Honey and olive oil as bio-friendly substitutes for formalin and xylene in routine histopathology

Muddana, Keerthi; Muppala, Jaya Nagendra Krishna; Dorankula, Shyam Prasad Reddy; Maloth, Aruna Kumari; Kulkarni, Pavan G; Thadudari, Deepsagar

doi:10.4103/ijdr.ijdr_246_16

Cited by 8 publications

(16 citation statements)

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“…Root resorption or root divergence may also be observed. [ 10 ] In the present case, a large multilocular radiolucency was seen with interrupted borders associated with horizontally impacted canine at the lower border of the mandible. Also root divergence was seen in all of the teeth associated with the lesion (31, 32, 34, 35, 41, 42).…”

Section: Discussionmentioning

confidence: 48%

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Ameloblastomatous calcifying cystic odontogenic tumour involving the mandibular anterior region: An unusual case report

Choudhary¹,

Gade

Jagtap

et al. 2023

J Oral Maxillofac Pathol

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Ameloblastomatous calcifying odontogenic cyst (COC) is a very rare histopathological variant of COC, which is an odontogenic cyst of the jaw. The term “calcifying odontogenic cyst” is not covered in the World Health Organisation (WHO) Classification of Tumors 2005 and was re-named as calcifying cystic odontogenic tumour (CCOT). There are only a few reports containing details on CCOT being associated with ameloblastoma. This variant has been classified as ameloblastomatous CCOT (type 3) as per the WHO 2005 classification. In this article, we reported an exceptional case of ameloblastomatous CCOT in a 15-year-old boy involving the mandibular anterior region, which is a rare combination for age and site of the lesion, along with an impacted tooth which is again an uncommon association.

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Section: Discussionmentioning

confidence: 48%

“…As per the literature, only 32 cases of CCOT associated with ameloblastoma have been reported, with very few reports having details about the clinical and radiographic features. [ 6 7 10 ] Association of this hybrid lesion with impacted canine in the present case makes it unique.…”

Section: Discussionmentioning

confidence: 83%

Ameloblastomatous calcifying cystic odontogenic tumour involving the mandibular anterior region: An unusual case report

Choudhary¹,

Gade

Jagtap

et al. 2023

J Oral Maxillofac Pathol

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“…Therefore, we need to find a substitute for xylene if we wanted to embed large tissues. Various xylene-substitutes had been commercially developed to avoid the most obvious disadvantages of xylene 16 , 18 – 20 , including esters, aromatic, naphthenic, higher aliphatic hydrocarbons, terpene-based extracts and chlorinated hydrocarbons. We tested three xylene substitutions and compared them with xylene.…”

Section: Resultsmentioning

confidence: 99%

Paraffin-embedding for large volume bio-tissue

Zhanmu

Yang

Gong

et al. 2020

Sci Rep

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Acquiring ultrahigh-resolution three-dimensional images of large-volume tissues non-human primate tissues was an enormous challenge. Given the preservation of structure and excellent sectioning property, formalin-fixed paraffin-embedding method had an enormous potential for threedimensional reconstruction of fine structures, based on the very thin histological sections and optical images. However, maintaining the structure uniformly in large-volume tissues was difficult during the complex processes. In this study, we presented a detailed protocol for the whole mouse, rat, rabbit brains, and even for the macaque hemisphere. The entire protocol took about 2-30 days to complete for a large sample, including fixation, dehydration, clearing, wax immersion and embedding. In addition, it could be applied to other species and organs, while the embedding processes depended on the size and the type of organs. This method had wide applicability to serve as a baseline for further technique development.

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“…Our histology laboratory followed a standard protocol for tissue processing, dehydrating, clearing, and staining samples as typically used and previously described (Clarke et al, 2007;Sun et al, 2009;Ghanaati et al, 2011). It is accepted that the xylene solution is routine histopathology since it clears the tissue and renders it transparent (Swamy et al, 2015) while removing the alcohol (Muddana et al, 2017). The histological slides were successful when the purple nucleus and pinkness cytoplasm were accepted.…”

Section: Discussionmentioning

confidence: 99%

A histological method for marine invertebrates

Kongthong

Charoenphon

Thaochan

et al. 2022

VIS

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This study aimed to increase knowledge about the structure and health assessment for evaluating histopathology as a biomarker of marine invertebrates, which must have high-quality histologic slides. Unfortunately, the accuracy of this method has been specifically modified for the marine invertebrates in Thailand; we demonstrated a modified method for permanent histological slides, including Xestospongia sp., Halobates hayanus, Amphibalanus amphitrite, and Alpheus sp. (n = 30) individual specimens for each species) from Libong island, Thailand, which was compared to the standard method. Optimization of tissue adapts and troubleshoots typical histological procedures, including sample collection, fixation with 10% Neutral Buffered Formalin at 2-4 °C for 24 hrs., decalcification, dehydration, clearing, embedding sectioning, and examining after staining under the modified procedures each time, which can be accomplished easily in six days. Compared to the standard method, this modified protocol showed the first report. It warranted high-quality slide scans, which are particularly ideal for the tissue, cell, and nuclei-specific features of marine invertebrates.

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Honey and olive oil as bio-friendly substitutes for formalin and xylene in routine histopathology

Abstract: The preservation of tissue by honey giving superior result when compared to that of formalin. Olive oil was found to be effective clearing agent compared to xylene.

Cited by 8 publications

References 0 publications

Ameloblastomatous calcifying cystic odontogenic tumour involving the mandibular anterior region: An unusual case report

Ameloblastomatous calcifying cystic odontogenic tumour involving the mandibular anterior region: An unusual case report

Paraffin-embedding for large volume bio-tissue

A histological method for marine invertebrates

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