Histopathological Changes of Organs (Lungs, Liver, Kidney, and Brain) After Using Two Types of AgiCoat and Acticoat Nanosilver Dressings on Deep Second-Degree Burn in Rat

Abstract

Prevention of infections is a very important issue in treating the burn wounds. The nanosilver dressings have many promising advantages, but absorption of silver ions and its adverse effects to the body were always a question. The aim of this study was to compare Silver serum levels and acute toxic effects of nanosilver on histopathology of organs (lungs, liver, kidney, spleen, and brain) in two types of AgiCoat and Acticoat (nanosilver) dressings on second-degree deep burn in rat. This is an experimental study conducted in our animal laboratory. We divided 24 Sprague–Dawley male rats weighing 300 to 350 randomly into two groups. After anesthesia, a second deep-degree burn was made over dorsal skins of rats by standard method. For group A, Agicoat and, for group B, Acticoat dressings were used. The dressings were changed every 3 days with AgiCoat and Acticoat, respectively. After 14 days, we got blood samples and tissue samples taken from heart, liver, kidneys, spleen, lungs, and brain and a sample from dorsal skin of the rat for histopathological examinations. The results showed that the levels of serum silver in both groups were significantly higher than the standard level (1.22 part per million (PM); AgiCoat, P = .017; Acticoat, P = .000), but there was no significant difference between the groups (P = .551). Examination of the relationship between the level of serum silver and histopathological changes in liver showed that hepatotoxicity of AgiCoat was higher compared with Acticoat and the difference was significant (P = .002). There were no pathological changes in brain, kidneys, spleen, heart, and lungs. Wound healing was faster in Acticoat group. The nanosilver dressings can cause toxicity in liver but not in kidney, brain, spleen, heart, and lungs. Liver pathology and hepatotoxicity were more prominent in AgiCoat group. Wound healing was faster in Acticoat group.

Analysis of the Curative Effect and Influencing Factors of Collagen Sponge Combined with Autologous Skin Graft in the Treatment of Deep Burn Patients

“…Clinically, the main cause of death of burn patients is often the infection caused by a large number of necrotic tissues attached to the wound surface. Early surgical methods are used to remove the dead burn necrotic tissues, that is, escharectomy, combined with skin grafting to seal the open wound surface [ 3 , 4 ]. With the improvement of people's living standards and the progress of science and technology, the treatment of burn patients is not limited to saving lives.…”

Effects of functional poly(ethylene terephthalate) nanofibers modified with sericin-capped silver nanoparticles on histopathological changes in parenchymal organs and oxidative stress in a rat burn wound model

“…In another experimental study, the heart, liver, kidneys, spleen, lungs, and brain were evaluated histopathologically after 14 days in a rat burn model. Although the level of hepatotoxicity due to silver particles and Agicoat was higher, no pathological findings were identified in the brain, kidneys, spleen, heart, or lungs (26).…”

Hyaluronic Acid-Silver Nanocomposites and Their Biomedical Applications: A Review

“…Recent studies point out to the cytotoxicity of silver, among others, to keratinocytes [ 21 ], mononuclear leucocytes [ 93 ] and macrophages [ 94 ]. In vivo experiments show that nanosilver accumulates in tissues of laboratory rats resulting in toxic damages of their livers; the changes in other organs have not been, however, observed [ 95 ]. The cytotoxicity results mainly from the oxidative stress induced by Ag NPs, but in the case of nanoparticles larger than 10 nm also from the release of the Ag + ions.…”