Histological characteristics and markers of proliferation and differentiation in rat brain with experimental glioma

Ristić, Slavica; Mirić, Mirjana; Jović, Slađana; Ristić, Siniša; Karić, Jasmina

doi:10.2298/vsp121016044r

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…This was consistent with the previous findings, which demonstrated that C6 cells remain in an undifferentiated state and cannot be recognized by GFAP [43]. Based on their histological traits and biological behaviour, C6 glioma cells have been reported to correspond to human anaplastic astrocytoma of grade II/III [44], indicating that QDs-c(RGDyk)NP might be used as a tracer of human glioma in the future.…”

Section: He and Immunohistochemistry Staining Of Gliomasupporting

confidence: 92%

c(RGDyk)-modified nanoparticles encapsulating quantum dots as a stable fluorescence probe for imaging-guided surgical resection of glioma under the auxiliary UTMD

Xiong

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Surgical resection remains the preferred approach for some patients with glioblastoma (GBM), and eradication of the residual tumour niche after surgical resection is very helpful for prolonging patient survival. However, complete surgical resection of invasive GBM is difficult because of its ambiguous boundary. Herein, a novel targeting material, c(RGDyk)-poloxamer-188, was synthesized by modifying carboxyl-terminated poloxamer-188 with a glioma-targeting cyclopeptide, c(RGDyk). Quantum dots (QDs) as fluorescent probe were encapsulated into the self-assembled c(RGDyk)-poloxamer-188 polymer nanoparticles (NPs) to construct glioma-targeted QDs-c(RGDyk)NP for imaging-guided surgical resection of GBM. QDs-c(RGDyk)NP exhibited a moderate hydrodynamic diameter of 212.4 nm, a negative zeta potential of-10.1 mV and good stability. QDs-c(RGDyk)NP exhibited significantly lower toxicity against PC12 and C6 cells and HUVECs than free QDs. Moreover, in vitro cellular uptake experiments demonstrated that QDs-c(RGDyk)NP specifically targeted C6 cells, making them display strong fluorescence. Combined with ultrasound-targeted microbubble destruction (UTMD), QDs-c(RGDyk)NP specifically accumulated in glioma tissue in orthotropic tumour rats after intravenous administration, evidenced by ex vivo NIR fluorescence imaging of bulk brain and glioma tissue sections. Furthermore, fluorescence imaging with QDs-c(RGDyk)NP guided accurate surgical resection of glioma. Finally, the safety of QDs-c(RGDyk)NP was verified using pathological HE staining. In conclusion, QDs-c(RGDyk)NP may be a potential imaging probe for imaging-guided surgery.

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Section: He and Immunohistochemistry Staining Of Gliomasupporting

confidence: 92%

c(RGDyk)-modified nanoparticles encapsulating quantum dots as a stable fluorescence probe for imaging-guided surgical resection of glioma under the auxiliary UTMD

Xiong

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…Glioblastoma (GBM) is the most lethal malignant tumor arising in the brain for which no cure is currently available (Louis et al, ; Ristic, Miric, Jovic, Ristic, & Karic, ; Zong, Verhaak, & Canoll, ). The standard treatment for postoperative GBM patients is radiotherapy combined with chemotherapy (Stupp et al, ).…”

Section: Introductionmentioning

confidence: 99%

Astrocytes, the rising stars of the glioblastoma microenvironment

Brandao

Simon

Critchley

et al. 2018

Glia

124

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Glioblastoma (GBM) is an aggressive primary tumor, causing thousands of deaths worldwide every year. The mean survival of patients with GBM remains below 20 months despite current available therapies. GBM cells' interactions with their stromal counterparts are crucial for tumor development. Astrocytes are glial cells that comprise~50% of all brain cells and are therefore likely to establish direct contact with GBM cells. As other tumor cell types can hijack fibroblasts or immune cells to facilitate tumor growth, GBM cells can actually activate astrocytes, namely, the tumor associated astrocytes (TAAs), to promote GBM invasion in the healthy tissue. TAAs have thus been shown to be involved in GBM cells growth and limited response to radiation or chemotherapy (i.e., Temozolomide). Nevertheless, even though the interest in the cancer research community is increasing, the role of TAAs during GBM development is still overlooked.Yet, obtaining an in-depth understanding of the mechanisms by which TAAs influence GBM progression might lead to the development of new therapeutic strategies. This article therefore reports the different levels of GBM progression at which TAAs have been recently described to be involved in, including tumor cells' proliferation/invasion and resistance to therapies, especially through the activity of extracellular vesicles. K E Y W O R D S brain, glioblastoma, stromal cells, tumor associated astrocytes, tumor microenvironment

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Histological characteristics and markers of proliferation and differentiation in rat brain with experimental glioma

Abstract: Within the experimental conditions of the present research, C6 glioma did not demonstrate any relevant deviations concerning development, clinical symptomatology and macroscopic anatomy relative to those already described in the literature.

Cited by 2 publications

References 19 publications

c(RGDyk)-modified nanoparticles encapsulating quantum dots as a stable fluorescence probe for imaging-guided surgical resection of glioma under the auxiliary UTMD

c(RGDyk)-modified nanoparticles encapsulating quantum dots as a stable fluorescence probe for imaging-guided surgical resection of glioma under the auxiliary UTMD

Astrocytes, the rising stars of the glioblastoma microenvironment

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