Histological and immunohistochemical study of cardiac telocytes in a rat model of isoproterenol-induced myocardial infarction with a reference to the effect of grape seed extract

Nour, Mai Salah; Sarhan, Nahla Reda; Mazroa, Shireen A.; Gawish, Salwa A.

doi:10.1016/j.acthis.2017.09.007

Cited by 14 publications

(9 citation statements)

References 55 publications

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“…Similar findings were reported previously. 17-19 The wide spacing noticed in our study was in agreement with Patel et al, 14 who reported edema induced by capillary leakage that resulted from ROS-induced oxidative damage of ISO. Scalloping of sarcolemma, hypereosinophilia, and the contracted band could be attributed to the intracellular calcium overload leading to activation of protein kinases and impairment of mitochondrial phosphorylating capacity initiating stimulus for myocardial cell death and infarction.…”

Section: Discussionsupporting

confidence: 92%

Comparative Study of the Therapeutic Potential of Mesenchymal Stem Cells Derived from Adipose Tissue and Bone Marrow on Acute Myocardial Infarction Model

Omar¹,

Meleis²,

Arfa³

et al. 2019

Oman Med J

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ObjectivesStem cell therapy is a promising approach in the treatment of acute myocardial infarction (AMI). Mesenchymal stem cells (MSC) from bone marrow (BM-MSC) and adipose tissue (AT-MSC) are attractive and feasible for preclinical and clinical trials. In this study, we compared the therapeutic potential of BM-MSC and AT-MSC in repairing the hearts of rats with isoproterenol (ISO)-induced AMI.MethodsForty-two female rats were assigned into two groups; the optimization and the experimental group. The optimization groups were further subdivided into control group and the AMI induced group (using ISO). The experimental group was subdivided into AMI+cell-free media injected in the tail vein, AMI+BM-MSC, and AMI+AT-MSC groups treated with the intravenous injection of their respective cell types. Twenty-eight days after induction, electrocardiogram (ECG) was performed, and heart tissue samples were collected for histological assessment and cells tracing.ResultsMSC therapy repaired cardiac functions shown by the restoration of ST segment, QT and QRS intervals in the ECG when compared to the AMI group. Infarct area was significantly decreased, and cardiac tissue regeneration signs were shown on histopathological examination.ConclusionsBoth MSC sources proved to be equally efficient in the assessed parameters.

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Section: Discussionsupporting

confidence: 92%

Comparative Study of the Therapeutic Potential of Mesenchymal Stem Cells Derived from Adipose Tissue and Bone Marrow on Acute Myocardial Infarction Model

Omar¹,

Meleis²,

Arfa³

et al. 2019

Oman Med J

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“…Telocytes accompanying cardiomyocyte in primary culture: two-and three-dimensional culture environment [Zhou et al, 2010a] Cardiac telocytes were decreased during myocardial infarction and their therapeutic effects for ischaemic heart in rat [Zhao et al, 2013] Intramyocardial transplantation of cardiac telocytes decreases myocardial infarction and improves post-infarcted cardiac function in rats [Zhao et al, 2014] Comparative analysis of telomerase activity in CD117(+) CD34(+) cardiac telocytes with bone mesenchymal stem cells, cardiac fibroblasts and cardiomyocytes [Li et al, 2015] Histological and immunohistochemical study of cardiac telocytes in a rat model of isoproterenol-induced myocardial infarction with a reference to the effect of grape seed extract [Nour et al, 2017] J Cell Mol Med…”

Section: The Markers Used To Identify Cardiac Tcs Can Be Challengedmentioning

confidence: 99%

Myocardial Telocyte-Like Cells: A Review Including New Evidence

Iancu

Rusu

Mogoantă

et al. 2018

Cells Tissues Organs

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Telocytes (TCs) are a controversial cell type characterized by the presence of a particular kind of prolongations, known as telopodes, which are long, thin, and moniliform. A number of attempts has been made to establish the molecular phenotype of cardiac TCs (i.e., expression of c-kit, CD34, vimentin, PDGRFα, PDGRFβ, etc.). We designed an immunohistochemical study involving cardiac tissue samples obtained from 10 cadavers with the aim of determining whether there are TC-like interstitial cells that populate the interstitial space other than the mural microvascular cells. We applied the markers for CD31, CD34, PDGRFα, CD117/c-kit, and α-smooth muscle actin (α-SMA). We found that, in relation to two-dimensional cuts, the endothelial tubes could be misidentified as TC-like cells, the difference being the positive identification of endothelial lumina. Moreover, we found that cardiac pericytes express PDGRFα, CD117/c-kit, and α-SMA, and that they could also be misidentified as TCs when using light microscopy. We reviewed the respective values of the previously identified markers for achieving a clear-cut identification of cardiac TCs, highlighting the critical lack of specificity.

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“…Other reports have shown that proanthocyanidins are efficacious for treating human hypertension [ 220 ]. Consistent with these reports, experimental rodent models of cardiac disease demonstrated that GSP extract protected the heart in response to a high fat diet [ 217 , 218 , 221 ], doxorubicin-induced cardiotoxicity [ 222 , 223 , 224 , 225 , 226 ], heavy metal-induced cardiac stress [ 227 , 228 , 229 ], isoproterenol-induced HF [ 230 , 231 , 232 ] and I/R injury [ 233 , 234 , 235 , 236 , 237 ]. Additional studies reported that GSP extract lowered liver and blood cholesterol as well as triglycerides [ 238 , 239 , 240 , 241 ].…”

Section: Phytochemicalsmentioning

confidence: 59%

Food Bioactive HDAC Inhibitors in the Epigenetic Regulation of Heart Failure

Evans

Ferguson

2018

Nutrients

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Approximately 5.7 million U.S. adults have been diagnosed with heart failure (HF). More concerning is that one in nine U.S. deaths included HF as a contributing cause. Current HF drugs (e.g., β-blockers, ACEi) target intracellular signaling cascades downstream of cell surface receptors to prevent cardiac pump dysfunction. However, these drugs fail to target other redundant intracellular signaling pathways and, therefore, limit drug efficacy. As such, it has been postulated that compounds designed to target shared downstream mediators of these signaling pathways would be more efficacious for the treatment of HF. Histone deacetylation has been linked as a key pathogenetic element for the development of HF. Lysine residues undergo diverse and reversible post-translational modifications that include acetylation and have historically been studied as epigenetic modifiers of histone tails within chromatin that provide an important mechanism for regulating gene expression. Of recent, bioactive compounds within our diet have been linked to the regulation of gene expression, in part, through regulation of the epi-genome. It has been reported that food bioactives regulate histone acetylation via direct regulation of writer (histone acetyl transferases, HATs) and eraser (histone deacetylases, HDACs) proteins. Therefore, bioactive food compounds offer unique therapeutic strategies as epigenetic modifiers of heart failure. This review will highlight food bio-actives as modifiers of histone deacetylase activity in the heart.

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Histological and immunohistochemical study of cardiac telocytes in a rat model of isoproterenol-induced myocardial infarction with a reference to the effect of grape seed extract

Cited by 14 publications

References 55 publications

Comparative Study of the Therapeutic Potential of Mesenchymal Stem Cells Derived from Adipose Tissue and Bone Marrow on Acute Myocardial Infarction Model

Comparative Study of the Therapeutic Potential of Mesenchymal Stem Cells Derived from Adipose Tissue and Bone Marrow on Acute Myocardial Infarction Model

Myocardial Telocyte-Like Cells: A Review Including New Evidence

Food Bioactive HDAC Inhibitors in the Epigenetic Regulation of Heart Failure

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