Hormonal dynamics during salt stress responses of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea

Přerostová, Sylva; Dobrev, Petre I.; Gaudinová, Alena; Hošek, Petr; Soudek, Petr; Knirsch, Vojtech; Vaňková, Radomı́ra

doi:10.1016/j.plantsci.2017.07.020

Cited by 65 publications

(56 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Only the level of cis-zeatin riboside increased in roots after the stress (Table S1). This is in agreement with the results of several studies (e.g., Gajdosova et al, 2011;Prerostova et al, 2017), which showed that cis-zeatin and its riboside are up-regulated during abiotic stresses.…”

Section: Apices Leaves Rootssupporting

confidence: 93%

Heat Acclimation and Inhibition of Cytokinin Degradation Positively Affect Heat Stress Tolerance of Arabidopsis

et al. 2020

Self Cite

View full text Add to dashboard Cite

In order to pinpoint phytohormone changes associated with enhanced heat stress tolerance, the complex phytohormone profiles [cytokinins, auxin, abscisic acid (ABA), jasmonic acid (JA), salicylic acid and ethylene precursor 1-aminocyclopropane-1carboxylic acid (ACC)] were compared in Arabidopsis thaliana after direct heat shock (45°C, 3 h) and in heat-stressed pre-acclimated plants (1 h at 37°C followed by 2 h at optimal temperature 20°C). Organ-specific responses were followed in shoot apices, leaves, and roots immediately after heat shock and after 24-h recovery at 20°C. The stress strength was evaluated via membrane ion leakage and the activity of nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOX) and antioxidant enzymes [superoxide dismutases, guaiacol peroxidases (POD), catalases, ascorbate peroxidases (APX)]. Heat acclimation diminished negative effects of heat stress, especially in apices and roots, no significant differences being observed in leaves. Low NOX1-3 activities indicated diminished production of reactive oxygen species. Higher activity of APX, POD1, and the occurrence of POD3-4 reflected acclimation-stimulated readiness of the antioxidant system. Acclimation diminished heat shock-induced changes of ABA, JA, cytokinin, and auxin levels in apices. Excess of ABA catabolites suggested an early stress response. The strong up-regulation of ABA and ACC in roots indicated defense boost in roots of acclimated plants compared to the non-acclimated ones. To evaluate the possibility to enhance stress tolerance by cytokinin pool modulation, INCYDE-F, an inhibitor of cytokinin oxidase/dehydrogenase, was applied. As cytokinin effects on stress tolerance may depend on timing of their regulation, INCYDE was applied at several time-points. In combination with acclimation, INCYDE treatment had a slight positive effect on heat stress tolerance, mainly when applied after 2-h period of the optimal temperature. INCYDE increased contents of cytokinins trans-zeatin and cis-zeatin in roots and auxin in all tissues after heat shock. INCYDE also helped to suppress the content of ABA in leaves, and ethylene in apices and roots. INCYDE application to non-acclimated plants (applied before Frontiers in Plant Science | www.frontiersin.org or after heat shock) strengthened negative stress effects, probably by delaying of the repair processes. In conclusion, pre-treatment with moderately elevated temperature enhanced heat stress tolerance and accelerated recovery after stress. Inhibition of cytokinin degradation by INCYDE slightly improved recovery of acclimated plants.

show abstract

Section: Apices Leaves Rootssupporting

confidence: 93%

Heat Acclimation and Inhibition of Cytokinin Degradation Positively Affect Heat Stress Tolerance of Arabidopsis

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Importantly the highly active form t Z is significantly increased to nearly 200% the level of untreated plants and levels of cZ are even more highly increased. Although cZ is known to be a less active cytokinin form it has been previously connected to salt and other stress responses (Prerostova et al., ; Schäfer et al., ). Together this shows that cytokinin levels are increased in young tomato seedlings in response to salt stress.…”

Section: Discussionmentioning

confidence: 99%

Salt and oxidative stresses uniquely regulate tomato cytokinin levels and transcriptomic response

et al. 2018

View full text Add to dashboard Cite

Cytokinins are well‐known to be involved in processes responsible for plant growth and development. More recently, these hormones have begun to be associated with stress responses as well. However, it is unclear how changes in cytokinin biosynthesis, signaling, or transport relate to stress effects. This study examines in parallel how two different stresses, salt, and oxidative stress, affect changes in both cytokinin levels and whole plant transcriptome response. Solanum lycopersicum seedlings were given a short‐term (6 hr) exposure to either salt (150 mM NaCl) or oxidative (20 mM H2O2) stress and then examined to determine both changes in cytokinin levels and transcriptome. LC‐MS/MS was used to determine the levels of 22 different types of cytokinins in tomato plants including precursors, active, transported, and conjugated forms. When examining cytokinin levels we found that salt treatment caused an increase in both active and inactive cytokinin levels and oxidative stress caused a decrease in these levels. RNA‐sequencing analyses of these same stress‐treated tissues revealed 6,643 significantly differentially expressed genes (DEGs). Although many DEGs are similar between the two stresses, approximately one‐third of the DEGs in each treatment were unique to that stress. Several cytokinin‐related genes were among the DEGs. Examination of photosystem II efficiency revealed that cytokinins affect physiological response to stress in tomato, further validating the changes in cytokinin levels seen in planta.

show abstract

“…Major phytohormonal balances, such as ABA vs. brassinosteroids, ABA vs. cytokinins, ABA vs. Gibberellins, or SA vs. JA (jasmonic acid), are affected by climate change-related environmental stresses (Prerostova et al, 2017;Verma et al, 2016;Verslues, 2016;Zhou et al, 2014). These changes in hormonal balance lead to transcriptome and proteome reprogramming through networks of hormone signaling pathways (Prerostova et al, 2017;Verma et al, 2016;Verslues, 2016). Such hormonal changes are under upstream control of stress and environmental sensing and signaling, which regulate hormone metabolism (Verslues, 2016).…”

Section: Hormonal Status Signaling Under Climate Change Conditionsmentioning

confidence: 99%

“…Besides changes in metabolic status, climate change-related factors induce deep changes in all phytohormones and therefore of the hormonal status (Verma, Ravindran, & Kumar, 2016). Major phytohormonal balances, such as ABA vs. brassinosteroids, ABA vs. cytokinins, ABA vs. Gibberellins, or SA vs. JA (jasmonic acid), are affected by climate change-related environmental stresses (Prerostova et al, 2017;Verma et al, 2016;Verslues, 2016;Zhou et al, 2014). These changes in hormonal balance lead to transcriptome and proteome reprogramming through networks of hormone signaling pathways (Prerostova et al, 2017;Verma et al, 2016;Verslues, 2016).…”

Section: Hormonal Status Signaling Under Climate Change Conditionsmentioning

confidence: 99%

“…In the presence of 75 mM NaCl, Arabidopsis seedlings accumulate SA in root and shoot apices, whereas in the presence of 100 mM NaCl, SA accumulation is located in roots and leaves. Moreover, certain plant species show threshold responses, with global SA concentrations increasing up to a given NaCl exposure threshold, and then decreasing beyond such a threshold (Prerostova et al, 2017). Stress intensity can modulate acclimation processes through the extent of SA increase.…”

Section: Hormonal Status Signaling Under Climate Change Conditionsmentioning

confidence: 99%

See 1 more Smart Citation

Pivotal roles of environmental sensing and signaling mechanisms in plant responses to climate change

Bigot

Buges

Gilly

et al. 2018

Global Change Biology

View full text Add to dashboard Cite

Climate change reshapes the physiology and development of organisms through phenotypic plasticity, epigenetic modifications, and genetic adaptation. Under evolutionary pressures of the sessile lifestyle, plants possess efficient systems of phenotypic plasticity and acclimation to environmental conditions. Molecular analysis, especially through omics approaches, of these primary lines of environmental adjustment in the context of climate change has revealed the underlying biochemical and physiological mechanisms, thus characterizing the links between phenotypic plasticity and climate change responses. The efficiency of adaptive plasticity under climate change indeed depends on the realization of such biochemical and physiological mechanisms, but the importance of sensing and signaling mechanisms that can integrate perception of environmental cues and transduction into physiological responses is often overlooked. Recent progress opens the possibility of considering plant phenotypic plasticity and responses to climate change through the perspective of environmental sensing and signaling. This review aims to analyze present knowledge on plant sensing and signaling mechanisms and discuss how their structural and functional characteristics lead to resilience or hypersensitivity under conditions of climate change. Plant cells are endowed with arrays of environmental and stress sensors and with internal signals that act as molecular integrators of the multiple constraints of climate change, thus giving rise to potential mechanisms of climate change sensing. Moreover, mechanisms of stress-related information propagation lead to stress memory and acquired stress tolerance that could withstand different scenarios of modifications of stress frequency and intensity. However, optimal functioning of existing sensors, optimal integration of additive constraints and signals, or memory processes can be hampered by conflicting interferences between novel combinations and novel changes in intensity and duration of climate change-related factors. Analysis of these contrasted situations emphasizes the need for future research on the diversity and robustness of plant signaling mechanisms under climate change conditions.

show abstract

Hormonal dynamics during salt stress responses of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea

Cited by 65 publications

References 47 publications

Heat Acclimation and Inhibition of Cytokinin Degradation Positively Affect Heat Stress Tolerance of Arabidopsis

Heat Acclimation and Inhibition of Cytokinin Degradation Positively Affect Heat Stress Tolerance of Arabidopsis

Salt and oxidative stresses uniquely regulate tomato cytokinin levels and transcriptomic response

Pivotal roles of environmental sensing and signaling mechanisms in plant responses to climate change

Contact Info

Product

Resources

About