HLA-A, B and DRB1 allele and haplotype frequencies in volunteer bone marrow donors from the north of Parana State

Bardi, Marlene Silva; Jarduli, Luciana Ribeiro; Jorge, Adylson Justino; Camargo, Rossana Batista Oliveira Godoy; Carneiro, F; Gelinski, Jair Roberto; Silva, Roseclei Assunção Feliciano; Lavado, Edson Lopes

doi:10.5581/1516-8484.20120010

Cited by 28 publications

(27 citation statements)

References 16 publications

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“…Data from Curitiba population showed a predominance of a Caucasian sample, as well as observed at North of Paraná and Rio Grande do Sul. The HLA allelic and haplotypes frequencies were related between these populations with a similar immigration history .…”

Section: Methodsmentioning

confidence: 84%

HLA‐F polymorphisms in a Euro‐Brazilian population from Southern Brazil

et al. 2014

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HLA-F is a non-classical major histocompatibility complex (MHC) gene. It codes class Ib MHC molecules with restricted distribution and less nucleotide variations than MHC class Ia genes. Of the 22 alleles registered on the IMGT database only four alleles encode for proteins that differ in their primary structure. To estimate genotype and allele frequencies, this study targeted on known protein coding regions of the HLA-F gene. Genotyping was performed by Sequence Base Typing (SBT). The sample was composed by 199-unrelated bone marrow donors from the Brazilian Bone Marrow Donor Registry (REDOME), Euro-Brazilians, from Southern Brazil. About 1673 bp were analyzed. The most frequent allele was HLA-F*01:01 (87.19%), followed by HLA-F*01:03 (12.31%), HLA-F*01:02 (0.25%) and HLA-F*01:04 (0.25%). Significant linkage disequilibrium (LD) was verified between HLA-F and HLA classes I and II alleles. This is the first study regarding HLA-F polymorphisms in a Euro-Brazilian population contributing to the Southern Brazilian genetic characterization.

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Section: Methodsmentioning

confidence: 84%

HLA‐F polymorphisms in a Euro‐Brazilian population from Southern Brazil

et al. 2014

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“…Polymorphism of HLA-A, -B, and -DRB1 in Brazil was deeply analyzed based on serologic or low-resolution genotyping volunteer donors enrolled in REDOME (Brazilian Registry of Bone Marrow Donor). [53][54][55][56] However, few studies describe the diversity of many HLA loci at high resolution in the same population. Population studies are important for evolutionary and anthropological studies.…”

Section: Discussionmentioning

confidence: 99%

High‐resolution characterization of 12 classical and non‐classical HLA loci in Southern Brazilians

Castro

Issler

Gelmini

et al. 2019

HLA

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The human leukocyte antigen (HLA) are the most polymorphic genes in the human genome. Because of their importance for antigen recognition, HLA molecules play a central role in host defense and graft rejection upon transplantation. The aim of this study was to characterize allelic diversity of the classical HLA genes HLA‐A, ‐B, ‐C, ‐DRA, ‐DRB1, ‐DQA1, ‐DQB1, ‐DPA1, ‐DPB1, and the non‐classical class I genes HLA‐E, ‐F and ‐G at high‐resolution for a population of predominantly European ancestry from Curitiba, Brazil. Genotyping of 108 individuals was performed by next‐generation sequencing on the MiSeq platform and also by Sanger sequencing. The genotype distributions of all loci were in accordance with Hardy‐Weinberg equilibrium (P > 0.05) and a total of 202 HLA variants at second field resolution were observed for the 12 loci. The strongest linkage disequilibrium (r2 = 1.0, P < 10−5) was observed for the following pairs of alleles: HLA‐B*42:01:01 ~ HLA‐DRB1*03:02:01; HLA‐B*14:02:01 ~ HLA‐C*08:02:01; B*42:01:01 ~ HLA‐C*17:01:01; HLA‐DRB1*03:01:01 ~ HLA‐DQB1*02:01:01 ~ DRB1*03:01:01 ~ HLA‐DQB1*02:01:01; DRB1*13:01:01~ HLA‐DQB1*06:03:01 and HLA‐DRB1*09:01:02 ~ HLA‐DQA1*03:02. This is the first study to characterize all 12 HLA genes at high resolution in a single population. On the basis of the allelic frequencies of worldwide populations and principal component analysis, we confirmed the similarity of the study population to European and other Euro‐descendant populations.

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“…HLA‐A*02 and A*03 variants were more frequent in all groups, but no significant differences were observed between groups. In this study, the distribution of these alleles was similar to the results of other population studies carried out in Brazil, such as in Parana (Ruiz et al ., ; Bardi et al ., ), Sao Paulo (Salvadori et al ., ) and populations from north‐east of Brazil (Nigam et al ., ; Carvalho et al ., ). When compared with REDOME (), these allele variants had a similar distribution for each region, with a little difference in the frequencies presented in this study.…”

Section: Discussionmentioning

confidence: 99%

Allele and haplotype frequencies of HLA‐A, B, C, DRB1 and DQB1 genes in polytransfused patients in ethnically diverse populations from Brazil

Rodrigues

Macedo

Bruder

et al. 2015

Int J Immunogenetics

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The red blood transfusion is a practice often used in patients with haematological and oncological diseases. However, the investigation of human leucocyte antigen (HLA) system frequency in these individuals is of great importance because multiple transfusions may lead to HLA alloimmunization. Brazil is a country that was colonized by many other ethnicities, leading to a mixed ethnicity and regionalized population. In view of the importance of HLA typing in these patients, the aim of this study was to investigate the allele and haplotype frequencies from polytransfused patients from three different regions from Brazil. HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1 genotyping of 366 patients was performed by PCR-SSO, based on the Luminex technology (One Lambda(®) ), and the anti-HLA class I and class II antibodies were analysed using LabScreen Single Antigen Antibody Detection (One Lambda, Inc.). Allele and haplotype frequencies of polytransfused patients of three regions from Brazil were obtained using the Arlequin program. The most frequent allele frequencies observed were HLA-A*02, A*03, B*15, B*35, B*51, C*07, C*04, C*03, DRB1*13, DRB1*11, DRB1*07, DRB1*03, DRB1*01, DQB1*03, DQB1*02, DQB1*06 and DQB1*05. There were differences between the groups for allele variants HLA-B*57 (between Group 1 and Group 2) and HLA-C*12 (between Group 1 and Group 3). The most frequent haplotypes found in the sample were HLA-A*01B*08DRB1*03, DRBI*07DQB1*02, DRB1*01DQB1*05, DRB1*13DQB1*06 and A*02B*35. HLA class I and II antibodies were detected in 77.9% and 63.9% patients, respectively, while the both alloantibodies were detected in 62 (50.9%) patients. In conclusion, the HLA typing for polytransfused patients in each region has a great importance, as seen in this study; individuals from different regions from Brazil have HLA distribution not completely homogeneous.

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HLA-A, B and DRB1 allele and haplotype frequencies in volunteer bone marrow donors from the north of Parana State

Cited by 28 publications

References 16 publications

HLA‐F polymorphisms in a Euro‐Brazilian population from Southern Brazil

HLA‐F polymorphisms in a Euro‐Brazilian population from Southern Brazil

High‐resolution characterization of 12 classical and non‐classical HLA loci in Southern Brazilians

Allele and haplotype frequencies of HLA‐A, B, C, DRB1 and DQB1 genes in polytransfused patients in ethnically diverse populations from Brazil

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