2005
DOI: 10.1074/jbc.m409029200
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Abstract: We have defined the homotypic interactions of fibrillin-1 to obtain new insights into microfibril assembly. Dosedependent saturable high affinity binding was demonstrated between N-terminal fragments, between furin processed C-terminal fragments, and between these Nand C-terminal fragments. The N terminus also interacted with a downstream fragment. A post-furin cleavage site C-terminal sequence also interacted with the N terminus, with itself and with the furin-processed fragment. No other homotypic fibrillin-… Show more

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Cited by 72 publications
(113 citation statements)
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“…The assembly of fibrillins likely requires many steps leading from individual fibrillin molecules to the final macromolecular assemblies in tissues-a process that includes self-assembly (Trask et al, 1999;Lin et al, 2002;Marson et al, 2005;Hubmacher et al, 2008), disulfide and transglutaminase cross-link formation (Qian and Glanville, 1997;Reinhardt et al, 2000), fibril elongation and others mechanisms (for review see Tiedemann et al, 2004). Fibrillins require the presence of cells to multimerize, and mesenchymal cells such as fibroblasts or smooth muscle cells, which can be readily manipulated, are an ideal system to analyze fibrillin assembly on the molecular level.…”
Section: Discussionmentioning
confidence: 99%
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“…The assembly of fibrillins likely requires many steps leading from individual fibrillin molecules to the final macromolecular assemblies in tissues-a process that includes self-assembly (Trask et al, 1999;Lin et al, 2002;Marson et al, 2005;Hubmacher et al, 2008), disulfide and transglutaminase cross-link formation (Qian and Glanville, 1997;Reinhardt et al, 2000), fibril elongation and others mechanisms (for review see Tiedemann et al, 2004). Fibrillins require the presence of cells to multimerize, and mesenchymal cells such as fibroblasts or smooth muscle cells, which can be readily manipulated, are an ideal system to analyze fibrillin assembly on the molecular level.…”
Section: Discussionmentioning
confidence: 99%
“…In this process, cell-associated multimerization of the fibrillin-1 C-terminus appears to be an important determinant in generating high-affinity binding sites for the fibrillin-1 N-terminus (Hubmacher et al, 2008). In addition to linear N-C interactions, lateral homotypic interactions in different regions of the fibrillin-1 molecule may play a role in stabilizing initial multimers or the lateral associa-tions of individual microfibrils (Trask et al, 1999;Ashworth et al, 1999;Marson et al, 2005). Although glycosaminoglycans of the heparin/heparan sulfate family have an important, but as yet undefined role in microfibril formation (Tiedemann et al, 2001;Ritty et al, 2003), it is currently not known whether other components on the cell surface or in the extracellular matrix are involved in the multifacetted fibrillin assembly process.…”
Section: Introductionmentioning
confidence: 99%
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