced NO level to 0.242 μM (75% decrease compared to control). Extracts influenced pro-inflammatory (IL-1, IL-6, TNF-α) and anti-inflammatory (IL-10) cytokines levels reducing all of them in general. The strongest reduction in tested cytokines level was observed by the heptane extract. On the other hand, the ethanol extract induced mainly TNF-α level in a concentration dependent manner.Conclusion:Selected Lamium album extracts influence human corneal epithelial cells. Generally, while not toxic, they modulate pro-inflammatory and anti-inflammatory cytokines levels, and decrease NO release by cells; moreover, ethanol and ethyl acetate extracts reduce ROS levels.

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HPTLC‐densitometric method of determination of oleanolic acid in the Lamii albi flos

Cited by 9 publications

References 16 publications

Histochemical and Phytochemical Analysis of Lamium album subsp. album L. Corolla: Essential Oil, Triterpenes, and Iridoids

Histochemical and Phytochemical Analysis of Lamium album subsp. album L. Corolla: Essential Oil, Triterpenes, and Iridoids

Determination of oleanolic acid and ursolic acid in Chinese medicinal plants using HPLC with PAH polymeric C18

The effect of Lamium album extract on cultivated human corneal epithelial cells (10.014 pRSV-T)

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