Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.03.078 View full text
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Lucie Nováková, Tomáš Gottvald, Hana Vlčková, František Trejtnar, Jana Mandíková, Petr Solich

Abstract: Entecavir is a deoxyguanosine nucleotide antiviral agent with the activity against hepatitis B virus (HBV). The agent possesses a polar structure, which is predetermined for hydrophilic interaction chromatography (HILIC). Novel, fast and sensitive HILIC-UHPLC method developed in this study included separation from matrix component on BEH Amide stationary phase by isocratic elution using binary mobile phase composed of acetonitrile/5mM ammonium acetate pH 4.0 (75:25) at flow-rate 0.3 ml/min. Analysis under RP-U…

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