HoxA10 Protein Regulates Transcription of Gene Encoding Fibroblast Growth Factor 2 (FGF2) in Myeloid Cells

Shah, Chirag; Bei, Ling; Wang, Hao; Platanias, Leonidas C.; Eklund, Elizabeth A.

doi:10.1074/jbc.m111.328401

Cited by 40 publications

(87 citation statements)

References 53 publications

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“…The sequence was compared with the published FGF2 5Ј-flank sequence (ENSEMBL database), and promoter fragments were subcloned into the pGL3-basic reporter vector (Promega) as described (26). Other constructs were generated with mutation of identified Hox binding sites in the FGF2 promoter.…”

Section: Methodsmentioning

confidence: 99%

“…We used chromatin immunoprecipitation-based screening techniques to identify HoxA10 target genes that might be relevant to leukemogenesis (21)(22)(23)(24)(25)(26). In these studies we identified the gene encoding fibroblast growth factor 2 (Fgf2, or basic Fgf) as a HoxA10 target gene (26).…”

mentioning

confidence: 99%

“…In these studies we identified the gene encoding fibroblast growth factor 2 (Fgf2, or basic Fgf) as a HoxA10 target gene (26). Fgf2 is produced by bone marrow stromal cells, hematopoietic progenitor cells, and mature phagocytes (27).…”

mentioning

confidence: 99%

“…Serine/threonine phosphorylation of ␤-catenin by glycogen synthase kinase 3␤ leads to ␤-catenin ubiquitination and degradation. Therefore, Fgf2-induced activation of phosphoinositol 3-kinase stabilizes ␤-catenin and enhances ␤-catenin activity (26).…”

mentioning

confidence: 99%

“…We previously found that autocrine production of Fgf2 contributed to cytokine hypersensitivity of HoxA10-overexpressing myeloid progenitor cells in a phosphoinositol 3-kinase and ␤-catenin-dependent manner (26). The possibility that Fgf2 contributed to the pathogenesis of 11q23-AML was not previously explored.…”

mentioning

confidence: 99%

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The Leukemia-associated Mll-Ell Oncoprotein Induces Fibroblast Growth Factor 2 (Fgf2)-dependent Cytokine Hypersensitivity in Myeloid Progenitor Cells

Shah

Bei

Wang

et al. 2013

Journal of Biological Chemistry

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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The Leukemia-associated Mll-Ell Oncoprotein Induces Fibroblast Growth Factor 2 (Fgf2)-dependent Cytokine Hypersensitivity in Myeloid Progenitor Cells

Shah

Bei

Wang

et al. 2013

Journal of Biological Chemistry

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Long noncoding RNA LINC00483/microRNA‐144 regulates radiosensitivity and epithelial–mesenchymal transition in lung adenocarcinoma by interacting with HOXA10

Yang

et al. 2019

Journal Cellular Physiology

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Lung adenocarcinoma (LAD) is the leading cause of cancer death worldwide. Long noncoding RNAs (lncRNAs) have been shown to play an important regulatory role in cancer biology, including that of LAD. The aim of this experiment was to explore the interaction of LINC00483, microRNA‐144 (miR‐144), and homeobox A10 (HOXA10), and their effects on radio sensitivity and epithelial–mesenchymal transition (EMT) of LAD. Initially, microarray analysis was used to screen out miRNAs and lncRNAs, as well as the differentially expressed genes related to LAD. Following the screening process, the targeting relationship of LINC00483, miR‐144, and that of miR‐144 and HOXA10 was determined. Following that, the expression of LINC00483, miR‐144, messenger RNA (mRNA), as well as protein expression of HOXA10, MMP‐2, MMP‐9, E‐cadherin, vimentin, and N‐cadherin that followed in cells was determined. Also, the effect of LINC00483 on cell migration and invasion ability, and cell tumorigenic ability was detected. LINC00483 and HOXA10 were found to be upregulated whereas miR‐144 was downregulated in LAD. Silencing of LINC00483 could competitively bind to miR‐144, thereby upregulating HOXA10. LINC00483 or HOXA10 silencing led to decreased HOXA10, MMP‐2, MMP‐9, vimentin, and N‐cadherin but elevated miR‐144 and E‐cadherin. Moreover, after being transfected with silenced LINC00483, the cell proliferation, migration, and invasion were inhibited with enhanced radiosensitivity. Consequently, the data of the study indicates that interference of LINC00483 weakens its competitive binding ability to miR‐144, thus reducing HOXA10 expression, and enhancing radiosensitivity in LAD.

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HOXA10 Promotes Cell Invasion and MMP-3 Expression Via TGFβ2-Mediated Activation of the p38 MAPK Pathway in Pancreatic Cancer Cells

et al. 2014

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HoxA10 Protein Regulates Transcription of Gene Encoding Fibroblast Growth Factor 2 (FGF2) in Myeloid Cells

Cited by 40 publications

References 53 publications

The Leukemia-associated Mll-Ell Oncoprotein Induces Fibroblast Growth Factor 2 (Fgf2)-dependent Cytokine Hypersensitivity in Myeloid Progenitor Cells

The Leukemia-associated Mll-Ell Oncoprotein Induces Fibroblast Growth Factor 2 (Fgf2)-dependent Cytokine Hypersensitivity in Myeloid Progenitor Cells

Long noncoding RNA LINC00483/microRNA‐144 regulates radiosensitivity and epithelial–mesenchymal transition in lung adenocarcinoma by interacting with HOXA10

HOXA10 Promotes Cell Invasion and MMP-3 Expression Via TGFβ2-Mediated Activation of the p38 MAPK Pathway in Pancreatic Cancer Cells

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