Histochemical study on the Paneth cell in the rat.

Riecken, E. O.; Pearse, A. G. E.

doi:10.1136/gut.7.1.86

Cited by 45 publications

(14 citation statements)

References 35 publications

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“…Hally [14] could find no evidence that the electron dense lysosomes are either discharged into the crypt lumen or transformed directly into secretory granules. Riecken and Pearse [31] showed that enzyme activity in the Paneth cell was at its weakest in the region of the secretory granules, and strongest in the region of the Golgi apparatus where the electron dense bodies are found. This observa tion receives confirmation from Lewin [21] who found some aminopeptidase in the Paneth cell, but not localised to the secretory granules.…”

Section: Discussionmentioning

confidence: 99%

Paneth Cell Secretion

Gent¹,

Creamer²

1972

Digestion

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The secretion of Paneth cells in the rat has been stimulated by pilocarpine, the small intestinal secretions have been collected and their free amino acid concentrations measured. Comparison with a control group of animals not given pilocarpine showed a statistically significant increase in the free amino acid concentrations. ⁶⁵Zn has been used to label Paneth cells in experiments which indicate that this increase in luminal amino acid concentrations is the result of the action of pilocarpine on the Paneth cell. Experiments using tritiated thymidine indicate that pilocarpine does not influence the rate of cell turnover in the rat small intestine. We conclude that the Paneth cell secretion is composed of amino acids.

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Section: Discussionmentioning

confidence: 99%

Paneth Cell Secretion

Gent¹,

Creamer²

1972

Digestion

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“…No C c. yacare não observamos as primeiras, mas enormes glândulas ovais, lobuladas entre o conjuntivo subepitelial e a camada muscular. Vários autores descreve· ram glândulas lobulares, holócrinas em crocodilianos (RIEKEN & PEARSE, 1966;VOELTSKOW, 1902;DISSELHORST, 1904;TAGUCHl, 1920;BAKER, 1942). Parece que essas glândulas estão relacionadas com a maturidade e estimulação sexual.…”

Section: Discussão E Conclusõesunclassified

Histologia geral dos intestinos do Caiman crocodilus yacare (Daudin, 1802) (Crocodilia: Reptilia)

et al. 1990

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“…RIEcKEN and PEARSE (1966) suggested that the Paneth cell secretory granules are lysosomal in function, because of their positive cytochemical reactivities for lysosomal enzymes such as acid phosphatase and esterases. It has been well established that one of the main substances contained in the Paneth cell secretory granules is lysozyme (see review by SANDOW and WHITEHEAD, 1979;HEITZ and WEGMANN, 1980; LOPEZ-LEWELLYN and ERLANDSEN, 1980;RODNING et al, 1982).…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure of paneth cells in germ-free rats, with special reference to the secretory granules and lysosomes.

Satoh

1984

Arch. Histol. Jap., Arch. Histol. Jpn

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Summary.Ultrastructure and acid phosphatase activity in Paneth cells, which are thought to play a role in regulation of the intestinal flora, were observed in control and germ-free rats (5 weeks of age, male). Examination of the germ-free rats revealed: 1) secretory granules with coarse and a low-electron dense matrix in the duodenal, jejunal and ileal Paneth cells; 2) numerous and larger lysosomes in ileal Paneth cells ; and 3) contact or fusion between the secretory granules and lysosomes in ileal Paneth cells. Acid phosphatase activities in Paneth cells were observed in the GERL of NOVIKOFF (1964) and lysosomes in germ-free as well as control rats.None of the secretory granules showed any acid phosphatase activity. The fact that the lysosomes fused with the secretory granules suggests that the process of crinophagy occurred in the Paneth cells of germ-free rats.The function of Paneth cells has remained obscure, even though their study was initiated over a century ago (ScHWALBE,1872). RIEcKEN and PEARSE (1966) suggested that the Paneth cell secretory granules are lysosomal in function, because of their positive cytochemical reactivities for lysosomal enzymes such as acid phosphatase and esterases. It has been well established that one of the main substances contained in the Paneth cell secretory granules is lysozyme (see review by SANDOW and WHITEHEAD, 1979;HEITZ and WEGMANN, 1980; LOPEZ-LEWELLYN and ERLANDSEN, 1980;RODNING et al., 1982). As lysozyme breaks down the mucopeptidic cell walls of bacteria, it follows that Paneth cells may play a role in regulation of the intestinal flora. ERLANDSEN and CHASE (1972a, b) suggested that Paneth cells are "fixed phagocytes" in the intestinal crypt.For elucidation of the function of Paneth cells, examination of their ultrastructural characteristics in germ-free animals is in order. OTTO and LEWERENZ (1973) observed these cells in germ-free rats containing many degenerative secretory granules, and suggested that there might be no stimulation for the occurrence of exocytosis in the Paneth cells in germ-free state.This study aims to reveal the differences in the ultrastructure of duodenal, jejunal and ileal Paneth cells between control and germ-free rats, with special reference to the secretory granules and lysosomes. The ultrastructural localization of acid phosphatase activity in the cells in germ-free rats will also be described.

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Histochemical study on the Paneth cell in the rat.

Cited by 45 publications

References 35 publications

Paneth Cell Secretion

Paneth Cell Secretion

Histologia geral dos intestinos do Caiman crocodilus yacare (Daudin, 1802) (Crocodilia: Reptilia)

Ultrastructure of paneth cells in germ-free rats, with special reference to the secretory granules and lysosomes.

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