Histopathological and ultrastructural effects of delta-endotoxins of Bacillus thuringiensis serovar israelensis in the midgut of Simulium pertinax larvae (Diptera, Simuliidae)

Cavados, Clara F.G.; Majerowicz, Selma; Chaves, Jeane Q.; Araújo-Coutinho, Carlos J.P.C.; Rabinovitch, Leon

doi:10.1590/s0074-02762004000500006

Cited by 28 publications

(22 citation statements)

References 24 publications

(28 reference statements)

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“…In our investigation, we obtain the same results. Cavados et al (2004) revealed, in these study of Simulium pertinax larvae treated with Bacillus thuringiensis Serovar israelensis, a serious damage of the epithelial columnar cells. The most characteristic effects were midgut columnar cells vacuolization, microvilli damages, epithelium cell contents passing into Ndione et al 2853 the midgut lumen and finally the cell death.…”

Section: Discussionmentioning

confidence: 79%

Toxic effects of neem products (Azadirachta indica A. Juss) on Aedes aegypti Linnaeus 1762 larvae

Ndione¹,

Faye²,

Ndiaye³

et al. 2007

Afr. J. Biotechnol.

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Treatment and comparative analysis of the properties of aqueous extracts of seed kernel of Azadirachta indica A. Juss (neem) was carried out on Aedes aegypti larvae. The aim of this work was to evaluate lethal effects of neem products (1% Suneem, formulated neem oil and neem powder) on A. aegypti larvae. Assays showed that A. indica was toxic to larvae of A. aegypti. For 1% Suneem, 1% formulated neem oil and neem powder, the lethal concentrations and lethal time at 50% (LC 50 and LT 50 ) for A. aegypti were 2 and 8 mg/l after 24 h and 3 mg/l after 120 h, respectively. Assays showed that Suneem and Formulated neem oil were more toxic to A. aegypti than Neem powder. Both products of the neem (A. indica, A. juss) have a remarkable influence on the development of A. aegypti larvae, causing an inhibition of nymphs and adults emergency. The Histopathological results revealed a serious damage on the epithelial columnar cells, a perturbation of alimentary flow, slightly hypertrophied cells, a beginning of vacuolisation on apical level, and a bursting of some cells in posterior part of the gut. However, nuclei, adipose tissue and muscles seem to keep normal appearance.

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Section: Discussionmentioning

confidence: 79%

Toxic effects of neem products (Azadirachta indica A. Juss) on Aedes aegypti Linnaeus 1762 larvae

Ndione¹,

Faye²,

Ndiaye³

et al. 2007

Afr. J. Biotechnol.

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“…Dysfunctions of these organelles can be critical since they lead to an increase of reactive oxygen species that cause damage to major cellular components (2). Such changes in mitochondrial morphology (mitochondrial swelling and inner matrix destruction) have previously been reported for the action of B. thuringiensis toxins on midgut epithelia of mosquitoes and lepidopteran larvae (4,6,16,18,23). The ultrastructural effects of Cry48Aa/Cry49Aa thus appear to be a combination of those of Bin and three-domain Cry toxins.…”

Section: Discussionmentioning

confidence: 83%

Cytopathological Effects of Bacillus sphaericus Cry48Aa/Cry49Aa Toxin on Binary Toxin-Susceptible and -Resistant Culex quinquefasciatus Larvae

Melo

Jones

Berry

et al. 2009

Appl Environ Microbiol

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The Cry48Aa/Cry49Aa mosquitocidal two-component toxin was recently characterized from Bacillus sphaericus strain IAB59 and is uniquely composed of a three-domain Cry protein toxin (Cry48Aa) and a binary (Bin) toxin-like protein (Cry49Aa). Its mode of action has not been elucidated, but a remarkable feature of this protein is the high toxicity against species from the Culex complex, besides its capacity to overcome Culex resistance to the Bin toxin, the major insecticidal factor in B. sphaericus-based larvicides. The goal of this work was to investigate the ultrastructural effects

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“…The bacterial toxin causes several types of damage to gut epithelial cells, such as cytoplasm vacuolization, microvillus alteration, increase of the secretory cell activity and extrusion of the cell vesicles with intracytoplasmic material inside, and fi nally, cell death (Karch & Coz 1983, Percy & Fast 1983, Cavados et al 2004.…”

mentioning

confidence: 99%

Occurrence of apocrine secretion in the larval gut epithelial cells of Aedes aegypti L., Anopheles albitarsis Lynch-Arribálzaga and Culex quinquefasciatus say (Diptera: Culicidae): a defense strategy against infection by Bacillus sphaericus Neide?

Oliveira¹,

Tadei²,

Abdalla³

2009

Neotrop. entomol.

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Ocorrência de Secreção Apócrina nas Células Epiteliais do Intestino de Larvas de Aedes aegypti L.,Anopheles albitarsis Lynch-Arribálzaga e Culex quinquefasciatus Say (Diptera: Culicidae): um Mecanismo de Defesa contra Infecção Causada por Bacillus sphaericus Neide?RESUMO -Observou-se hipertrofi a das células do epitélio intestinal de Aedes aegypti (L.), Anopheles albitarsis (Lynch-Arribálzaga) e Culex quinquefasciatus (Say) devido ao aumento da atividade secretora intracelular como primeira reação às toxinas das linhagens 2362 e S1116 de Bacillus sphaericus (Neide). Após a hipertrofi a epitelial, formaram-se vesículas na porção apical das células, as quais eram compostas de fragmentos de membrana plasmática contendo material de natureza desconhecida em seu interior, caracterizando um tipo de secreção apócrina. A via de contaminação das larvas dos mosquitos por essas bactérias é pelo intestino, através da alimentação. Dependendo da espécie de Culicidade e da linhagem bacteriana utilizada, a hipertrofi a do epitélio ocorreu entre 5 a 15 min após a exposição das larvas ao meio contaminado. O segundo aspecto observado no processo de contaminação foi o aumento da atividade de secreção apócrina. As vesículas basófi las que se desprendiam das células permaneciam entre a membrana peritrófi ca e o lúmen intestinal. Observou-se atividade secretora tanto no grupo controle como no experimental, porém muito mais intensa no grupo experimental. Os Culicidae estudados apresentaram diferenças marcantes nas respostas às toxinas das bactérias utilizadas, sendo C. quinquefasciatus a espécie mais suscetível. As diferenças de reações das células em relação às toxinas produzidas pelas duas linhagens bacterianas são discutidas. PALAVRAS-CHAVE: Mosquito, controle biológico, virulênciaABSTRACT -Cell hypertrophy was the fi rst reaction of the gut epithelial cells of Aedes aegypti (L.), Anopheles albitarsis (Lynch-Arribálzaga) and Culex quinquefasciatus (Say) to the toxins of strains 2362 and S1116 of Bacillus sphaericus, as cells had an increase of intracellular secretory activity. Soon after the cell hypertrophy developed, vesicles were formed at the cell apical portion, which detached with the plasma membrane, characterizing a type of apocrine secretion like. The fi rst pathway of contamination of the mosquito larvae by these bacteria is through the gut, by feeding. Depending on the species of Culicidae and on the bacterial strain used, the hypertrophy of the gut epithelium occurred between 5 and 15 min after exposure to the contaminated environment. The second aspect observed after hypertrophy was the increase in apocrine secretion. The basophilic vesicles that detached from the cells remained between the peritrophic membrane and the gut lumen, such vesicles were fi lled with material of unknown nature. The gut posterior region showed secretory activity in both control and treated larvae, being much more intense in bacteria-exposed larvae. There were remarkable differences in the epithelial cell reaction according to the toxins of the ...

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Histopathological and ultrastructural effects of delta-endotoxins of Bacillus thuringiensis serovar israelensis in the midgut of Simulium pertinax larvae (Diptera, Simuliidae)

Cited by 28 publications

References 24 publications

Toxic effects of neem products (Azadirachta indica A. Juss) on Aedes aegypti Linnaeus 1762 larvae

Toxic effects of neem products (Azadirachta indica A. Juss) on Aedes aegypti Linnaeus 1762 larvae

Cytopathological Effects of Bacillus sphaericus Cry48Aa/Cry49Aa Toxin on Binary Toxin-Susceptible and -Resistant Culex quinquefasciatus Larvae

Occurrence of apocrine secretion in the larval gut epithelial cells of Aedes aegypti L., Anopheles albitarsis Lynch-Arribálzaga and Culex quinquefasciatus say (Diptera: Culicidae): a defense strategy against infection by Bacillus sphaericus Neide?

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