Histopathological analysis of Colossoma macropomum liver, treated with bioproducts used by controlling parasites

Abstract

Possible histological changes in the liver of Colossoma macropomum Cuvier, 1816 (tambaqui) generated by the use of products incorporated into the diet as a control method of Neoechinorhynchus buttnerae Golvan, 1956 were evaluated. 1036 juveniles tambaqui with 77±3.0g of average weight were used, maintained in 28 polyethylene tanks of 1000L, with constant aeration and water recirculation system, using UV filter. The experimental units (30 fish per experimental unit) were distributed into four treatments, with seven replicates. The products, incorporated in fish oil, were added to the feed, according to the treatments: control group with commercial feed (Nutrizon Karino 36% GW) (A); extract of garlic (B); organic acids (C) and extract of garlic + organic acids (D). A parasitic and histopathological evaluation was performed at time zero (T0). During the experiment, two samples were collected, with an interval of five weeks, evaluating 15 fish per experimental unit. At the end, 63 liver samples for histological analysis were collected. The evaluated animals presented the expected growth for the species. 87.2% of efficacy was obtained with the association extract of garlic + organic acids. Hepatic morphological lesions compatible with toxicological processes were not observed, indicating a low toxicity for tested concentrations.

Phytochemical prospecting and cytotoxicity assays of potential bioproducts to control parasites in Colossoma macropomum Cuvier

“…Specifically, for the case of fish, there are many risks of infections, since pathogens are characterized by their opportunism, constituting a gateway to secondary infections with high mortalities and loss of the entire squad [3]. This picture is also present in the state of Rondônia, where despite leading the ranking in the production of Amazonian fish (especially Tambaqui) and the third place when analyzing the production of farmed fish, according to the Anuário PEIXE BR 2021, studies related to health in fish farming are incipient, mainly diseases and parasites [4], both on causal agents, predisposing agents and pathological aspects, as well as the development of protocols that establish the experimental infestation of the main parasites that affect fish species.…”