HPLC–electrospray tandem mass spectrometry for simultaneous quantitation of eight plasma aminothiols: Application to studies of diabetic nephropathy

Jiang, Zhiting; Liang, Qiongling; Luo, Guangsheng; Hu, Ping; Li, Ping; Wang, Yiming

doi:10.1016/j.talanta.2008.08.031

Cited by 72 publications

(59 citation statements)

References 24 publications

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“…The integration windows for each of the scan methods are shown in Table 1, and the LODs of The absolute LODs for the thiols were in the sub-picomole range, and they were better than the LC-UV methods (11,12,33), most of the MS based detection methods (5,(34)(35)(36)(37)(38)(39), and the results via fluorescence measurement (40,41).…”

Section: Figures Of Merit For the Analytical Protocolmentioning

confidence: 99%

Determination of Thiols in Yeast by HPLC Coupled with LTQ-Orbitrap Mass Spectrometry after Derivatization with p-(Hydroxymercuri)benzoate

Rao

Xiang

Bramanti

et al. 2010

J. Agric. Food Chem.

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A liquid chromatography method with mass spectrometric detection has been developed for the simultaneous determination of six thiols in the sulfur metabolic pathway, including cysteine (Cys), homocysteine (HCys), glutathione (GSH), cysteinyl-glycine (Cys-Gly), γ-glutamyl-cysteine (Glu-Cys), and s-adenosyl-homocysteine (AdoHcy).Tris(2-carboxyethyl) phosphine (TCEP) was used as the reducing reagent and p-hydroxymercurybenzoate (PHMB) as the derivatization reagent. Thiols were extracted from three mg of yeast using water in an ultrasonic bath. The absolute detection limits for the compounds studied were in the sub-picomole range. It was found that AdoHcy, Cys, GSH, Cys-Gly, Glu-Cys, and very little HCys were present in the selenium enriched yeast sample studied, and GSH, Glu-Cys, very little AdoHcy, Cys, and Cys-Gly were present in three bakery yeasts.

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Section: Figures Of Merit For the Analytical Protocolmentioning

confidence: 99%

Determination of Thiols in Yeast by HPLC Coupled with LTQ-Orbitrap Mass Spectrometry after Derivatization with p-(Hydroxymercuri)benzoate

Rao

Xiang

Bramanti

et al. 2010

J. Agric. Food Chem.

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“…SAMe contents were determined by reverse phase-HPLC, as described previously (Jiang et al, 2009). The contents were quantified using an Dionex ultimate 3000 (USA) system.…”

Section: Quantification Of the Levels Of Same By Hplcmentioning

confidence: 99%

“…The concentrations of SAMe were determined using the equations of linear regression obtained from the calibration curves. Method validation including intra-day precision, inter-day precision, extraction recoveries and stabilities were analysed as described previously (Jiang et al, 2009;Krijt et al, 2009). …”

Section: Quantification Of the Levels Of Same By Hplcmentioning

confidence: 99%

5-Aza-2'-deoxycytidine Induces Hepatoma Cell Apoptosis via Enhancing Methionine Adenosyltransferase 1A Expression and Inducing S-Adenosylmethionine Production

Liu

Ren²,

Li³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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In hepatocellular cancer (HCC), lack of response to chemotherapy and radiation treatment can be caused by a loss of epigenetic modifications of cancer cells. Methionine adenosyltransferase 1A is inactivated in HCC and may be stimulated by an epigenetic change involving promoter hypermethylation. Therefore, drugs releasing epigenetic repression have been proposed to reverse this process. We studied the effect of the demethylating reagent 5-aza-2'-deoxycitidine (5-Aza-CdR) on MAT1A gene expression, DNA methylation and S-adenosylmethionine (SAMe) production in the HCC cell line Huh7. We found that MAT1A mRNA and protein expression were activated in Huh7 cells with the treatment of 5-Aza-CdR; the status of promoter hypermethylation was reversed. At the same time, MAT2A mRNA and protein expression was significantly reduced in Huh7 cells treated with 5-Aza-CdR, while SAMe production was significantly induced. However, 5-Aza-CdR showed no effects on MAT2A methylation. Furthermore, 5-Aza-CdR inhibited the growth of Huh7 cells and induced apoptosis and through down-regulation of Bcl-2, up-regulation of Bax and caspase-3. Our observations suggest that 5-AzaCdR exerts its anti-tumor effects in Huh7 cells through an epigenetic change involving increased expression of the methionine adenosyltransferase 1A gene and induction of S-adenosylmethionine production.

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“…Cellular and plasma levels of SAM and SAH correlate with each other and with total homocystein level [10]. Plasma levels of SAM and SAH are 60-90 and 20-30 nM, respectively [13], and therefore, highsensitivity methods are used for their analysis: HPLC and capillary electrophoresis (CE) with fl uorescent detection [2,12] and chloracetaldehyde derivatization as well as HPLC-MC [6,8,13]. This involves the use of sophisticated methods and/or is fraught with systematic errors due to derivatization reaction.…”

mentioning

confidence: 99%

S-Adenosylhomocysteine Assay in the Urine by Capillary Electrophoresis

et al. 2015

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HPLC–electrospray tandem mass spectrometry for simultaneous quantitation of eight plasma aminothiols: Application to studies of diabetic nephropathy

Cited by 72 publications

References 24 publications

Determination of Thiols in Yeast by HPLC Coupled with LTQ-Orbitrap Mass Spectrometry after Derivatization with p-(Hydroxymercuri)benzoate

Determination of Thiols in Yeast by HPLC Coupled with LTQ-Orbitrap Mass Spectrometry after Derivatization with p-(Hydroxymercuri)benzoate

5-Aza-2'-deoxycytidine Induces Hepatoma Cell Apoptosis via Enhancing Methionine Adenosyltransferase 1A Expression and Inducing S-Adenosylmethionine Production

S-Adenosylhomocysteine Assay in the Urine by Capillary Electrophoresis

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