Host-defense and trefoil factor family peptides in skin secretions of the Mawa clawed frog Xenopus boumbaensis (Pipidae)

Conlon, J. Michael; Mechkarska, Milena; Kolodziejek, Jolanta; Leprince, Jérôme; Coquet, Laurent; Jouenne, Thierry; Vaudry, Hubert; Nowotny, Norbert; King, Jay D.

doi:10.1016/j.peptides.2015.03.025

Cited by 8 publications

(4 citation statements)

References 35 publications

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“…In the specific case of the C1q domain containing protein, it could be due to its role as a pathogen recognition protein and its fast upregulation after an infection, with a return to physiological levels within 24 h 23 . The putative gastrointestinal growth factor xP4 is a member of the trefoil factor family (TFF), a group of molecules with a pivotal role in maintaining the surface integrity of mucous epithelia in vivo , explaining its up-regulation after the injury in control mussels; although the exact role of the TFF peptides is unknown they are known to have antiapoptotic effects and probably modulate inflammatory processes 36,37 a possible reason of its diverse behavior in control and infected samples. The other 3 annotated genes, always down regulated, were heat shock protein beta-1, associated with the acute phase response and found modulated after a tissue injury in M .…”

Section: Resultsmentioning

confidence: 99%

High individual variability in the transcriptomic response of Mediterranean mussels to Vibrio reveals the involvement of myticins in tissue injury

Rey-Campos

Moreira

Valenzuela-Muñoz

et al. 2019

Sci Rep

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Mediterranean mussels ( Mytilus galloprovincialis ) are sessile filter feeders that live in close contact with numerous marine microorganisms. As all invertebrates, they lack an adaptive immune response and how these animals are able to respond to a bacterial infection and discriminate it from their normal microbiome is difficult to understand. In this work, we conducted Illumina sequencing of the transcriptome of individual mussels before and after being infected with Vibrio splendidus . The control mussels were injected with filtered seawater. We demonstrate that a great variability exists among individual transcriptomes and that each animal showed an exclusive repertoire of genes not shared with other individuals. The regulated genes in both the control and infected mussels were also analyzed and, unexpectedly, the sampling before the injection was considered a stress stimulus strong enough to trigger and modulate the response in hemocytes, promoting cell migration and proliferation. We found a clear response against the injection of filtered seawater, suggesting a reaction against a tissue injury in which the myticins, the most expressed antimicrobial peptides in mussel, appeared significantly up regulated. Functional experiments with flow cytometry confirmed the transcriptomic results since a significant alteration of hemocyte structures and a decrease in the number of hemocytes positive for myticin C were found only after a Vibrio infection and not observed when mussels were bled before, generating a tissue injury. Therefore, we report the involvement of myticins in the response to a danger signal such as a simple injection in the adductor muscle.

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Section: Resultsmentioning

confidence: 99%

High individual variability in the transcriptomic response of Mediterranean mussels to Vibrio reveals the involvement of myticins in tissue injury

Rey-Campos

Moreira

Valenzuela-Muñoz

et al. 2019

Sci Rep

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“… a Color code: Hydrophilic amino acids, hydrophobic amino acids, and cysteine are marked in blue, black, and red, respectively. − …”

Section: General Characteristics Of Ampsmentioning

confidence: 99%

Peptide Dendrimer-Based Antibacterial Agents: Synthesis and Applications

Paul,

Verma,

Chen

2024

ACS Infect. Dis.

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Pathogenic bacteria cause the deaths of millions of people every year. With the development of antibiotics, hundreds and thousands of people’s lives have been saved. Nevertheless, bacteria can develop resistance to antibiotics, rendering them insensitive to antibiotics over time. Peptides containing specific amino acids can be used as antibacterial agents; however, they can be easily degraded by proteases in vivo. To address these issues, branched peptide dendrimers are now being considered as good antibacterial agents due to their high efficacy, resistance to protease degradation, and low cytotoxicity. The ease with which peptide dendrimers can be synthesized and modified makes them accessible for use in various biological and nonbiological fields. That is, peptide dendrimers hold a promising future as antibacterial agents with prolonged efficacy without bacterial resistance development. Their in vivo stability and multivalence allow them to effectively target multi-drug-resistant strains and prevent biofilm formation. Thus, it is interesting to have an overview of the development and applications of peptide dendrimers in antibacterial research, including the possibility of employing machine learning approaches for the design of AMPs and dendrimers. This review summarizes the synthesis and applications of peptide dendrimers as antibacterial agents. The challenges and perspectives of using peptide dendrimers as the antibacterial agents are also discussed.

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“…CPF-AM1 is a cationic AMP firstly isolated from X. amieti [110]. This peptide is capable of bacterial binding LPS and has activity against Gram-negative and Grampositive bacteria, primarily oral and respiratory pathogens, with advantages such as low hemolytic activity and lack of toxicity against fibroblast cells [109].…”

Section: Caerulein Precursor Fragment (Cpf)mentioning

confidence: 99%

Insights on Antimicrobial Peptides

2022

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She completed her post-doctoral studies in the United States and Japan. Dr. Enany was one of the first Arab scientists to apply bacterial proteomic techniques to reveal good markers for microbes spreading in the community. She has received many awards for her scientific contributions, including the 2021 International Science Council Award for Early Career Scientist in Africa, the 2019 Egyptian state encouragement prize for women in the field of health and pharmaceutical sciences, and the 2018 World Academy of Sciences Young Arab Scientist Prize in Medical Sciences. PrefaceMicroorganisms are ubiquitous and play an important but "bifacial" role in the existence of plants, animals, and humans. Some microorganisms are necessary for maintaining vital functions but may play a negative role and lead to infectious diseases and even death. Thus, all existing species must protect themselves from excess colonization of microorganisms that can cause infection and death. The immune system defends the organism against different types of infections. Antimicrobial peptides (AMPs) are an important part of the innate immune system of different organisms living in nature. Unfortunately, the immune system cannot always defeat the infection. Infectious diseases were an enormous problem in the past. Before the discovery of antibiotics, extracts of herbs and plants and chemical compounds were used as therapies for infectious diseases. Everything changed with the discovery of penicillin, which marked the start of the "antibiotic era." Antibiotics were determined to be "miracle drugs" and intensively used in medicine for the successful treatment of bacterial infection. However, this intensive usage in human and veterinary medicine, sometimes as a preventive step, has led to drug resistance.Microorganisms are good at adapting to different stress conditions and have different mechanisms of antibiotic resistance such as alteration of membrane permeability, enzymatic degradation of antibiotics, modification of bacterial proteins that are antimicrobial targets, and active efflux. Modern medicine is impossible without antibiotics, but conclusions of the "antibiotic era" aren't optimistic. Global antibiotic usage led to the development of superbugs, and the number of resistant bacteria and multidrug-resistant (MDR) bacteria is rapidly increasing. As such, we still have problems with infectious disease treatment despite an arsenal of antibiotics, many of which have become ineffective against modern (resistant) microorganisms.Another problem with modern antibacterial treatment is that we currently use antibiotics discovered more than three decades ago. A new antibiotic is necessary, but unfortunately, the development of new antibiotics is costly, takes years, and is an unprofitable process for pharmaceutical companies. This book presents a comprehensive overview of AMPs with potential for medical use. The AMPs described are isolated from different sources, such as ascidians, cyanobacteria, frogs, and more.Chapter by Rajesh and Vanathi describes t...

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Host-defense and trefoil factor family peptides in skin secretions of the Mawa clawed frog Xenopus boumbaensis (Pipidae)

Cited by 8 publications

References 35 publications

High individual variability in the transcriptomic response of Mediterranean mussels to Vibrio reveals the involvement of myticins in tissue injury

High individual variability in the transcriptomic response of Mediterranean mussels to Vibrio reveals the involvement of myticins in tissue injury

Peptide Dendrimer-Based Antibacterial Agents: Synthesis and Applications

Insights on Antimicrobial Peptides

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