Holographic 3D imaging – methods and applications

Svoboda, Jakub; Škereň, Marek; Květoň, Milan; Fiala, Pavel

doi:10.1088/1742-6596/415/1/012051

Cited by 8 publications

(9 citation statements)

References 7 publications

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“…Indeed, despite an optical field consists of amplitude and phase distributions, all detectors record intensity only. By using the information incorporated in the acquired hologram, a discrete version of the complex optical wavefront diffused by the sample object is numerically reconstructed at the plane of acquisition [38][39][40][42][43][44][45][46][47].…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, Raman spectroscopy can be easily combined with complementary optical approaches, such as holographic microscopy [7,8,36]. Based on the refractive index difference between the cell and the surrounding medium, holographic microscopy analyses the phase of the light transmitted by the sample and allows 3D quantitative sample image reconstruction [37][38][39][40][41][42]. Compared with the established morphological approaches used in biology such as fluorescence microscopy or more sophisticated techniques for instance atomic force microscopy (AFM), holographic imaging has three potential advantages: (i) The entire volume information can be acquired in one shot, avoiding mechanical movements [4,37,38,43].…”

Section: Introductionmentioning

confidence: 99%

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Combined Raman Spectroscopy and Digital Holographic Microscopy for Sperm Cell Quality Analysis

Angelis

Managò

Ferrara

et al. 2017

Journal of Spectroscopy

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The diagnosis of male infertility is vastly complex. To date, morphology, motility, and concentration have been used as key parameters to establish the sperm normality and achieve pregnancy both in natural and in assisted fecundation. However, spermatozoa from infertile men could present a variety of alterations, such as DNA fragmentation, alterations of chromatin structure, and aneuploidy, which have been demonstrated to decrease reproductive capacity of men. Therefore, the ability to see detailed relationships between morphology and physiology in selected spermatozoa with submicrometric resolution in a nondestructive and noninvasive way and within a functional correlated context could be extremely important for the intracytoplasmic sperm injection procedure. In this review, we describe label-free optical spectroscopy and imaging techniques, based on the combination of Raman spectroscopy/imaging with holographic imaging, which are able to noninvasively measure the (bio)chemistry and morphology of sperm cells. We discuss the benefits and limitation of the proposed photonic techniques, with particular emphasis on applications in detection/characterization of sperm cell morphological defects and photodamage, and the identification/sorting of X- and Y-bearing bovine spermatozoa.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Combined Raman Spectroscopy and Digital Holographic Microscopy for Sperm Cell Quality Analysis

Angelis

Managò

Ferrara

et al. 2017

Journal of Spectroscopy

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“…Holograms are widely used in a variety of fields including imaging [1,2,3], displays [4,5], lithography [6,7] and optical manipulation [8,9,10]. Computergenerated holograms are made possible by devices known as spatial light modulators (SLMs) that allow either the amplitude or phase of light incident on the device to be independently controlled and a diffraction pattern to be created.…”

Section: Introductionmentioning

confidence: 99%

Improving performance of single-pass real-time holographic projection

Christopher

Mouthaan

Bheemireddy

et al. 2020

Optics Communications

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This work describes a novel approach to time-multiplexed holographic projection on binary phase devices. Unlike other time-multiplexed algorithms where each frame is the inverse transform of independently modified target images, Single-Transform Time-Multiplexed (STTM) hologram generation produces multiple sub-frames from a single inverse transform. Uniformly spacing complex rotations on the diffraction field then allows the emulation of devices containing 2 N modulation levels on binary devices by using N sub-frames. In comparison to One-Step Phase Retrieval (OSPR), STTM produces lower mean squared error for up to N = 5 than the equivalent number of OSPR sub-frames with a generation time of 1 /N of the equivalent OSPR frame. A mathematical justification of the STTM approach is presented and a hybrid approach is introduced allowing STTM to be used in conjunction with OSPR in order to combine performance benefits.

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“…Computer generated holography (CGH) works in reverse, taking a target geometry and attempting to find an appropriate aperture modulation function that can reproduce it. Best known in display applications, CGH sees widespread application in a range of applications from lithography [7,8] and optical manipulation [9,10,11] to imaging [12,13,14] and displays [15,16].…”

Section: Introductionmentioning

confidence: 99%

Sympathetic quantisation - A new approach to hologram quantisation

Christopher

Mouthaan

Soliman

et al. 2020

Optics Communications

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Spatial light modulators can typically only modulate the phase or the amplitude of an incident wavefront, with only a limited number of discrete values available. This is often accounted for in computer-generated holography algorithms by setting hologram pixel values to the nearest achievable value during what is known as quantisation. Sympathetic quantisation is an alternative to this nearest-neighbour approach that takes into account the underlying diffraction relationships in order to obtain a significantly improved post-quantisation performance. The concept of sympathetic quantisation is introduced in this paper and a simple implementation, soft sympathetic quantisation, is presented which is shown to improve mean squared error and structural similarity index error metrics by 50% for the considered case of single-transform algorithms.

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Holographic 3D imaging – methods and applications

Cited by 8 publications

References 7 publications

Combined Raman Spectroscopy and Digital Holographic Microscopy for Sperm Cell Quality Analysis

Combined Raman Spectroscopy and Digital Holographic Microscopy for Sperm Cell Quality Analysis

Improving performance of single-pass real-time holographic projection

Sympathetic quantisation - A new approach to hologram quantisation

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