Hot electron-induced cathodic electrochemiluminescence of rhodamine B at disposable oxide-coated aluminum electrodes

Jiang, Qinghong; Spehar, Anna-Maria; Håkansson, Markus; Suomi, Johanna; Ala-Kleme, Timo; Kulmala, Sakari

doi:10.1016/j.electacta.2005.08.004

Cited by 30 publications

(14 citation statements)

References 49 publications

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“…The intensity of HECL is quite independent of the pH of solution, being rather constant at pH range 3-10 [13,17], but depends strongly on the thickness of the insulating layer. Highest HECL intensity on silicon and aluminum electrodes is typically observed at oxide thickness 2-5 nm [13].…”

Section: Hecl On the Monolayer Coated Electrodesmentioning

confidence: 92%

“…HECL measurements were carried out by using single photon counting with an instrumentation that consisted of a Perkin-Elmer MP 1993 photon counting module with yellow sensitive cathode and Nucleus MCS-II scaler card, similar to previously described system [17]. The HECL signal was filtered with an optical filter of bandwidth 550 ± 40 nm.…”

Section: Instrumentation and Methodsmentioning

confidence: 99%

“…Fig. 3 shows calibration curves obtained on oxide-film coated aluminum and silicon electrodes in the presence of 0.01 M NaN 3 as coreactant [17]. Azide ions were used as coreactants, because previous study has shown that they quench background electroluminescence while slightly enhancing HECL intensity of Rhodamine B [17].…”

Section: Hecl Of Tamra-labeled Dna On Aluminum and Silicon Oxide-coatmentioning

confidence: 99%

“…Many common photoluminescent and chemiluminescent luminophores have been detected this way, examples including luminol and its derivatives [10,11], Ru(bpy) 3 2+ [12,13], fluorescein and eosin [14], SYBR (R) Green I [15], coumarine dyes [16], Rhodamine B [17] and terbium chelates [18,19]. The most sensitive HECL luminophores so far are terbium labels, which can be detected down to femtomolar concentration [18,19].…”

Section: Introductionmentioning

confidence: 99%

“…TAMRA is widely used label in fluorescence [34] and fluorescence resonance energy transfer based assays [35]. We used TAMRA-labeled target DNA strands as the HECL luminophores, because it is a commercially available labeling reagent, and our recent study has shown that parent compound of TAMRA, Rhodamine B, can be detected down to subnanomolar level using HECL [17]. Fig.…”

Section: Introductionmentioning

confidence: 99%

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Heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence of a rhodamine label at oxide-coated aluminum and silicon electrodes

Spehar‐Délèze

Suomi²,

Jiang³

et al. 2006

Electrochimica Acta

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This paper describes a heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence (HECL) of a rhodamine label. Thin oxide-film coated aluminum and silicon electrodes were modified with an aminosilane layer and derivatized with short, 15-mer oligonucleotides via diisothiocyanate coupling. Target oligonucleotides were conjugated with tetramethylrhodamine (TAMRA) dye at their amino modified 5 end and hybridization was detected using HECL of TAMRA. Preliminary results indicate sensitivity down to picomolar level and low nonspecific adsorption. The sensitivity was better on oxide-coated silicon compared to oxide-coated aluminum electrodes and two-base pair mismatched hybrids were successfully discriminated. The experimental results presented here might be useful for the design of disposable electrochemiluminescent DNA biosensors.

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Section: Hecl On the Monolayer Coated Electrodesmentioning

confidence: 92%

Section: Instrumentation and Methodsmentioning

confidence: 99%

Section: Hecl Of Tamra-labeled Dna On Aluminum and Silicon Oxide-coatmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence of a rhodamine label at oxide-coated aluminum and silicon electrodes

Spehar‐Délèze

Suomi²,

Jiang³

et al. 2006

Electrochimica Acta

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Versatile Supramolecular Nanovalves Reconfigured for Light Activation

Nguyen

Leung

Liong

et al. 2007

Adv Funct Materials

207

142

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All autonomous machines share the same requirement—namely, they need some form of energy to perform their operations and nanovalves are no exception. Supramolecular nanovalves constructed from [2]pseudorotaxanes—behaving as dissociatable complexes attached to mesoporous silica which acts as a supporting platform and reservoir—rely on donor‐acceptor and hydrogen bonding interactions between the ring component and the linear component to control the ON and OFF states. The method of operation of these supramolecular nanovalves involves primarily the weakening of these interactions. The [2]pseudorotaxane [BHEEEN ⊂ CBPQT]4+ [BHEEEN ≡ 1,5‐bis[2‐(2‐(2‐hydroxyethoxy)ethoxy)ethoxy]naphthalene and CBPQT4+ ≡ cyclobis(paraquat‐p‐phenylene)], when this 1:1 complex is tethered on the surface of the mesoporous silica, constitutes the supramolecular nanovalves. The mesoporous silica is charged against a concentration gradient with luminescence probe molecules, e.g., tris(2,2′‐phenylpyridyl)iridium(III), Ir(ppy)3 (ppy = 2,2′‐phenylpyridyl), followed by addition of CBPQT·4Cl to form the tethered [2]pseudorotaxanes. This situation corresponds to the OFF state of the supramolecular nanovalves. Their ON state can be initiated by reducing the CBPQT4+ ring with NaCNBH3, thus weakening the complexation and causing dissociation of the CBPQT4+ ring away from the BHEEEN stalks on the mesoporous silica particles MCM‐41 to bring about ultimately the controlled release of the luminescence probe molecules from the mesoporous silica particles with an average diameter of 600 nm. This kind of functioning supramolecular system can be reconfigured further with built‐in photosensitizers, such as tethered 9‐anthracenecarboxylic acid and tethered [Ru(bpy)2(bpy(CH2OH)2)]2+ (bpy = 2,2′‐bipyridine). Upon irradiation with laser light of an appropriate wavelength, the excited photosensitizers transfer electrons to the near‐by CBPQT4+ rings, reducing them so that they dissociate away from the BHEEEN stalks on the surface of the mesoporous silica particles, leading subsequently to a controlled release of the luminescent probe molecules. This control can be expressed in both a regional and temporal manner by the use of light as the ON/OFF stimulus for the supramolecular nanovalves.

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Triple Phase Boundary Photovoltammetry: Resolving Rhodamine B Reactivity in 4‐(3‐Phenylpropyl)‐Pyridine Microdroplets

et al. 2010

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Rhodamine B base is employed as a photoactive redox system in a liquid | liquid | electrode triple phase boundary electrochemical experiment. Microdroplets of the water-immiscible 3-(4-phenylpropyl)-pyridine (PPP) containing rhodamine B are deposited onto a basal plane pyrolytic graphite electrode surface which is then immersed into an aqueous solution containing 0.5 M phosphate buffer solution (pH 12). In cyclic voltammetry experiments, it is shown that dark reduction of rhodamine B to leuco-rhodamine B in PPP occurs in two steps--both one electron processes--and the re-oxidation occurs in a single two-electron process. Voltammetric signals are consistent with Na(+) transfer (for the first reduction step) and proton transfer (for the second reduction step) coupled to the electron transfer. A proton-driven disproportionation reaction (with k(dis)=3 mol(-1) dm(3) s(-1) at pH 12) is observed for the one-electron reduced intermediate. In the presence of light, a new photocatalysed oxidation process for the two-electron conversion of leuco-rhodamine B to rhodamine B is observed. Photoexcitation of rhodamine B is shown to trigger an effective photo-comproportionation mechanism. Quantitative insights into the dark and photomechanisms are obtained by comparison of rhodamine B and rhodamine B octadecylester and by applying an approximate numerical simulation procedure based on DigiSim.

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Hot electron-induced cathodic electrochemiluminescence of rhodamine B at disposable oxide-coated aluminum electrodes

Cited by 30 publications

References 49 publications

Heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence of a rhodamine label at oxide-coated aluminum and silicon electrodes

Heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence of a rhodamine label at oxide-coated aluminum and silicon electrodes

Versatile Supramolecular Nanovalves Reconfigured for Light Activation

Triple Phase Boundary Photovoltammetry: Resolving Rhodamine B Reactivity in 4‐(3‐Phenylpropyl)‐Pyridine Microdroplets

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