Histochemistry of Trichomes

Peterson, R. L.; Vermeer, Janet

doi:10.1007/978-1-4899-5355-1_4

Cited by 21 publications

(22 citation statements)

References 48 publications

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“…A recently developed procedure (Gershenzon et al, 1992) for the isolation of secretory cells from the peltate glandular trichomes of peppermint has been shown to yield clusters of intact cells that maintain a high degree of metabolic function. A common feature of many glandular trichomes, including those of peppermint, is the presence of a heavily cutinized, lipid-impregnated cell wall surrounding the stalk cell (Amelunxen 1965;Peterson and Vermeer 1984). Under appropriate conditions, mechanized abrasion of the leaf surface with glass beads causes fragmentation of both this modified cell wall and the cuticle covering the subcuticular space, releasing the secretory cells as an intact disc.…”

Section: Discussionmentioning

confidence: 99%

Morphology and monoterpene biosynthetic capabilities of secretory cell clusters isolated from glandular trichomes of peppermint (Mentha piperita L.)

McCaskill

Gershenzon

Croteau

1992

Planta

157

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Secretory cells were isolated from the monoterpene-producing glandular trichomes (peltate form) of peppermint as clusters of eight cells each. These isolated structures were shown to be non-specifically permeable to low-molecular-weight, water-soluble cofactors and substrates. Short incubation periods with the polar dye Lucifer yellow iodoacetamide (Mr=660) resulted in a uniform staining of the cytoplasm, with exclusion of the dye from the vacuole. The molecular-weight exclusion limit for this permeability was shown to be less than approx. 1800, based on exclusion of fluorescein-conjugated dextran (Mr ∼ 1800). Intact secretory cell clusters very efficiently incorporated [(3)H]geranyl pyrophosphate into monoterpenes. The addition of exogenous cofactors and redox substrates affected the distribution of monoterpenes synthesized from [(3)H]geranyl pyrophosphate, demonstrating that the cell clusters were permeable to these compounds and that the levels of endogenous cofactors and redox substrates were depleted in the isolated cells. When provided with the appropriate cofactors, such as NADPH, NAD(+), ATP, ADP and coenzyme A, the isolated secretory cell clusters incorporated [(14)C]sucrose into monoterpenes, indicating that these structures are capable of the de-novo biosynthesis of monoterpenes from a primary carbon source, and that they maintain a high degree of metabolic competence in spite of their permeable nature.

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Section: Discussionmentioning

confidence: 99%

Morphology and monoterpene biosynthetic capabilities of secretory cell clusters isolated from glandular trichomes of peppermint (Mentha piperita L.)

McCaskill

Gershenzon

Croteau

1992

Planta

157

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“…Some of the epidermal cells grow out to a considerable length, giving rise to trichomes. Trichomes are often classified as being glandular or non-glandular, based on whether or not they function as secretory structures (Peterson & Vermeer, 1984). The unique characteristics of trichomes have traditionally been used as a taxonomic key in plant classification (Wagner, 1991;Xiang et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Structural Features of Glandular and Non-glandular Trichomes in Three Species of Mentha

Choi¹,

Kim²

2013

Applied Microscopy

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The trichomes on leaves of three species of Mentha such as M. spicata, M. suaveolens, and M. piperita var were examined by scanning electron microscopy. Simple non-glandular trichomes and peltate glandular trichomes were distinctively occurred on these leaves. In M. spicata, short and sharp non-glandular trichomes were dominantly appeared on adaxial surface, whereas capitate glandular trichomes were commonly localized on abaxial surface. In M. suaveolens, non-glandular trichomes were identified with simple unbranched and branched. Unbranched trichomes which were sharp and pointed in shape occurred on adaxial surface, however, branched and v-shaped trichomes appeared abundantly on abaxial surface. Peltate trichomes consisted of a large eight-celled head. Small capitates trichomes consisted of a cylindrical head with an one-celled uniseriate stalk. In M. piperita var, single non-glandular trichomes and peltate trichomes were present on adaxial surface. Small capitate trichomes consisted of a globose unicellular head with a two-or three-celled uniseriate stalk. Peltate trichomes were distinctly present on abaxial surface, whereas they were not observed on adaxial surface. The trichomes were less dense in this species. Peltate trichomes consisted of a large eight-celled head, with an enlarged secretory cavity, attached to an one-celled short stalk.

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“…14) shows specific Bismarck brown uptake in one part of the trichome structure while other parts and subjacent tissues were dominated by alcian blue. Glandular trichomes are well known in Pelargoniurn, and the type structures (Peterson and Vermeer 1984) are short and squat. These did not show affinity for Bismarck brown in our triple stain and appeared to be entirely different structures than those shown in Fig. 14 (data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…Previous investigations of trichomes have required nonpolar stains, light microscopy other than histological bright field (e. g., histochemical fluorescence, dark field and polarized light) and electron microscopy (Peterson and Vermeer 1984). Our triple stain combined with His-toChoiceB fixation and greatly abbreviated slide preparation should bring certain aspects of trichome investigation within reach of laboratories equipped only with a rotary microtome and bright field microscopy.…”

Section: B Iotechn Ic and H Istochem Istrymentioning

confidence: 98%

Staining Paraffin Extracted, Alcohol Rinsed and Air Dried Plant Tissue with an Aqueous Mixture of Three Dyes

1998

Biotechnic & Histochemistry

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A staining solution containing alcian blue 8GX, Bismarck brown Y and safranin O was prepared with 0.1 M sodium acetate buffer, pH 5.0. Paraffin was extracted with MicroClear solvent from 10 microm tissue sections mounted on slides. Paraffin solvent was removed by rinsing with isopropanol, and tissues were air dried. Slides with bare dry tissue sections were immersed in the triple stain and structures could be distinguished within 30 min as follows: nonlignified cell walls, blue; lignified cell walls, nuclei and chloroplasts, red; and cuticle, brown or yellow-brown. Excess staining solution was removed by rinsing with tap water, and the tissues were air dried again. Coverslips were affixed with resin over the stained dry tissues. This novel procedure was tested with immature tomato fruit, mature apple fruit, and various leaf and stem specimens of dogwood, laurel, pawpaw, poinsettia and zonal geranium.

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Histochemistry of Trichomes

Cited by 21 publications

References 48 publications

Morphology and monoterpene biosynthetic capabilities of secretory cell clusters isolated from glandular trichomes of peppermint (Mentha piperita L.)

Morphology and monoterpene biosynthetic capabilities of secretory cell clusters isolated from glandular trichomes of peppermint (Mentha piperita L.)

Structural Features of Glandular and Non-glandular Trichomes in Three Species of Mentha

Staining Paraffin Extracted, Alcohol Rinsed and Air Dried Plant Tissue with an Aqueous Mixture of Three Dyes

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