2013
DOI: 10.18632/aging.100536
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Abstract: Here we describe a carefully optimized method for the preparation of high quality RNA by flow sorting of formaldehyde fixed senescent cells immunostained for any intracellular antigen. Replicative cellular senescence is a phenomenon of irreversible growth arrest triggered by the accumulation of a discrete number of cell divisions. The underlying cause of senescence due to replicative exhaustion is telomere shortening. We document here a spontaneous and apparently stochastic process that continuously generates … Show more

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Cited by 9 publications
(9 citation statements)
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References 27 publications
(42 reference statements)
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“…One key effector of different modes of cell senescence is the p16-pRB pathway (Adams 2009;Kuilman et al 2010). Interestingly, our analysis shows that a set of genes whose upregulation coincides with upregulation of p16 in one model of senescence (Jeyapalan and Sedivy 2013) are quite differently regulated between OIS and RS in our study. This is consistent with the idea that even the p16-pRB effector pathway is networked differently between OIS and RS.…”
Section: Discussionmentioning
confidence: 50%
See 1 more Smart Citation
“…One key effector of different modes of cell senescence is the p16-pRB pathway (Adams 2009;Kuilman et al 2010). Interestingly, our analysis shows that a set of genes whose upregulation coincides with upregulation of p16 in one model of senescence (Jeyapalan and Sedivy 2013) are quite differently regulated between OIS and RS in our study. This is consistent with the idea that even the p16-pRB effector pathway is networked differently between OIS and RS.…”
Section: Discussionmentioning
confidence: 50%
“…a Heat map showing relative expression of p16-linked genes in RS. The list of p16-linked genes was based on genes whose expression is either upregulated or downregulated in p16-expressing hTERT-immortalized human fibroblasts that spontaneously upregulate p16 (Jeyapalan and Sedivy 2013). Genes exhibiting a fold change >1.5 (or <-1.5) and BH-FDR-adjusted p value <0.05 were considered differentially expressed.…”
Section: Discussionmentioning
confidence: 99%
“…GSEA was run using the java command line version of the preranked algorithm with 2000 permutations. To overlap compartment switching genes with molecular phenotypes characteristic of senescent cells, we used the following published senescence-associated gene annotations: the DDR response ( 37 ), cell proliferation ( 38 ), KEGG cell cycle, SASP ( 38 , 39 ), genes whose expression changes in cells that have up-regulated p16 ( 38 , 40 ), and genes involved in the regulation of chromatin ( 38 ).…”
Section: Methodsmentioning
confidence: 99%
“…112 Gerontologists are focused on these "typically" senescent cells. 113 Yet, these senescent cells are only a tip of the iceberg. Characterized by increased cellular functions (hyperfunctions), gerogenic cells are not necessarily resemble "in vitro senescent fibroblasts.…”
Section: From Gerontology To Medicinementioning
confidence: 99%