HPLC fractioning to study the synergy and antagonism of rue plant seeds alkaloid to inhibit topoisomerase II as antitumor

Kadhim, Naser Jawad; Aziz, Dhifaf Zeki; Hadi, Maysaa Adil

doi:10.18081/2333-5106/016-7/253-262

Cited by 3 publications

(6 citation statements)

References 10 publications

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“…A variety of phytochemicals present in crude extracts have either antagonistic or synergistic effects. Crude extract has a lower potential than standard treatment, which may be the result of the activity of trace compounds such as alkaloids that are commonly present in the ethanolic extract Evidence-Based Complementary and Alternative Medicine of EO fruits [62,63]. Furthermore, alkaloids have display antagonistic effects to phenolic compounds during antioxidant activity [64].…”

Section: Discussionmentioning

confidence: 99%

Ethanolic Fruit Extract of Emblica officinalis Suppresses Neuroinflammation in Microglia and Promotes Neurite Outgrowth in Neuro2a Cells

Phochantachinda

Chatchaisak

Temviriyanukul

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Inhibiting neuroinflammation and modulating neurite outgrowth could be a promising strategy to prevent neurological disorders. Emblica officinalis (EO) may be a potent agent against them. Although EO extract reportedly has anti-inflammatory properties in macrophages, there is limited knowledge about its neuroprotective activity by suppressing microglia-mediated proinflammatory cytokine production and inducing neurite outgrowth. The present study aimed to elucidate the effect of EO fruit extract on the lipopolysaccharide- (LPS-) induced neuroinflammation using microglial (BV2) and neuroblastoma (Neuro2a) cells. The results demonstrated that, in LPS-treated BV2 cells, EO fruit extract reduced nitric oxide, interleukin-6, and tumor necrotic factor-α production. It also enhanced the neurite length of Neuro2a cells, which was linked to the upregulation of TuJ1 and MAP2 expressions. In conclusion, these findings indicate that the ethanolic extract of EO fruits has promising neuroprotective potential to exhibit antineuroinflammation activity and accelerative effect on neurite outgrowth in vitro. Therefore, EO fruit extract can be considered a novel herbal medicine candidate for managing neuroinflammatory diseases.

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Section: Discussionmentioning

confidence: 99%

Ethanolic Fruit Extract of Emblica officinalis Suppresses Neuroinflammation in Microglia and Promotes Neurite Outgrowth in Neuro2a Cells

Phochantachinda

Chatchaisak

Temviriyanukul

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…The chloroform layer was collected and evaporated well to get alkaloids powder. The dry alkaloid extract placed in the refrigerator in an opaque glass container [29] .…”

Section: Preparation Of Custard Apple Plant Extractsmentioning

confidence: 99%

“…Run the agarose until the dye front (bromophenol blue) is about 4-6 cm down the gel. After running, stained with EB (0.5µg/ml) for 20min and then washed with distal water for 15 min before photo documentation (Bioneer) [29] . The activity of enzyme % calculate according to the following equation (Eq.…”

Section: Protocol For Topoisomerase II Unit Assay (Final Volume Of 10 Ul)mentioning

confidence: 99%

The Concentration Factor Effect Study of Etoposide Drug and Annona squamosa L. Seeds Extracts on Topoisomerase II Inhibition Model: Catalytic Inhibitory and Interfacial Poisons

2021

IJFMT

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Background: Topoisomerase II plays an important role in cell proliferation and DNA regulation. It is responsible for loosening the supercoiled form of DNA and for condensing chromosomes in eukaryotic cells. Custard apple (Annona squamosa L.) (Annonaceae) has been one of the main ingredients of ethnomedical preparation for the treatment of tumors in some countries, and has cytotoxic effects on cancerous cell lines. Aim: assessment of Qualitative Inhibition (Catalytic Inhibitor or Interfacial Poisons) and Quantitative (IC50%) of Custard apple Plant Seeds; Crude Extract (CCE) and Alkaloid Extract (CAE) of Human Toposomerase IIA (TOP2A) compared to etoposide (VP-16) as a controlled drug. Methods: TOP2A (0.5 unit) and pHOT1 DNA plasmid (62.5ng), DNA plasmid relaxation assay required for inhibition testing. Inhibitors, the seeds plant was extracted by 100% methanol for the preparation of crude with concentrations

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“…Alkaloid derivatives, harmine, and harmaline decrease or inhibit the growth of cancer cells by induction of apoptosis in a dose-dependent manner (Bernardo et al, 2012;Hamsa & Kuttan, 2011). In addition, they act as apoptotic inducers through through the creatine phosphokinase (CPK) effect of topoisomerase enzyme inhibition in the G1 phase of cell division (Kadhim, Aziz, & Hadi, 2016;Shu et al, 2019).…”

Section: The Aqueous and Ethanolic Extracts And Other Compounds Ofmentioning

confidence: 99%

“…Notably, Β-carboline-mediated inhibition of the DNA synthesis and DNA helix intercalate leading to inhibition of the DNAs topoisomerases-1 and 2 and the growth inhibition of cancer cells in mice was confirmed. In addition, the inhibitory effect on the DNA and RNA synthesis was exerted through the creatine phosphokinase (CPK) effect of topoisomerase enzyme inhibition in the G1 phase of cell division(Kadhim, Aziz, & Hadi, 2016;Shu et al, 2019).Garlic compounds such as diallyl sulfide (NAG-1 proapoptotic gene induction), methyl allyl trisulphide (inhibition of benzo-pyrene and lipoxygenase in cancer cells), S-methyl-1-systein sulphoxide, and methiin (cell cycle arrest) are associated with epithelial cancer cell apoptosis and cell cycle arrest(Kaowinn et al, 2018;Moosavi et al, 2016; G. Wang, Liu, Ye, Fu, & Zhang, 2016).…”

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confidence: 99%

Herbal medicine as an auspicious therapeutic approach for the eradication ofHelicobacter pyloriinfection: A concise review

Ghasemian

Fattahi

Mostafavi

et al. 2019

Journal Cellular Physiology

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Helicobacter pylori (H. pylori) causes gastric mucosa inflammation and gastric cancer mostly via several virulence factors. Induction of proinflammatory pathways plays a crucial role in chronic inflammation, gastric carcinoma, and H. pylori pathogenesis. Herbal medicines (HMs) are nontoxic, inexpensive, and mostly anti‐inflammatory reminding meticulous emphasis on the elimination of H. pylori and gastric cancer. Several HM has exerted paramount anti‐H. pylori traits. In addition, they exert anti‐inflammatory effects through several cellular circuits such as inhibition of 5′‐adenosine monophosphate‐activated protein kinase, nuclear factor‐κB, and activator protein‐1 pathway activation leading to the inhibition of proinflammatory cytokines (interleukin 1α [IL‐1α], IL‐1β, IL‐6, IL‐8, IL‐12, interferon γ, and tumor necrosis factor‐α) expression. Furthermore, they inhibit nitrous oxide release and COX‐2 and iNOS activity. The apoptosis induction in Th1 and Th17‐polarized lymphocytes and M2‐macrophagic polarization and STAT6 activation has also been exhibited. Thus, their exact consumable amount has not been revealed, and clinical trials are needed to achieve optimal concentration and their pharmacokinetics. In the aspect of bioavailability, solubility, absorption, and metabolism of herbal compounds, nanocarriers such as poly lactideco‐glycolide‐based loading and related formulations are helpful. Noticeably, combined therapies accompanied by probiotics can also be examined for better clearance of gastric mucosa. In addition, downregulation of inflammatory microRNAs (miRNAs) by HMs and upregulation of those anti‐inflammatory miRNAs is proposed to protect the gastric mucosa. Thus there is anticipation that in near future HM‐based formulations and proper delivery systems are possibly applicable against gastric cancer or other ailments because of H. pylori.

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HPLC fractioning to study the synergy and antagonism of rue plant seeds alkaloid to inhibit topoisomerase II as antitumor

Cited by 3 publications

References 10 publications

Ethanolic Fruit Extract of Emblica officinalis Suppresses Neuroinflammation in Microglia and Promotes Neurite Outgrowth in Neuro2a Cells

Ethanolic Fruit Extract of Emblica officinalis Suppresses Neuroinflammation in Microglia and Promotes Neurite Outgrowth in Neuro2a Cells

The Concentration Factor Effect Study of Etoposide Drug and Annona squamosa L. Seeds Extracts on Topoisomerase II Inhibition Model: Catalytic Inhibitory and Interfacial Poisons

Herbal medicine as an auspicious therapeutic approach for the eradication ofHelicobacter pyloriinfection: A concise review

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