Histological and Immunohistochemical Implications of Ketamine Protracted Doses as a Novel Candidate in Antidepressant Treatmenton Adult Rat Cerebellar Cortex.

Asker, SamarA.; Mazroa, ShireenA.; Sabri, Youmna

doi:10.21474/ijar01/613

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present work, Immunohistochemical study of calretinin proteins revealed that Purkinje cells of group II showing a highly significant increase in calretinin immunoreaction as compared to group I. However, in group III they showed a highly significant decrease in the reaction compared to group II and these results were reported by previous studies [45] . In calretinin knockout mice, the cerebellum resulted in unexpected positive calretinin immunoreactivity in Purkinje cells as marked abnormalities in the Purkinje cell firing with modifications of both simple and complex spike [46] .…”

Section: Discussionsupporting

confidence: 85%

Histological and immunohistochemical study on the effect of zinc oxide nanoparticles on cerebellar cortex of adult male albino rats

Abdel-aziz

Mekawy

Ibrahem

2019

Egyptian Journal of Histology

View full text Add to dashboard Cite

Background: Zinc oxide nanoparticles (ZnONPs) are one of metal nanoparticles that have widespread use in many fields. Objective: To investigate the effect of ZnONPs on cerebellar cortex of rats through histological and immunohistochemical study. Materials and methods: Thirty adult male albino rats were divided into three groups; group I (control), Group II (ZnONP-1 treated group) which received orally 50 mg/kg of ZnONP for two months and Group III (ZnONP-1I treated group) which received orally 200 mg/kg of ZnONP for two months. Specimens of the cerebellar cortex were processed for histological and immunohistochemical study. Morphometric and statistical analysis were carried out. Results: Group II showed Purkinje cells were crowded in many layers surrounded by perineuronal vacuoles and had pyknotic nuclei. They had cytoplasmic vacuoles and perinuclear Golgi apparatus revealed fragmented dilated cisternae. Nearby Bergmann astrocyte cells had highly vacuolated cytoplasm and the nerve fibers were also affected and showed dysmyleination (disrupted myelin sheath). Immunohistochemical study of the same group showed Purkinje cell cytoplasm had positive immunoreactions for calretinin proteins. In group III, there was a wide spread of neuronal affection to the degree of loss of many of Purkinje cells leaving empty spaces. Ultrastructurly, their cytoplasm appeared with multiple variable sized and had dilated mitochondria with disrupted cristae. The Bergmann astrocytes revealed nuclei with disrupted nuclear envelope and nearly absence their cytoplasmic organelle and there was more affection to the nerve fibers in the form of vacuolated axoplasm and demyelination (areas of myelin loss) beside dysmyleination. Immunohistochemical study of group III showed Purkinje cells cytoplasm with negative immunoreactions for calretinin proteins. Conclusion: Intake of ZnONPs induced various adverse alterations in the histological and immunohistochemical structures of cerebellar cortex indicating the occurrence of neurotoxicity. These changes were exaggerated with increasing the dose of their intake.

show abstract

Section: Discussionsupporting

confidence: 85%

Histological and immunohistochemical study on the effect of zinc oxide nanoparticles on cerebellar cortex of adult male albino rats

Abdel-aziz

Mekawy

Ibrahem

2019

Egyptian Journal of Histology

View full text Add to dashboard Cite

show abstract

“…As regard calretinin immunoreaction, negative reaction was detected in granule cells of the ischemic group as compared to the control group. Uniform findings were also found by Asker et al [15] . Decrease calretinin expression in granule cells as a result of calcium channel dysfunction was found to cause abnormal neuronal excitability [57] , impairment in the motor control in mouse cerebellum [58,59] .…”

Section: Discussionsupporting

confidence: 80%

“…It is demonstrate that its on hippocampal are mediated through ERβ [14] . Calretinin is a calcium-bindig protein which was shown to be present in many brain regions [15] . Regulation of calcium homeostasis may be a key in the protection of the brain from injury [16] .…”

Section: Introductionmentioning

confidence: 99%

Efficacy of cerebrolysin on dentate gyrus of hippocampus after experimentally induced acute ischemic stroke in adult albino rats (Histological, immunohistochemical and biochemical study)

Abdel-aziz

Ahmed

Mohammed

et al. 2019

Egyptian Journal of Histology

View full text Add to dashboard Cite

Background: Acute ischemic stroke (AIS) is a brain medical disorder characterized by the sudden loss of blood circulation to an area of the brain, resulting in a loss of its neurologic function. Cerebrolysin is a mixture of neuropeptides and free amino acids. Aim of the work:This study aimed to evaluate the role of cerebrolysin in ameliorating the histological,immunohistochemic al and biochemical harm in post ischemic stroke and also to assess its dose dependent effect. Materials and Methods: Thirty adult male rats were divided into 3 equal groups; control, ischemic and post ischemic treated groups. After dissecting hippocampal dentate gyrus prepared sections were stained with hematoxylin and eosin, Erβ and calretinin proteins. Oxidative stress parameters, TNF α, and HSP-70 assay. Also, Gh receptor gene expression and DNA fragmentation test were measured. Immunoperoxidase reactions for GFAPin astrocyte was estimated. Statistical analysis was conducted. Results: Ischemia group showed decrease number of granule cells with small dark stained nuclei, areas of cell loss and numerous spindle shaped cells in the sub-granular zone. A faint positive immunoreaction for ERβ in nuclei of granular cells and negative reaction for calretinin was detected in granule cells. Antioxidant enzymes in the brain tissue as MDA,TNF α and HSP-70 were significantly elevated in ischemic group compared to control and post ischemic treated groups. Gh receptor gene were decreased, while Fragmentation index of DNA was significantly increased. Administration of 2.5 mg/ kg cerebrolysinshowed partial improvement, whereas, 5 mg/kg dose displayed more ameliorative effects. Increased GFAP immune-expression in the cytoplasm of astrocytes in ischemic group compared to control and post ischemic treated groups Conclusion: Cerebrolysin was effective in experimentally induced AIS (Acute ischemic stroke) in a dose-dependent manner as proved by improving the histological structure, immunohistochemical reactions and biochemical parameters of the dentate gyrus of hippocampus in adult male albino rats.

show abstract

Histological and Immunohistochemical Implications of Ketamine Protracted Doses as a Novel Candidate in Antidepressant Treatmenton Adult Rat Cerebellar Cortex.

Cited by 2 publications

References 0 publications

Histological and immunohistochemical study on the effect of zinc oxide nanoparticles on cerebellar cortex of adult male albino rats

Histological and immunohistochemical study on the effect of zinc oxide nanoparticles on cerebellar cortex of adult male albino rats

Efficacy of cerebrolysin on dentate gyrus of hippocampus after experimentally induced acute ischemic stroke in adult albino rats (Histological, immunohistochemical and biochemical study)

Contact Info

Product

Resources

About