Highly specific, sensitive and rapid enzyme immunoassays for the measurement of acetaminophen in serum

Abuknesha, Ramadan A.; Paleodimos, Michael; Jeganathan, Fiona

doi:10.1007/s00216-011-5272-0

Cited by 5 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, it is possible that the novel mechanism of acetaminophen shown here is implicated in the high risk of the development of bone fracture and fragile bone observed in the population prescribed with the widely used analgesics (Warner et al 1999;Williams et al 2011;Wheatley et al 2019). The clinically critical acetaminophen concentration of serum was considered to be ranged between 50 to 250 μg/mL (approximately 0.3 to 1.6 μM (Abuknesha et al 2011). It has also been reported that high-dose rectal and oral acetaminophen administration (4,000 mg in total) caused 141.2 ± 52.1 μM in its maximum serum concentration (Hahn et al 2000).…”

Section: Effects Of Sp600125 On the Expression Levels Of Osteoprotege...mentioning

confidence: 99%

Acetaminophen reduces osteoprotegerin synthesis stimulated by PGE2 and PGF2α in osteoblasts: attenuation of SAPK/JNK but not p38 MAPK or p44/p42 MAPK

et al. 2021

View full text Add to dashboard Cite

Acetaminophen is one of the most widely used analgesic and antipyretic medicines, whose long-period use has reportedly been associated with an increased risk of bone fracture. However, the mechanism underlying this undesired effect remains to be investigated. The homeostatic control of bone tissue depends on the interaction between osteoblasts and osteoclasts. Osteoprotegerin produced by osteoblasts is known to play an essential role in suppressing osteoclast induction. We have previously reported that prostaglandin (PG) E 2 and PGF 2α induce osteoprotegerin synthesis through p38 mitogen-activated protein kinase (MAPK), p44/p42 MAPK and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) in osteoblast-like MC3T3-E1 cells. In the present study, we investigated the effects of acetaminophen on the osteoprotegerin synthesis induced by PGE 2 and PGF 2α in MC3T3-E1 cells. Acetaminophen significantly suppressed the osteoprotegerin release stimulated by PGE 2 and PGF 2α . The PGE 2 -induced expression of osteoprotegerin mRNA was also reduced by acetaminophen. Acetaminophen markedly downregulated the phosphorylation of SAPK/JNK stimulated by PGE 2 and PGF 2α , but not those of p38 MAPK or p44/p42 MAPK. SP600125, an inhibitor of SAPK/JNK, suppressed the levels of PGE 2 -and PGF 2α -upregulated osteoprotegerin mRNA expression. Taken together, these results strongly suggest that acetaminophen reduces the PGE 2 -and PGF 2α -stimulated synthesis of osteoprotegerin in osteoblasts, and that the suppressive effect is exerted via attenuation of SAPK/JNK. These findings provide a molecular basis for the possible effect of acetaminophen on bone tissue metabolism.

show abstract

Section: Effects Of Sp600125 On the Expression Levels Of Osteoprotege...mentioning

confidence: 99%

Acetaminophen reduces osteoprotegerin synthesis stimulated by PGE2 and PGF2α in osteoblasts: attenuation of SAPK/JNK but not p38 MAPK or p44/p42 MAPK

et al. 2021

View full text Add to dashboard Cite

show abstract

“…[19][20][21][22][23][24][25] Various other analytical methods have also been applied to detect APAP and its metabolites, such as high performance liquid chromatography (HPLC) in combination with accelerator mass spectrometry (AMS), [26] diode-array detection (DAD), [8] chemical reaction interface for mass spectrometry (CRIMS), [27] UV, [9] as well as capillary electrophoresis (CE) with UV detection. [19][20][21][22][23][24][25] Various other analytical methods have also been applied to detect APAP and its metabolites, such as high performance liquid chromatography (HPLC) in combination with accelerator mass spectrometry (AMS), [26] diode-array detection (DAD), [8] chemical reaction interface for mass spectrometry (CRIMS), [27] UV, [9] as well as capillary electrophoresis (CE) with UV detection.…”

Section: Introductionmentioning

confidence: 99%

“…Usually APAP is quantified by immunoassay techniques, either as part of a general screen or as specific target. [19][20][21][22][23][24][25] Various other analytical methods have also been applied to detect APAP and its metabolites, such as high performance liquid chromatography (HPLC) in combination with accelerator mass spectrometry (AMS), [26] diode-array detection (DAD), [8] chemical reaction interface for mass spectrometry (CRIMS), [27] UV, [9] as well as capillary electrophoresis (CE) with UV detection. [28] In particular, hyphenated HPLC-mass spectrometry (LC-MS) has shown great potential, because of its specificity and its ability to identify structurally-related substances that could bias the result of immunoassay analysis.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of acetaminophen and structurally related compounds in human serum and plasma

Bylda

Thiele

Kobold

et al. 2013

Drug Testing and Analysis

View full text Add to dashboard Cite

The method described in this study allows the simultaneous quantification of acetaminophen (APAP) and nine structurally related compounds, namely acetaminophen metabolites and structurally similar analogs (acetaminophen-glucuronide [APG], -sulfate [APS], mercapturate [APM], -cysteine [APC], p-phenetidine, phenacetin), antidote (N-acetylcysteine, NAC), and two tricyclic antidepressants (imipramine and amitryptiline). Due to the relatively high serum concentration levels in the µg/ml range, matrix effects were simply minimized by dilution. The samples were diluted with water and disulfide bonds between serum proteins and analytes reduced using tris(2-carboxyethyl)phosphine. Chromatographic separation of the analytes was achieved by gradient elution using a pentafluorphenyl (PFP) column with subsequent detection by electrospray ionization (ESI) triple quadrupole mass spectrometry in positive and negative ionization multiple reaction monitoring (MRM) modes. Quantification was performed by means of deuterated analogues of the analytes as internal standards. Total run time of the assay was 19 min. The method was fully validated and allowed quantification of the analytes with lower limits of quantification between 50 and 0.5 ng/ml. The calibration curves were linear over the range 0.1-100 µg/ml for APAP, APG, NAC, p-phenetidine and phenacetin, 0.03-50 µg/ml for APS, and 0.01-10 µg/ml for APM, APC, imipramine and amitriptyline with correlation coefficients r(2) > 0.99. The intra-assay precision was ≤5% for all analytes except NAC (CV < 10%). The inter-day precision was ≤10% for all analytes except NAC (inter-assay precision <11%). This method was used to analyze 77 patient and spiked samples and results were consistent with expected values from a round robin test.

show abstract

Simultaneous detection of phenacetin and paracetamol using ELISA and a gold nanoparticle-based immunochromatographic test strip

Yao

Liu

et al. 2021

Analyst

View full text Add to dashboard Cite

show abstract

Highly specific, sensitive and rapid enzyme immunoassays for the measurement of acetaminophen in serum

Cited by 5 publications

References 37 publications

Acetaminophen reduces osteoprotegerin synthesis stimulated by PGE<sub>2</sub> and PGF<sub>2α</sub> in osteoblasts: attenuation of SAPK/JNK but not p38 MAPK or p44/p42 MAPK

Acetaminophen reduces osteoprotegerin synthesis stimulated by PGE<sub>2</sub> and PGF<sub>2α</sub> in osteoblasts: attenuation of SAPK/JNK but not p38 MAPK or p44/p42 MAPK

Simultaneous quantification of acetaminophen and structurally related compounds in human serum and plasma

Simultaneous detection of phenacetin and paracetamol using ELISA and a gold nanoparticle-based immunochromatographic test strip

Contact Info

Product

Resources

About