Histopathological and molecular effects of microplastics in Eisenia andrei Bouché

Rodríguez-Seijo, Andrés; Lourenço, Joana; Rocha-Santos, Teresa; Costa, João da; Duarte, Armando C.; Vala, Helena; Pereira, Ruth

doi:10.1016/j.envpol.2016.09.092

Cited by 468 publications

(166 citation statements)

References 31 publications

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“…Sublethal negative responses such as growth reduction were observed after the exposure of earthworms to 150 lm microplastics in their food (Lwanga et al, 2016). Such effects might be partially explained by histological damage and changes in the gene expression associated with microplastic exposure (Rodriguez-Seijo et al, 2017). Moreover, microplastics could act as vector of toxic Zn to earthworms under environmental conditions due to a higher adsorption of this metal to high density polyethylene microplastic (Hodson et al, 2017).…”

Section: The Need For Accurate An D Precise Quantification Of Micromentioning

confidence: 99%

Microplastics as an emerging threat to terrestrial ecosystems

Machado

Kloas

Zarfl

et al. 2018

Global Change Biology

1,531

537

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Microplastics (plastics <5 mm, including nanoplastics which are <0.1 μm) originate from the fragmentation of large plastic litter or from direct environmental emission. Their potential impacts in terrestrial ecosystems remain largely unexplored despite numerous reported effects on marine organisms. Most plastics arriving in the oceans were produced, used, and often disposed on land. Hence, it is within terrestrial systems that microplastics might first interact with biota eliciting ecologically relevant impacts. This article introduces the pervasive microplastic contamination as a potential agent of global change in terrestrial systems, highlights the physical and chemical nature of the respective observed effects, and discusses the broad toxicity of nanoplastics derived from plastic breakdown. Making relevant links to the fate of microplastics in aquatic continental systems, we here present new insights into the mechanisms of impacts on terrestrial geochemistry, the biophysical environment, and ecotoxicology. Broad changes in continental environments are possible even in particle-rich habitats such as soils. Furthermore, there is a growing body of evidence indicating that microplastics interact with terrestrial organisms that mediate essential ecosystem services and functions, such as soil dwelling invertebrates, terrestrial fungi, and plant-pollinators. Therefore, research is needed to clarify the terrestrial fate and effects of microplastics. We suggest that due to the widespread presence, environmental persistence, and various interactions with continental biota, microplastic pollution might represent an emerging global change threat to terrestrial ecosystems.

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Section: The Need For Accurate An D Precise Quantification Of Micromentioning

confidence: 99%

Microplastics as an emerging threat to terrestrial ecosystems

Machado

Kloas

Zarfl

et al. 2018

Global Change Biology

1,531

537

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“…Numerous studies testi ed the plastic particles in CPCP are the primary contributors to the aquatic plastic pollution and demonstrated the MPs exposure stimulates adverse effects in numerous marine and some terrestrial organisms. For example, altered growth and reproduction in rotifer, amphipod, and copepod [17,18,19], altered cellular functions in blue mussel and sea bass [20,21], reduced feeding activity in lugworm [22], brosis, congestion and in ammatory in ltration in earthworms [23] and activation of the immune response in mouse [24]. Therefore, the plastic particles are recognized as the second most poisonous agent affecting the environment and ecology during the second UN Environmental Conference in 2015 [25].…”

Section: Introductionmentioning

confidence: 99%

Prospects on the nano-plastic particles internalization and induction of cellular response in human keratinocytes

Gopinath

Twayana

Ravanan

et al. 2020

Preprint

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Background: Today cosmetic usage becomes customary in both sexes to improve their appearance and increase societal visibility. In this study, we have isolated nano-sized plastic particles ranging between 30 to 300 nm from the commercial face-scrubs and investigated its effects on keratinocytes. Results: Initially, nano-plastics adsorbed protein molecules and formed protein corona, thereby mimicked as protein aggregates, which then triggered the macropinocytosis activity. As a result, corrosion and degradation of plastic particles were observed. Concurrently, nano-plastics concentration-dependent cytotoxic, cytostatic, and cytoprotective activities were found in the keratinocytes. Additionally, a single dose of nano-plastics exposure for 48hrs resulted in the ROS mediated down-regulation of cell growth and proliferation inhibition followed by autophagy, finally, premature aging in HaCaT cells in 24 and 72 hrs of post-internalization, respectively. Conclusion: At the outset, this work provides insights into the nano-plastics concentration-dependent regulatory, cytoprotective, and cytotoxic effects in HaCaT cells. Further studies required to identify the detailed mechanisms of NPs toxicity on cells and the cytoprotective effect in cells at the molecular level.

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“…For instance, under high MP exposure, mortality level of the earthworm Lumbricus terrestris increased, growth inhibited, and biomass reduced, whereas even at higher concentrations no effect on reproduction was found (Huerta Lwanga et al, 2016). In comparison, Rodriguez-Seijo et al (2017) observed no adverse effects on survival, reproduction, or body weight of the earthworm Eisenia andrei, but did find gut damage and histological alterations including congestion and fibrosis. Under MP exposure, the earthworm Eisenia fetida showed only marginal effects, with the anti-oxidase system negatively affected only at artificially high concentrations (Wang et al, 2019), while the isopod Porcellio scaber was not affected by MP at all (Jemec Kokalj et al, 2018).…”

Section: Discussionmentioning

confidence: 93%

“…Early studies on earthworms under MP exposure indicated that some biological functions could be inhibited (Huerta Lwanga et al, 2016;Cao et al, 2017). One study documented histopathological damage, including congestion fibrosis and inflammatory infiltrates in earthworms in response to MP exposure, although no biological functions were affected (Rodriguez-Seijo et al, 2017). Wang et al (2019) found oxidative stress in earthworms in response to artificially high MP exposure only.…”

Section: Introductionmentioning

confidence: 99%

Microplastics Effects on Reproduction and Body Length of the Soil-Dwelling Nematode Caenorhabditis elegans

Schöpfer

Menzel

Schnepf

et al. 2020

Front. Environ. Sci.

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Microplastics (MP) are pervasive in the environment. There is ample evidence of negative MP effects on biota in aquatic ecosystems, though little is known about MP effects in terrestrial ecosystems. Given numerous entry routes of MP into soils, soil organisms are likely to be exposed to MP. We compared potential toxicological effects of MP from (i) low-density polyethylene (LDPE) (mean diameter ± standard deviation: 57 ± 40 µm) and (ii) a blend of biodegradable polymers polylactide (PLA) and poly(butylene adipateco-terephthalate) (PBAT) (40 ± 31 µm) on the reproduction and body length of the soil-dwelling bacterivorous nematode Caenorhabditis elegans. Feed suspensions without (control) or with MP (treatments) at concentrations of 1, 10, and 100 mg MP L −1 were prepared and nematodes were exposed to those suspensions on agar plates until completion of their reproductive phase (∼6 days). Using Nile red-stained PLA/PBAT MP particles and fluorescence microscopy, we demonstrated the ingestion of MP by C. elegans into pharynges and intestines. Under MP exposure, nematodes had fewer offspring (up to 22.9%) compared to nematodes in the control group. This decline was independent on the plastic type. We detected a tendency toward greater decreases in offspring at higher concentrations. Despite hints of negative effects on nematode body length under MP exposure, we could not derive a consistent pattern. We conclude that in MP-contaminated soils, the reproduction of nematodes, central actors in the soil food web, can be affected, with potentially negative implications for key soil functions, e.g., the regulation of soil biogeochemical cycles.

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Histopathological and molecular effects of microplastics in Eisenia andrei Bouché

Cited by 468 publications

References 31 publications

Microplastics as an emerging threat to terrestrial ecosystems

Microplastics as an emerging threat to terrestrial ecosystems

Prospects on the nano-plastic particles internalization and induction of cellular response in human keratinocytes

Microplastics Effects on Reproduction and Body Length of the Soil-Dwelling Nematode Caenorhabditis elegans

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