2014
DOI: 10.1590/s0100-736x2014001300012
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Abstract: Resumo: Este estudo teve como objetivo avaliar as possíveis alterações histopatológicas hepática de tilápias do Nilo alimentadas com dietas contendo silagem biológica de pescado com diferentes concentrações protéicas. Foram utilizados 180 juvenis alimentados com dietas contendo três níveis de proteína (20, 24 and 28% PB), e duas proporções de silagem biológica (¼ e ½) durante 75 dias. Os fragementos de fígado foram fixados em Bouin e inclusos em Histosec®. Posteriorente foram cortados em microtomo com espessur… Show more

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Cited by 6 publications
(13 citation statements)
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References 9 publications
(13 reference statements)
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“…Many feed additives have shown beneficial results in terms of intestinal morphology of fish such as tilapia (Honorato et al, 2014;Schwarz et al, 2011) and lambari Astyanax bimaculatus (Lima, 2014).…”
Section: Intestinal Analysis -Measurement Of Villi and Goblet Cell mentioning
confidence: 99%
“…O fígado é fundamental para o metabolismo dos nutrientes e nos dá respostas do estado nutricional em peixes, essas alterações podem ser resultado de uma dieta desbalanceada, exposição a substâncias químicas ou drogas, respondendo com adaptações, lesão ou morte celular (Bolla et al, 2011;Raskovic et al, 2011;Honorato et al, 2014). Neste estudo realizado, não foi observado comprometimento das células hepáticas mesmo apresentando aumento na estrutura dos hepatócitos em um dos grupos tratados.…”
Section: Resultsunclassified
“…The liver is the organ responsible for some essential functions in the organism, and alterations in this organ may indicate damage related to metabolism, biotransformation, and excretion of certain substances [27,28]. Once liver damage is established, the homeostasis of the organism as a whole can be affected by interfering with some of the organ's basic functions such as the synthesis and biotransformation of molecules [29].…”
Section: Discussionmentioning
confidence: 99%
“…All fish underwent surgery to remove fragments of the liver, which were fixed in formalin (10% formalin +4% tetraborate) for 24 h and then into alcohol 70% until histological analysis. Subsequently, all fragments were dehydrated, diaphanized, and then embedded in paraffin to perform histological cuts at 5 µm, posteriorly stained with haematoxylin‐eosin (HE) (Behmer & Tolosa, 1976; Honorato et al, 2014). The severity degree of infection in the liver was determined using the symbol (+) according Van Dyk et al (2007).…”
Section: Methodsmentioning
confidence: 99%
“…Subsequently, all fragments were dehydrated, diaphanized, and then embedded in paraffin to perform histological cuts at 5 µm, posteriorly stained with haematoxylin-eosin (HE) (Behmer & Tolosa, 1976;Honorato et al, 2014). The severity degree of infection in the liver was determined using the symbol (+) according Van Dyk et al (2007).…”
Section: Histological Analysismentioning
confidence: 99%