Histone Deacetylase Inhibitor Significantly Improved the Cloning Efficiency of Porcine Somatic Cell Nuclear Transfer Embryos

Huang, Yongye; Tang, Xiaochun; Xie, Wanhua; Zhou, Yan; Dong, Liang; Yao, Chaogang; Zhou, Yang; Zhu, Junyan; Lai, Liangxue; Ouyang, Hongsheng; Pang, Daxin

doi:10.1089/cell.2011.0032

Cited by 40 publications

(34 citation statements)

References 43 publications

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“…Since then, many other HDACis, such as Scriptaid, valproic acid (VPA), or oxamflatin, have been applied to SCNT protocols to enhance reprogramming and increase cloning rates (Van Thuan et al, 2010;. VPA, for instance, is a short-chain fatty acid with HDACi activity that has proved to increase development and quality of mouse, bovine, and porcine SCNT embryos to a similar or even higher extent than TSA (Costa-Borges et al, 2010;Huang et al, 2011;Isaji et al, 2013;Kim et al, 2011;Miyoshi et al, 2010;Xu et al, 2012). Although the effects of each particular HDACi may vary among strains and species and some of the tested HDACis, such as aroyl pyrrolyl hydroxamide, have shown no beneficial effects (Van Thuan et al, 2009), it is clear from numerous reports that most HDACis improve nuclear reprogramming and cloning efficiency.…”

Section: Introductionmentioning

confidence: 99%

“…Given the results of more recent studies, higher VPA concentrations and/or longer treatment durations could further increase the beneficial effects of VPA (Huang et al, 2011;Isaji et al, 2013;Kim et al, 2011;Xu et al, 2012). Therefore, in the first part of the study, we sought to determine the optimal concentration and treatment duration for both PsA and VPA in terms of in vitro development of cloned mouse embryos and blastocyst quality.…”

Section: Introductionmentioning

confidence: 99%

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Psammaplin A Improves Development and Quality of Somatic Cell Nuclear Transfer Mouse Embryos

Mallol

Santaló

Ibáñez

2014

Cellular Reprogramming

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Faulty reprogramming of the donor somatic nucleus to a totipotent embryonic state by the recipient oocyte is a major obstacle for cloning success. Accordingly, treatment of cloned embryos with epigenetic modifiers, such as histone deacetylase inhibitors (HDACi), enhances cloning efficiency. The purpose of our study was to further explore the potential effect of valproic acid (VPA), used in previous studies, and to investigate the effect of psammaplin A (PsA), a novel HDACi, on the development and quality of cloned mouse embryos. To this aim, cloned embryos were treated with 5, 10, and 20 lM PsA or 2 and 4 mM VPA for 8-9 h (before and during activation) or 16 h or 24 h (during and after activation), and their in vitro developmental potential and blastocyst quality were evaluated. Treatments with 10 lM PsA and 2 mM VPA for 16 h were selected as the most optimal, showing higher blastocyst rates and quality. These treatments had no significant effects on the expression of Nanog, Oct4, and Cdx2 or on global histone and DNA methylation levels at the blastocyst stage, but both increased global levels of histone acetylation at early developmental stages. This was correlated with a two-fold (for VPA) and four-fold (for PsA) increase in full-term development, and a 11.5-fold increase when PsA was combined with the use of latrunculin A instead of cytochalasin B. In conclusion, PsA improves mouse cloning efficiency to a higher extent than VPA.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Psammaplin A Improves Development and Quality of Somatic Cell Nuclear Transfer Mouse Embryos

Mallol

Santaló

Ibáñez

2014

Cellular Reprogramming

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“…For example, when ESCs were synchronized to metaphase, a stage of cell cycle equivalent with metaphase II (MII) stage of matured oocyte, their cytoplast had the capacity to reprogram somatic cells (Du et al 2011). In addition, pretreatment of somatic cell with some small molecules, such as valproic acid (VPA), butyrate, and 5-aza-2′-deoxycytidine (AZA), could improve both the efficiency of SCNT and iPSCs Huangfu et al 2008;Mikkelsen et al 2008;Mali et al 2010;Huang et al 2011;Liu et al 2012). Furthermore, cell-free extracts from either oocytes or ESCs could be used to reprogram somatic cells (Taranger et al 2005;Miyamoto et al 2009).…”

Section: Matured Oocytes Were Endowed With Totipotent Developmental Pmentioning

confidence: 99%

Somatic cell reprogrammed by oocyte: process and barricade

Wang

et al. 2014

Animal Cells and Systems

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Somatic cell nuclear transfer (SCNT) is a technology in which a somatic nucleus is transferred into the cytoplasm of a matured oocyte -reconstructed embryos have the capacity to develop to term. Why somatic cells can be reprogrammed by oocytes -the answer for this question must exist in the cytoplasm of matured oocytes. In this review, totipotent characters of matured oocytes were discussed, which may confer matured oocytes with the capacity to reprogram somatic nucleus. Moreover, the procedure of SCNT also makes a possibility for somatic nucleus to be reprogrammed by oocytes. Compared with fertilized embryos, embryos derived from SCNT exhibit low developmental ability; the barricades in reprogramming process and their possible reasons were also discussed. This review maybe can benefit the mechanism research of SCNT technology and can make contribution for improving the efficiency of this technology.

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“…For example, Li et al (2008) observed a delay in SCNT pig morula compaction when embryos were treated with the HDACi trichostatin A (TSA). In contrast, Dai et al (2010) described that the onset of cavitation occurred earlier in SCNT mouse embryos treated with the HDACi m-carboxycinnamic acid bishydroxamide, and Huang et al (2011a) reported that SCNT pig embryos treated with the HDACi valproic acid (VPA) reached the blastocyst stage earlier than non-treated ones.…”

Section: Introductionmentioning

confidence: 99%

Morphokinetics of cloned mouse embryos treated with epigenetic drugs and blastocyst prediction

et al. 2016

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Time-lapse monitoring of somatic cell nuclear transfer (SCNT) embryos may help to predict developmental success and increase birth and embryonic stem cells (ESC) derivation rates. Here, the development of ICSI fertilized embryos and of SCNT embryos, non-treated or treated with either psammaplin A (PsA) or vitamin C (VitC), was monitored, and the ESC derivation rates from the resulting blastocysts were determined. Blastocyst rates were similar among PsA-treated and VitC-treated SCNT embryos and ICSI embryos, but lower for nontreated SCNT embryos. ESC derivation rates were higher in treated SCNT embryos than in non-treated or ICSI embryos. Time-lapse microscopy analysis showed that non-treated SCNT embryos had a delayed development from the second division until compaction, lower number of blastomeres at compaction and longer compaction and cavitation durations compared with ICSI ones. Treatment of SCNT embryos with PsA further increased this delay whereas treatment with VitC slightly reduced it, suggesting that both treatments act through different mechanisms, not necessarily related to their epigenetic effects. Despite these differences, the time of completion of the third division, alone or combined with the duration of compaction and/or the presence of fragmentation, had a strong predictive value for blastocyst formation in all groups. In contrast, we failed to predict ESC derivation success from embryo morphokinetics. Time-lapse technology allows the selection of SCNT embryos with higher developmental potential and could help to increase cloning outcomes. Nonetheless, further studies are needed to find reliable markers for full-term development and ESC derivation success.

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Histone Deacetylase Inhibitor Significantly Improved the Cloning Efficiency of Porcine Somatic Cell Nuclear Transfer Embryos

Cited by 40 publications

References 43 publications

Psammaplin A Improves Development and Quality of Somatic Cell Nuclear Transfer Mouse Embryos

Psammaplin A Improves Development and Quality of Somatic Cell Nuclear Transfer Mouse Embryos

Somatic cell reprogrammed by oocyte: process and barricade

Morphokinetics of cloned mouse embryos treated with epigenetic drugs and blastocyst prediction

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