Histochemistry and Cell Wall Specialization of Oil Cells related to the Essential Oil Accumulation in the Bark of<i>Cinnamomum cassia</i>Presl. (Lauraceae)

Geng, Shilei; Cui, Zhao-Xue; Shu, Bin; Zhao, Shuai; Yu, Xiaojun

doi:10.1626/pps.15.1

Cited by 21 publications

(15 citation statements)

References 37 publications

(54 reference statements)

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“…Essential oils are concentrated liquids containing volatile secondary metabolites. The oil cells are commonly present in roots, stems, bark, fruits and leaves in Lauraceae trees [41]–[45], and are also observed in all tissues analyzed in Persea americana [46]. This distribution of oil cells, with probable tissue-specific variability in abundance and composition, could explain the correlations found in our study among several mono- and sesquiterpenes all along the proximal-distal axis.…”

Section: Discussionsupporting

confidence: 64%

“…Several studies have investigated the ultrastructure, composition and distribution of oil cells in the Lauraceae family, including avocado trees, using light, fluorescence and electron microscopy. In this plant family, oil cells are the primary site for essential oil biosynthesis, secretion and storage [41]. Essential oils are concentrated liquids containing volatile secondary metabolites.…”

Section: Discussionmentioning

confidence: 99%

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Terpenoid Variations within and among Half-Sibling Avocado Trees, Persea americana Mill. (Lauraceae)

Niogret¹,

Epsky²,

Schnell³

et al. 2013

PLoS ONE

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Chemical analyses were conducted to determine the qualitative and quantitative differences in monoterpenes and sesquiterpenes in plant material from avocado trees, Persea americana Mill. (Lauraceae). The initial study analyzed plant material sampled from the trunk to the leaves through different branch diameters to quantify proximo-distal spatial differences within a tree. All trees were seedlings initiated from a single maternal tree. Two-way analysis of variance was conducted on 34 chemicals that comprised at least 3% of the total chemical content of at least one tree and/or location within a tree. There were significant interactions between genotype and location sampled for most chemicals. Parentage analysis using microsatellite molecular markers (SSR's) determined that the four trees had three fathers and that they represented two full-siblings and two half-sibling trees. Descriptive discriminant analysis found that both genotype and location within a tree could be separated based on chemical content, and that the chemical content from full-siblings tended to be more similar than chemical content from half-siblings. To further explore the relationship between genetic background and chemical content, samples were analyzed from leaf material from 20 trees that included two sets of full-sibling seedling trees, the maternal tree and the surviving paternal tree. Descriptive discriminant analysis found good separation between the two full-sibling groups, and that the separation was associated with chemistry of the parental trees. Six groups of chemicals were identified that explained the variation among the trees. We discuss the results in relation to the discrimination process used by wood-boring insects for site-selection on host trees, for tree selection among potential host trees, and the potential use of terpenoid chemical content in chemotaxonomy of avocado trees.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Terpenoid Variations within and among Half-Sibling Avocado Trees, Persea americana Mill. (Lauraceae)

Niogret¹,

Epsky²,

Schnell³

et al. 2013

PLoS ONE

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“…The oil cells were spherical or rarely oval in shape and sheathed by a layer of cells with suberized and lignified walls. Previous studies [12] reported the presence of lignin and suberin in the walls of the oil cells in Cinnamomum cassia. In the present study, the oil cell walls were stained pinkish red in phloroglucinol/HCl [13] and emitted an intense blue-white autofluorescence under UV light excitation at 330-380 nm (▶ Fig.…”

Section: Resultsmentioning

confidence: 91%

Which Bay Leaf is in Your Spice Rack? – A Quality Control Study

2017

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The accurate identification of bay leaf in natural products commerce may often be confusing as the name is applied to several different species of aromatic plants. The true "bay leaf", also known as "bay laurel" or "sweet bay", is sourced from the tree , a native of the Mediterranean region. Nevertheless, the leaves of several other species including, and are commonly substituted or mistaken for true bay leaves due to their similarity in the leaf morphology, aroma, and flavor. Substitute species are, however, often sold as "bay leaves". As such, the name "bay leaf" in literature and herbal commerce may refer to any of these botanicals. The odor and flavor of these leaves are, however, not the same as the true bay leaf, and for that reason they should not be used in cooking as a substitute for. Some of the bay leaf substitutes can also cause potential health problems. Therefore, the correct identification of the true bay leaf is important. The present work provides a detailed comparative study of the leaf morphological and anatomical features of and its common surrogates to allow for correct identification.

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“…As a result, their evaporation is delayed, and their cytotoxicity is contained, until they are needed for the defence of the plant against insects or microbes. Well‐studied examples of such tissues include glandular trichomes, idioblasts and resin ducts (Geng et al ., ; Lange, ; Mewalal et al ., ; Tissier et al ., ). All these plant organs provide a core of hydrophobic material where terpenes can accumulate, which is isolated from the surrounding tissue by a protective layer.…”

Section: Introductionmentioning

confidence: 99%

Engineering storage capacity for volatile sesquiterpenes in Nicotiana benthamiana leaves

Delatte

Scaiola

Molenaar

et al. 2018

Plant Biotechnology Journal

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SummaryPlants store volatile compounds in specialized organs. The properties of these storage organs prevent precarious evaporation and protect neighbouring tissues from cytotoxicity. Metabolic engineering of plants is often carried out in tissues such as leaf mesophyll cells, which are abundant and easily accessible by engineering tools. However, these tissues are not suitable for the storage of volatile and hydrophobic compound such as sesquiterpenes and engineered volatiles are often lost into the headspace. In this study, we show that the seeds of Arabidopsis thaliana, which naturally contain lipid bodies, accumulate sesquiterpenes upon engineered expression. Subsequently, storage of volatile sesquiterpenes was achieved in Nicotiana benthamiana leaf tissue, by introducing oleosin‐coated lipid bodies through metabolic engineering. Hereto, different combinations of genes encoding diacylglycerol acyltransferases (DGATs), transcription factors (WRINKL1) and oleosins (OLE1), from the oil seed‐producing species castor bean (Ricinus communis) and Arabidopsis, were assessed for their suitability to promote lipid body formation. Co‐expression of α‐bisabolol synthase with Arabidopsis DGAT1 and WRINKL1 and OLE1 from castor bean promoted storage of α‐bisabolol in N. benthamiana mesophyll tissue more than 17‐fold. A clear correlation was found between neutral lipids and storage of sesquiterpenes, using synthases for α‐bisabolol, (E)‐β‐caryophyllene and α‐barbatene. The co‐localization of neutral lipids and α‐bisabolol was shown using microscopy. This work demonstrates that lipid bodies can be used as intracellular storage compartment for hydrophobic sesquiterpenes, also in the vegetative parts of plants, creating the possibility to improve yields of metabolic engineering strategies in plants.

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Histochemistry and Cell Wall Specialization of Oil Cells related to the Essential Oil Accumulation in the Bark ofCinnamomum cassiaPresl. (Lauraceae)

Cited by 21 publications

References 37 publications

Terpenoid Variations within and among Half-Sibling Avocado Trees, Persea americana Mill. (Lauraceae)

Terpenoid Variations within and among Half-Sibling Avocado Trees, Persea americana Mill. (Lauraceae)

Which Bay Leaf is in Your Spice Rack? – A Quality Control Study

Engineering storage capacity for volatile sesquiterpenes in Nicotiana benthamiana leaves

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