Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes

Fairbairn, David J.; Cavallaro, Antonino S.; Bernard, Margaret J.; Mahalinga-Iyer, Janani; Graham, Michael W.; Botella, José Ramón

doi:10.1007/s00425-007-0588-x

Cited by 160 publications

(100 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Now the success in increasing of plant resistance to nematodes has been reached by genetic engineering and breeding methods (Bleve-Zacheo et al, 2007;Fairbairn et al, 2007;Fuller et al, 2008;Gheysen et al, 2006;Liao et al, 2003;Tsygankova, Andrusevich, Ya, Ponomarenko et al, 2013;Tsygankova, Yemets et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Impact of New Natural Biostimulants on Increasing Synthesis in Plant Cells of Small Regulatory si/miRNA With High Anti-Nematodic Activity

Tsygankova¹,

Iutynska²,

Galkin³

et al. 2013

IJB

View full text Add to dashboard Cite

Plant endoparasitic cyst nematode Heterodera schachtii Schmidt, gallic nematode Meloidogyne incognita and stem nematode Ditylenchus destructor damage various agricultural crops. The application of ecologically safe natural biostimulants with bioprotective properties is a newer approach for increasing plant resistance to parasitic nematodes. The molecular-genetic analysis of biostimulants action on plant genome is necessary for creation of new effective bioregulators for plant protection against phytopathogenic organisms. In our field and greenhouse experiments, we investigated the influence of new natural biostimulants Avercom and its derivatives on plant protection against nematodes Meloidogyne incognita and Ditylenchus destructor. Considerable increase of resistance to nematodes and productivity of cucumber and potato were observed for plants treated by biostimulant Avercom and its derivatives. Impact of biostimulants Radostim-super and Avercom on increase of resistance of sugar beet and cucumber sprouts to nematodes Heterodera schachtii and Meloidogyne incognita was studied in the laboratory conditions. Comparative analysis of morpho-physiological signs of control and experimental plants showed that plants treated by Radostim-super and Avercom were more viable and resistant to these nematodes as compared to control sprouts. In the molecular-genetic experiments, we studied the impact of these biostimulants on inducing synthesis of small regulatory si/miRNA, which plays key role in plant immune protection. Using method Dot-blot hybridization we studied degree of homology between si/miRNA with mRNA populations, isolated from plants untreated and treated with new natural biostimulants. We found considerable difference in the degree of homology (6-28%) between populations of mRNA and si/miRNA from nematode-infected plants that were either untreated or treated with biostimulants. We have also investigated silencing of translation of mRNA activity of si/miRNA in the wheat embryo cell-free system of protein synthesis. In these experiments, we found high inhibitory activity (38-65%) of si/miRNA from plants treated by biostimulants as compared to low inhibitory activity (15-20%) of si/miRNA from untreated plants. Obtained differences in the degree of homology between populations of mRNA and si/miRNA from untreated and treated with biostimulants plants, which were infected by nematode, and also the high inhibitory activity of si/miRNA from plants treated by biostimulants confirm that these biostimulants induce synthesis of anti-nematodic si/miRNA in plants, resulting in considerable increase of their resistance to these phytopathogens.Keywords: Heterodera schachtii, Meloidogyne іncognita, Ditylenchus destructor, natural biostimulants, anti-nematodic si/miRNA, the degree of homology between mRNA and si/miRNA, silencing activity of si/miRNA in the wheat embryo cell-free system of protein synthesis, plant resistance to nematodes www.ccsenet.org/ijb

show abstract

Section: Discussionmentioning

confidence: 99%

Impact of New Natural Biostimulants on Increasing Synthesis in Plant Cells of Small Regulatory si/miRNA With High Anti-Nematodic Activity

Tsygankova¹,

Iutynska²,

Galkin³

et al. 2013

IJB

View full text Add to dashboard Cite

show abstract

“…Host induced gene silencing (HIGS) is a relatively new approach for controlling plant pathogens that relies on RNA interference to target the expression of essential pathogen genes. This strategy has been used to target a wide range of pathogen types including insects (reviews by Baum et al 2007;Huvenne and Smagghe 2010), nematodes (reviewed by Huang et al 2006;Yadav et al 2006;Fairbairn et al 2007;Sindhu et al 2009), fungi (Nowara et al 2010;Tinoco et al 2010;Yin et al 2011;Zhang et al 2012;Koch et al 2013;Panwar et al 2013;Pliego et al 2013;Ghag et al 2014), parasitic weeds (Tomilov et al 2008), and oomycetes (Govindarajulu et al 2014;VegaArreguin et al 2014;Jahan et al 2015). Pathogen effectors essential for virulence or Bhousekeeping^genes necessary for normal pathogen growth are typically the targets for HIGS.…”

Section: Resistance To Biotic and Abiotic Stressesmentioning

confidence: 99%

Biotech Potatoes in the 21st Century: 20 Years Since the First Biotech Potato

et al. 2015

View full text Add to dashboard Cite

Potato is the world's most important vegetable crop, with nearly 400 million tons produced worldwide every year, lending to stability in food supply and socioeconomic impact. In general, potato is an intensively managed crop, requiring irrigation, fertilization, and frequent pesticide applications in order to obtain the highest yields possible. Important traits are easy to find in wild relatives of potato, but their introduction using traditional breeding can take 15-20 years. This is due to sexual incompatibility between some wild and cultivated species, a desire to remove undesirable wild species traits from adapted germplasm, and difficulty in identifying broadly applicable molecular markers. Fortunately, potato is amenable to propagation via tissue culture and it is relatively easy to introduce new traits using currently available biotech transformation techniques. For these reasons, potato is arguably the crop that can benefit most by modern biotechnology. The benefits of biotech potato, such as limited gene flow to conventionally grown crops and weedy relatives, the opportunity for significant productivity and nutritional quality gains, and reductions in production cost and environmental impact, have the potential to influence the marketability of newly developed varieties. In this review we will discuss current and past efforts to develop biotech potato varieties, traits that could be impacted, and the potential effects that biotech potato could have on the industry.Resumen La papa es el cultivo hortícola más importante en el mundo, con cerca de 400 millones de toneladas producidas a nivel mundial anualmente, acreditando la estabilidad en el suministro de alimentos e impacto socioeconómico. En general, la papa es un cultivo manejado intensivamente, que requiere riego, fertilización y aplicaciones frecuentes de plaguicidas para obtener los más altos rendimientos posibles. Los caracteres importantes son fáciles de encontrar en parientes silvestres de la papa, pero su introducción usando el mejoramiento tradicional puede llevar de 15 a 20 años. Esto es debido a la incompatibilidad sexual entre algunas especies silvestres y cultivadas, el deseo para eliminar características indeseables de las especies silvestres del germoplasma adaptado, y la dificultad en la identificación de marcadores moleculares aplicables ampliamente. Afortunadamente, la papa es receptiva a la propagación por cultivo de tejidos y es relativamente fácil la introducción de nuevos caracteres usando técnicas biotecnológicas de transformación actualmente disponibles. Por estas razones, la papa es probablemente el cultivo que se puede beneficiar mayormente por la biotecnología moderna. Los beneficios de la papa biotecnológica, como el flujo genético limitado a cultivos que se siembran convencionalmente y a los parientes como malezas, la oportunidad para productividad significativa y logros en calidad nutricional, y las reducciones en los costos de producción e impacto ambiental, tienen el potencial para influenciar la comercialización...

show abstract

“…In general, plant scientists, backed by results of modern comprehensive profiling of crop composition, point out that crops modified using GM techniques are less likely to have unintended changes than are conventionally bred crops [34]. With rather more advancement, plant biotechnologists are functioning to generate plants with geneticallyengineered resistance to these particular plant infections [35].…”

Section: Herbicide Tolerance and Disease Resistancementioning

confidence: 99%

Physiological and Biochemical Significance of Genetically Modified Foods: An Overview

Mishra¹

2013

TONUTRAJ

View full text Add to dashboard Cite

Biotechnological discoveries and inventions have been observed to improve food production qualitatively as well as quantitatively. In certain specific cases, the improvement in the quality and nutrition of foods by altering their composition were also monitored. However, the practice of biotechnology has also upraised concerns about its potential risks to the environment as well as human being. Genetic Engineering provides resources to host genes into plants via mechanisms, different in some respects from classical breeding. A number of genetically engineered variety of foods have been developed, which have become important nutraceuticals; most notably canola, cotton, maize and soybean, were developed employing this modern technology, and at present the traits introduced are herbicide and/or pest tolerance. Gene technology leads to increase the production in plants, as well as the elevation of resistance to pests, viruses, frost, etc. Gene transfer technology is employed to alter the physical and chemical composition with nutraceutical worth. The present review article is the compilation of various physiological and biochemical studies reflecting both positive and negative ecological concerns of genetically modified foods.

show abstract

Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes

Cited by 160 publications

References 38 publications

Impact of New Natural Biostimulants on Increasing Synthesis in Plant Cells of Small Regulatory si/miRNA With High Anti-Nematodic Activity

Impact of New Natural Biostimulants on Increasing Synthesis in Plant Cells of Small Regulatory si/miRNA With High Anti-Nematodic Activity

Biotech Potatoes in the 21st Century: 20 Years Since the First Biotech Potato

Physiological and Biochemical Significance of Genetically Modified Foods: An Overview

Contact Info

Product

Resources

About