Histopathological Evaluation of Zebrafish (Danio rerio) Intestinal Tissue After Imidacloprid Exposure

Abstract

The use of pesticides has been increasing day by day to increase productivity in agriculture. Pesticides used in the agricultural area mix into the aquatic ecosystem through rains and groundwater etc. and it threatens the life of aquatic organisms. Nowadays, one of the most used pesticide group is neonicotinoids. Imidacloprid is the most well known in this group. In this study, it was aimed to observe the histopathological effects of imidacloprid in zebrafish intestinal tissue. Adult zebrafish individuals were administered imidacloprid in concentrations of 9.5, 19, and 38 mg.L -1 for 5 days. When tissues are examined under light microscopy, deterioration of villi morphology, degeneration in brush border structure of epithelial cells, hyperplasia in Goblet cells, and enterocytes due to inflammation, enlargement, and disintegration in lamina propria were detected. Also, hypertrophy of the smooth muscle cells and thickening in the muscularis externa layer were observed. Dysplasia was detected in the small intestine tissues in samples belonging to the highest concentration group.

2,4-D Herbicide-Induced Hepatotoxicity: Unveiling Disrupted Liver Functions and Associated Biomarkers

“…Due to its high water solubility, the herbicide is able to reach conducting vessels through plants leaves and roots, thus spreading throughout the plant, causing abnormal tissue growth and other deleterious effects that ultimately lead to plant death [ 5 , 6 , 7 , 8 ]. Due to its high efficiency and low-cost, 2,4-D is globally used as a pre- and post-emergence herbicide in crops such as rice, coffee, sugarcane, corn, and soybeans [ 6 , 9 , 10 ].…”