Horizontal Relaxing Incisions Limit False Positives in Thick Mohs Sections

Lin, Steven W.; Dorrian, James; Engasser, Holly; Deem, Anna; Endrizzi, Bart T.

doi:10.1111/dsu.12031

Cited by 6 publications

(13 citation statements)

References 3 publications

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“…To achieve this, both the deep and lateral margins need to be in one plane for cryo-sectioning. Methods that help this process include the use of relaxing incisions [ 19 ], the glass slide embedding technique [ 20 ], or cutting 100 µm spaced tissue sections through the specimen [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

“…The pre-processing of tissue was also effective at removing the majority of superficial blood. The investigated area could be further increased by performing multiple relaxing incisions on the epidermal side of stiffer specimens to allow better contact between the tissue and the cassette window and by implementing an automated, user-invariant method for washing/blotting of the tissue blocks [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

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Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma

Boitor

Wolf

Weesie

et al. 2021

Biomed. Opt. Express

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We present the first clinical integration of a prototype device based on integrated auto-fluorescence imaging and Raman spectroscopy (Fast Raman device) for intra-operative assessment of surgical margins during Mohs micrographic surgery of basal cell carcinoma (BCC). Fresh skin specimens from 112 patients were used to optimise the tissue pre-processing and the Fast Raman algorithms to enable an analysis of complete Mohs layers within 30 minutes. The optimisation allowed >95% of the resection surface area to be investigated (including the deep and epidermal margins). The Fast Raman device was then used to analyse skin layers excised from the most relevant anatomical sites (nose, temple, eyelid, cheek, forehead, eyebrow and lip) and to detect the three main types of BCC (nodular, superficial and infiltrative). These results suggest that the Fast Raman technique is a promising tool to provide an objective diagnosis “tumour clear yes/no” during Mohs surgery of BCC. This clinical integration study is a key step towards a larger scale diagnosis test accuracy study to reliably determine the sensitivity and specificity in a clinical setting.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma

Boitor

Wolf

Weesie

et al. 2021

Biomed. Opt. Express

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“…Decreased artifact was defined as decreased crush artifact during mounting, decreased freezing artifact for embedding medium, or decreased floaters when staining or marking borders of tumor margin. 37,38…”

Section: Decreased Artifactmentioning

confidence: 99%

“…In 47 studies, tissue conservation was assessed (77%). [8][9][10][11][12][13][14][15][16][18][19][20][21]25,26,[28][29][30][31]33,36,38,39,41, Authors of 13 studies defined conservation of tissue as the fewest number of sections to obtain a complete margin, 8,10,20,28,29,36,41,45,49,50,58,59,64 whereas others defined it as tissue conserved by minimizing the thickness or depth of an excised layer (n 5 9), [12][13][14][15]26,31,46,59,62 minimizing tissue manipulation (n 5 8), 11,18,21,25,…”

Section: Conservation Of Tissuementioning

confidence: 99%

Reported Outcome Measures in Mohs Micrographic Surgery in Studies With Defined Techniques for Embedding and Processing of Tissue: A Systematic Review

Govas,

Ha,

Wessel

et al. 2023

Dermatol Surg

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BACKGROUND Mohs micrographic surgery (MMS) is a technique that combines surgical excision and histologic evaluation to achieve higher cure rates for skin cancer than traditional surgical excision. Competing performance measures have fostered numerous histologic techniques for MMS. OBJECTIVE To analyze differences in primary outcomes in the published literature regarding the technique of tissue processing and embedding during the MMS process. METHODS A systematic review was performed of the published literature in MEDLINE, PubMed, Embase, and Cochrane library that included a description of the manipulation of tissue during the grossing and embedding steps of MMS. RESULTS Inclusion criteria were met by 61 articles. Of these studies, the cure/recurrence rate was assessed in 1 article (1.6%), tissue conservation was assessed in 47 (77%), time-saving was assessed in 35 (57%), cost-saving was assessed in 6 (10%), and decreased artifact were assessed in 20 (33%). CONCLUSION There is a lack of standardization for assessing clinical outcomes in the published literature regarding MMS process techniques. Cure is a critical outcome in studies comparing MMS processing methodologies.

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“…1,2 Previously reported methods that target curvatures include relaxing incisions and positioning samples using buttresses created from embedding medium. 1,3,4 A case involving serpentine complex contours of the ear is presented, and it highlights grossing strategies to separate each curve into a distinct piece.…”

mentioning

confidence: 99%

Mohs Surgery on the Complex Contours of the Ear Optimized With Coordinated Subdivision to Isolate Sections With Single Curves

et al. 2023

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Horizontal Relaxing Incisions Limit False Positives in Thick Mohs Sections

Cited by 6 publications

References 3 publications

Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma

Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma

Reported Outcome Measures in Mohs Micrographic Surgery in Studies With Defined Techniques for Embedding and Processing of Tissue: A Systematic Review

Mohs Surgery on the Complex Contours of the Ear Optimized With Coordinated Subdivision to Isolate Sections With Single Curves

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