Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells

Sinjab, Faris; Awuah, Dennis; Gibson, Graham M.; Padgett, Miles J.; Ghaemmaghami, Amir M.; Notingher, Ioan

doi:10.1364/oe.26.025211

Cited by 29 publications

(20 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The HOT-RMS instrument used here is described fully in reference [19]. Briefly, a CW Ti:Sapphire laser at 785 nm with approximately 1 W output power is expanded onto a phase-only liquid-crystal spatial light modulator (LC-SLM), which is used to structure the beam to form the optical traps at the sample, with real-time interactive update using RedTweezers software developed by Bowman et al [21] The beam is relayed via a telescope to the back aperture of a 60× 1.2 NA water immersion microscope objective (Olympus, UPLSAPO, Tokyo, Japan), placed on an incubated (Solent Scientific, Portsmouth, UK) inverted microscope (Olympus IX71).…”

Section: Hot-rms Instrumentmentioning

confidence: 99%

“…Briefly, a CW Ti:Sapphire laser at 785 nm with approximately 1 W output power is expanded onto a phase-only liquid-crystal spatial light modulator (LC-SLM), which is used to structure the beam to form the optical traps at the sample, with real-time interactive update using RedTweezers software developed by Bowman et al [21] The beam is relayed via a telescope to the back aperture of a 60× 1.2 NA water immersion microscope objective (Olympus, UPLSAPO, Tokyo, Japan), placed on an incubated (Solent Scientific, Portsmouth, UK) inverted microscope (Olympus IX71). Typically six separate focused laser spots were generated at the sample, with an estimated 50 mW per beam (using method outlined in reference [19]). Raman scattering excited from the same laser used for optical trapping is collected in the backward direction, passed through a dichroic filter and focused onto a digital micromirror device (DMD) by a 25 mm diameter, 200 mm lens (Thorlabs AC254-200-B, Newton, New Jersey), resulting in a magnification from sample to DMD plane of 200/18 = 11.1×.…”

Section: Hot-rms Instrumentmentioning

confidence: 99%

“…Raman scattering excited from the same laser used for optical trapping is collected in the backward direction, passed through a dichroic filter and focused onto a digital micromirror device (DMD) by a 25 mm diameter, 200 mm lens (Thorlabs AC254-200-B, Newton, New Jersey), resulting in a magnification from sample to DMD plane of 200/18 = 11.1×. [19] Raman spectra shown in all figures (except for Figure 1C, which shows raw data) have had a polynomial baseline subtracted. Circular DMD pinholes of roughly 110 μm diameter were utilized, corresponding to approximately 15 DMD mirror pixels across.…”

Section: Hot-rms Instrumentmentioning

confidence: 99%

“…[13][14][15][16] By enabling control over the cell-cell interaction, optical trapping has enabled investigation of the early events in intercellular biological processes, such as phagocytosis and synapse formation. [19,20] HOT-RMS has potential for studying interaction between parasites and host cells noninvasively, and observing the molecular specific changes of isotope labels from the very earliest stages. [18] Recently, an integrated HOT and RMS system was demonstrated to be powerful for measurement and manipulation of live cells and organisms.…”

Section: Introductionmentioning

confidence: 99%

“…[18] Recently, an integrated HOT and RMS system was demonstrated to be powerful for measurement and manipulation of live cells and organisms. [19,20] HOT-RMS has potential for studying interaction between parasites and host cells noninvasively, and observing the molecular specific changes of isotope labels from the very earliest stages.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Induction and measurement of the early stage of a host‐parasite interaction using a combined optical trapping and Raman microspectroscopy system

Sinjab

Elsheikha

Dooley

et al. 2019

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Hot-rms Instrumentmentioning

confidence: 99%

Section: Hot-rms Instrumentmentioning

confidence: 99%

Section: Hot-rms Instrumentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Induction and measurement of the early stage of a host‐parasite interaction using a combined optical trapping and Raman microspectroscopy system

Sinjab

Elsheikha

Dooley

et al. 2019

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

show abstract

Novel Plasmonic Nanocavities for Optical Trapping‐Assisted Biosensing Applications

Koya

Cunha

Guo

et al. 2020

Advanced Optical Materials

View full text Add to dashboard Cite

However, due to the intrinsic low optical response of small objects, there has been a clear trade-off between the size of a material and its response to light. [5] Recently, plasmonic nanostructures have emerged as leading platforms to enhance the weak optical signals of low dimensional materials including quantum dots (QDs), [6] small molecules, [7,8] and 2D monolayers. [9] The plasmonic enhancement of linear and nonlinear optical processes capitalizes on the near-and far-field properties of metallic (e.g., gold and silver) nanostructures. [10] One of the defining features of plasmonic nanostructures is their potential to confine light into deep subwavelength volumes, which has opened a new door to trap and manipulate dielectric, metallic, and biological nano-objects. [11] Moreover, metallic nanostructures are characterized by their capability to amplify the intensity of optical fields by orders of magnitude. The enhancement of local field intensity is attributed to the resonance of plasmon polaritons arising from the coupling of external electromagnetic fields to the collective oscillations of the conduction electrons. [12] A small perturbation (or change in the refractive index) of the near field zone of plasmonic nanostructures leads to significant shift in the plasmon polariton resonance wavelength, which has important implications for surface-enhanced sensing and spectroscopic applications. [13,14] Thus, for the ad-hoc enhancement of optical Plasmonic nanocavities have proved to confine electromagnetic fields into deep subwavelength volumes, implying their potentials for enhanced optical trapping and sensing of nanoparticles. In this review, the fundamentals and performances of various plasmonic nanocavity geometries are explored with specific emphasis on trapping and detection of small molecules and single nanoparticles. These applications capitalize on the local field intensity, which in turn depends on the size of plasmonic nanocavities. Indeed, properly designed structures provide significant local field intensity and deep trapping potential, leading to manipulation of nano-objects with low laser power. The relationship between optical trappinginduced resonance shift and potential energy of plasmonic nanocavity can be analytically expressed in terms of the intercavity field intensity. Within this framework, recent experimental works on trapping and sensing of single nanoparticles and small molecules with plasmonic nanotweezers are discussed. Furthermore, significant consideration is given to conjugation of optical tweezers with Raman spectroscopy, with the aim of developing innovative biosensors. These devices, which take the advantages of plasmonic nanocavities, will be capable of trapping and detecting nanoparticles at the single molecule level.

show abstract

Investigating the feasibility of spatially offset Raman spectroscopy for in‐vivo monitoring of bone healing in rat calvarial defect models

Dooley

McLaren

Rose

et al. 2020

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

A wide range of biomaterials and tissue-engineered scaffolds are being investigated to support and stimulate bone healing in animal models. Using phantoms and rat cadavers, we investigated the feasibility of using spatially offset Raman spectroscopy (SORS) to monitor changes in collagen concentration at levels similar to those expected to occur in vivo during bone regeneration (0-0.84 g/cm 3). A partial least squares (PLS) regression model was developed to quantify collagen concentration in plugs consisting of mixtures or collagen and hydroxyapatite (predictive power of ±0.16 g/cm 3). The PLS model was then applied on SORS spectra acquired from rat cadavers after implanting the collagen: hydroxyapatite plugs in drilled skull defects. The PLS model successfully predicting the profile of collagen concentration, but with an increased predictive error of ±0.30 g/cm 3. These results demonstrate the potential of SORS to quantify collagen concentrations, in the range relevant to those occurring during new bone formation.

show abstract

Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells

Cited by 29 publications

References 42 publications

Induction and measurement of the early stage of a host‐parasite interaction using a combined optical trapping and Raman microspectroscopy system

Induction and measurement of the early stage of a host‐parasite interaction using a combined optical trapping and Raman microspectroscopy system

Novel Plasmonic Nanocavities for Optical Trapping‐Assisted Biosensing Applications

Investigating the feasibility of spatially offset Raman spectroscopy for in‐vivo monitoring of bone healing in rat calvarial defect models

Contact Info

Product

Resources

About