Histamine release from isolated rat mast cells by neurotensin and other peptides

Abstract

The histamine-releasing activity of some basic peptides was tested on mast cells from Sprague-Dawley or Brown % 70. Norway rats. Somatostatin, bombesin, vasoaetive intestinal H~ peptide (VIP) or substance P did not give any histamine release release in concentrations up to 10 -6 M. Somatostatin in 10 -2 M concentration released 25% of total histamine. However, 50. neurotensin induced a significant histamine release from Brown Norway mast ceils at 10 -8 M. The neurotensininduced histamine release was found to be selective and in some aspects similar to that induced by compound 48/80.

Plasma protein extravasation induced by mammalian tachykinins in rat skin: influence of anaesthetic agents and an acetylcholine antagonist

“…However, this analogue was found to increase the plasma protein extravasation, possibly by promoting the release of histamine and acetylcholine. Indeed, SP analogues containing Dtryptophan are potent at inducing histamine release from rat mast cells Fewtrell et al, 1982;Sydbom, 1982;Devillier et al, 1985) and are also more potent than SP in producing wheal and flare responses in human skin . In addition, these SP analogues possess considerable inherent stimulatory activity when tested for both [3H]-acetylcholine release and myotropic activity in the guinea-pig ileum (Hawcock et al, 1982;Featherstone et al, 1986).…”

A substance P antagonist inhibits vagally induced increase in vascular permeability and bronchial smooth muscle contraction in the guinea pig

“…via ajugular vein catheter. Because of the histamine-releasing properties of the SP antagonist (18), the animals were first treated with mepyramine at 1 mg/kg and cimetidine at 1 mg/kg. These histamine antagonists did not influence the insufflation pressure or permeability response to SP, capsaicin, or vagus nerve stimulation.…”

Biological evaluation of substance P antagonists

“…However, the first antagonists described had a low potency which limited their usefulness as pharmacological tools. Further, they possessed histamine-releasing properties (Fewtrell et al, 1982;Hakanson et al, 1982;Sydbom, 1982) which complicated the analysis of their mode of action. Subsequently other SP antagonists have been synthesized in order to eliminate these shortcomings.…”