Highly selective fluorescence imaging of zinc distribution in HeLa cells and Arabidopsis using a naphthalene-based fluorescent probe

Lee, Ji Ha; Lee, Jin Hyeok; Jung, Sung Ho; Hyun, Tae Kyung; Feng, Mingxiao; Kim, Jae Yean; Lee, Jae Hong; Lee, Hoyeon; Kim, Jong Seung; Kang, Chulhun; Kwon, Ki Young; Jung, Jong Hwa

doi:10.1039/c5cc01364j

Cited by 53 publications

(11 citation statements)

References 22 publications

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“…Fluorescent probe has been evaluated as the most powerful tool to monitor biologically relevant species for their high sensitivity and spatial resolution (Egawa et al, 2013; Chen et al, 2013; Radford et al, 2013; Huang et al, 2013; Bae et al, 2013; Jung et al, 2014; Zhang et al, 2014; Hettiarachchi et al, 2014; Zhou et al, 2014). During last years, lots of fluorescent probes for Zn 2+ have been developed to detect the cytosolic Zn 2+ to understand its role in living system (Zhang et al, 2014; Hettiarachchi et al, 2014; Zhou et al, 2014; Qian et al, 2009; Du et al, 2010; Meng et al, 2012; Guo et al, 2012; Lin et al, 2013; Divya et al, 2014; Hagimori et al, 2015; Lee et al, 2015). Unfortunately, most of the reported Zn 2+ fluorescent probes failed to target the mitochondria.…”

Section: Introductionmentioning

confidence: 99%

A mitochondria-targeted ratiometric two-photon fluorescent probe for biological zinc ions detection

Ning

Jiang

et al. 2016

Biosensors and Bioelectronics

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A mitochondria-targeted ratiometric two-photon fluorescent probe (Mito-MPVQ) for biological zinc ions detection was developed based on quinolone platform. Mito-MPVQ showed large red shifts (68nm) and selective ratiometric signal upon Zn2+ binding. The ratio of emission intensity (I488 nm/I420 nm) increases dramatically from 0.45 to 3.79 (ca. 8-fold). NMR titration and theoretical calculation confirmed the binding of Mito-MPVQ and Zn2+. Mito-MPVQ also exhibited large two-photon absorption cross sections (150GM) at nearly 720 nm and insensitivity to pH within the biologically relevant pH range. Cell imaging indicated that Mito-MPVQ could efficiently located in mitochondria and monitor mitochondrial Zn2+ under two-photon excitation with low cytotoxicity.

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Section: Introductionmentioning

confidence: 99%

A mitochondria-targeted ratiometric two-photon fluorescent probe for biological zinc ions detection

Ning

Jiang

et al. 2016

Biosensors and Bioelectronics

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“…Although it is not clear how the trace elements are fixed to melanosomes, a metal binding property of melanin in the eye has been well established at least for the following 23 elements: Ag 25 , Al 25 , Ca 25 26 27 , Cr 25 , Cs 25 , Cu 25 28 29 , Fe 25 26 30 , Ga 25 , Hg 25 , K 25 27 , Mg 27 , Mn 25 30 31 , Rb 25 , S 26 , Sr 25 , Ni 25 , Pb 25 , Pd 25 , Pt 25 , Ti 25 , Tl 25 , V 25 , Zn 25 27 28 29 . Previously, zinc ion distribution has been studied using different fluorescent indicators, revealing a punctate cytoplasmic distribution in mouse fibroblasts 11 as well as its localization to the endoplasmic reticulum and mitochondria in HeLa cells 32 . Although no nuclear signal has been detected in this previous study, our μ-XRF images showed zinc signals in the nuclei of retinal cells, in addition to a punctate cytoplasmic signal ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Melanosomes in pigmented epithelia maintain eye lens transparency during zebrafish embryonic development

Takamiya

Suhonen

et al. 2016

Sci Rep

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Altered levels of trace elements are associated with increased oxidative stress that is eventually responsible for pathologic conditions. Oxidative stress has been proposed to be involved in eye diseases, including cataract formation. We visualized the distribution of metals and other trace elements in the eye of zebrafish embryos by micro X-ray fluorescence (μ-XRF) imaging. Many elements showed highest accumulation in the retinal pigment epithelium (RPE) of the zebrafish embryo. Knockdown of the zebrafish brown locus homologues tyrp1a/b eliminated accumulation of these elements in the RPE, indicating that they are bound by mature melanosomes. Furthermore, albino (slc45a2) mutants, which completely lack melanosomes, developed abnormal lens reflections similar to the congenital cataract caused by mutation of the myosin chaperon Unc45b, and an in situ spin trapping assay revealed increased oxidative stress in the lens of albino mutants. Finally transplanting a wildtype lens into an albino mutant background resulted in cataract formation. These data suggest that melanosomes in pigment epithelial cells protect the lens from oxidative stress during embryonic development, likely by buffering trace elements.

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“…Addition of UO 2 2+ in the presence of Ca 2+ and Rb + resulted in a slight fluorescence quenching of 1 at 510 nm; however, other metal ions did not induce such fluorescence quenching effect at 510 nm. In general, UO 2 2+ is known as a hard Lewis acid, such that the nitrogen and oxygen atoms of the N , N ‐bis(acetic acid)aniline moiety of 1 can be effective for selective binding of UO 2 2+ according to the hard soft acid base theory, which were confirmed in the previous studies . These results demonstrated that the fluorescence intensity of probe 1 was effectively enhanced by UO 2 2+ with such a metal ion as the background.…”

Section: Methodsmentioning

confidence: 99%

A Simple Naphthamido‐based Fluorescent Chemoprobe for the Detection of Uranyl Ions

Heo

Choi

Park

et al. 2018

Bulletin Korean Chem Soc

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One of the most important challenges facing the nuclear field is the management of waste generated in the production of nuclear power. Radioactivity occurs inherently in the environment from the past several decades of anthropogenic pursuit including uranium mining, extraction, milling, and nuclear materials production, which has led to the generation and concentration of radioactive materials. 1-3 Heavy metal ions, such as uranium, lead, mercury, and arsenic, are exceptionally toxic elements that can pose a serious risk to human health and the environment. 4-6 Among these, uranium is a significant contaminant of water and soil, which has the most harmful consequence of human health. It can enter into the human body from food, water, and air, and cause serious health problems such as kidney damage, brain injury, and interruption of child's behavior. 7,8 Generally, uranium can exist in four different oxidation states, +3, +4, +5, and +6, but the most relevant form is the linear trans-uranyl ion (UO 2 2+ ). 9,10 Identification and quantification of UO 2 2+ play a significant role in nuclear scientific studies, as this information provides insight into the origin and threat caused by these materials. Therefore, considerable interest has been given to human health and environmental science to improve sensitive methods of selectively detecting and quantifying UO 2 2+ levels in an aqueous solution. Recently, researchers have developed efficient methods for the study of selective and sensitive UO 2 2+ detection, such as atomic emission spectroscopy, 11 total reflection X-ray fluorescence spectrometry, 12 surfaceenhanced Raman spectroscopy, 13 and cathodic stripping voltammetry. 14 However, these techniques include expensive instruments, and sample preparation is tedious and time-consuming. Therefore, it is necessary to develop facile and appropriate techniques for recognizing UO 2 2+ ions in an aqueous environment. In addition, chemical probes for the detection of UO 2 2+ were reported little. Herein, we report the development of a fluorescent probe for the determination of UO 2 2+ ions in the aqueous solution. Among various methods, fluorescence analysis recognizes more attraction due to its extraordinary quantum yield, emission at long wavelengths, high selectivity, good biocompatibility, and stability.

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Highly selective fluorescence imaging of zinc distribution in HeLa cells and Arabidopsis using a naphthalene-based fluorescent probe

Abstract: 2-(N,N-Dimethylamino)naphthalene-based probe 1 was found to exhibit a dramatic enhancement in fluorescence upon addition of Zn(2+), but not with any other metal ions. Probe 1 as a chemoprobe enabled high-resolution fluorescence imaging of zinc ions in HeLa cells and Arabidopsis.

Cited by 53 publications

References 22 publications

A mitochondria-targeted ratiometric two-photon fluorescent probe for biological zinc ions detection

A mitochondria-targeted ratiometric two-photon fluorescent probe for biological zinc ions detection

Melanosomes in pigmented epithelia maintain eye lens transparency during zebrafish embryonic development

A Simple Naphthamido‐based Fluorescent Chemoprobe for the Detection of Uranyl Ions

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