HL-1 cells: A cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte

Claycomb, William C.; Lanson, Nicholas A.; Stallworth, Beverly S.; Egeland, Daniel B.; Delcarpio, Joseph B.; Bahinski, Anthony; Izzo, Nicholas J.

doi:10.1073/pnas.95.6.2979

Cited by 1,354 publications

(1,246 citation statements)

References 24 publications

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“…Our results indicate that the fibronectin-derived cell adhesion ligand RGDSP has a significant, dosedependent influence on HL-1 cardiomyocyte viability within 3-D PEG hydrogels. This result is consistent with previous 2-dimensional culture studies of HL-1 cardiomyocytes, in which fibronectin was required for cell growth while maintaining differentiated function [47]. Furthermore, the influence of RGDSP is more pronounced within degradable PEG hydrogels when compared to non-degradable PEG hydrogels, particularly at early time points.…”

Section: Discussionsupporting

confidence: 82%

An adaptable hydrogel array format for 3-dimensional cell culture and analysis

et al. 2008

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Hydrogels have been commonly used as model systems for 3-dimensional (3-D) cell biology, as they have material properties that resemble natural extracellular matrices (ECMs), and their cellinteractive properties can be readily adapted in order to address a particular hypothesis. Natural and synthetic hydrogels have been used to gain fundamental insights into virtually all aspects of cell behavior, including cell adhesion, migration, and differentiated function. However, cell responses to complex 3-D environments are difficult to adequately explore due to the large number of variables that must be controlled simultaneously. Here we describe an adaptable, automated approach for 3-D cell culture within hydrogel arrays. Our initial results demonstrate that the hydrogel network chemistry (both natural and synthetic), cell type, cell density, cell adhesion ligand density, and degradability within each array spot can be systematically varied to screen for environments that promote cell viability in a 3-D context. In a testbed application we then demonstrate that a hydrogel array format can be used to identify environments that promote viability of HL-1 cardiomyocytes, a cell line that has not been cultured previously in 3-D hydrogel matrices. Results demonstrate that the fibronectin-derived cell adhesion ligand RGDSP improves HL-1 viability in a dose-dependent manner, and that the effect of RGDSP is particularly pronounced in degrading hydrogel arrays. Importantly, in the presence of 70µM RGDSP, HL-1 cardiomyocyte viability does not decrease even after 7 days of culture in PEG hydrogels. Taken together, our results indicate that the adaptable, array-based format developed in this study may be useful as an enhanced throughput platform for 3-D culture of a variety of cell types.

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Section: Discussionsupporting

confidence: 82%

An adaptable hydrogel array format for 3-dimensional cell culture and analysis

et al. 2008

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“…That possibility was examined in two sublines of RAW cells over-expressing functional NRAMP1 (RAW 37) or non-functional antisense NRAMP1 (RAW 21). 15,16 RAW 37 cells exposed to high NTBI sera showed higher levels of endosomal CALG than either WT or RAW 21 cells following 3 h incubation with CALG supplemented thalassemia major sera ( Figure 6A). However, the fluorescence increase exerted by SIH, which reveals endosomal CALG-Fe complexes, showed Mechanism of iron overload by native plasma NTBI haematologica | 2012; 97 (5) 675 Figure 3.…”

Section: Ntbi Uptake and The Status Of Iron In Thalassemia Major Seramentioning

confidence: 99%

“…[13][14][15] We also used cultured HL-1 cardiac muscle cells derived from the AT-1 mouse atrial cardiomyocyte tumor lineage that continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype (generously provided by Dr WC Claycomb, LSU, New Orleans, USA). 16 …”

Section: Cell Culturementioning

confidence: 99%

The role of endocytic pathways in cellular uptake of plasma non-transferrin iron

Sohn¹,

Ghoti²,

Breuer³

et al. 2011

Haematologica

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“…HL-1 cells, a cardiac muscle cell line from the AT-1 mouse atrial cardiomyocyte tumor lineage, were a gift from William C. Claycomb, Ph.D.; Professor of Biochemistry and Molecular Biology LSU Health Sciences Center; New Orleans) [50]. HL-1 cells maintain the ability to contract and retain differentiated cardiac morphological, biochemical, and electrophysiological properties [50].…”

Section: Cell Isolation and Culturementioning

confidence: 99%

“…HL-1 cells maintain the ability to contract and retain differentiated cardiac morphological, biochemical, and electrophysiological properties [50].…”

Section: Cell Isolation and Culturementioning

confidence: 99%