Highly performing graphene-based field effect transistor for the differentiation between mild-moderate-severe myocardial injury

Rodrigues, Teresa; Mishyn, Vladyslav; Leroux, Yann R.; Butruille, Laura; Woitrain, Eloïse; Barras, Alexandre; Aspermair, Patrik; Happy, H.; Kleber, Christoph; Boukherroub, Rabah; Montaigne, David; Knoll, Wolfgang; Szunerits, Sabine

doi:10.1016/j.nantod.2022.101391

Cited by 39 publications

(45 citation statements)

References 32 publications

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“…The total change in current of more than 30% is observed throughout the six orders of magnitude of NT-proBNP concentration. The measurement of NT-proBNP was carried out in 0.1 × PBS and Twin solution to increase the Debye length [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

“…We further applied the model for the Langmuir isotherm for binding between protein from the solution and the aptamer receptor on the sensor surface, suggested in [ 19 ] to calculate the dissociation constant. Surface coverage (Θ) was plotted as:

where I D (C 0 ) is a drain current at concentration C 0 , I D (C ∞ ) is a drain current at an infinite concentration of analyte.…”

Section: Resultsmentioning

confidence: 99%

“…Azide-modified aptamer was dissolved in 1 mL of 1 × PBS to achieve 3 μM at a room temperature [ 19 ]. A 100 µL drop was placed on GFET prior to binding.…”

Section: Methodsmentioning

confidence: 99%

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One-Step Photochemical Immobilization of Aptamer on Graphene for Label-Free Detection of NT-proBNP

et al. 2022

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A novel photochemical technological route for one-step functionalization of a graphene surface with an azide-modified DNA aptamer for biomarkers is developed. The methodology is demonstrated for the functionalization of a DNA aptamer for an N-terminal B-type natriuretic peptide (NT-proBNP) heart failure biomarker on the surface of a graphene channel within a system based on a liquid-gated graphene field effect transistor (GFET). The limit of detection (LOD) of the aptamer-functionalized sensor is 0.01 pg/mL with short response time (75 s) for clinically relevant concentrations of the cardiac biomarker, which could be of relevance for point-of-care (POC) applications. The novel methodology could be applicable for the development of different graphene-based biosensors for fast, stable, real-time, and highly sensitive detection of disease markers.

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Section: Resultsmentioning

confidence: 99%

where I D (C 0 ) is a drain current at concentration C 0 , I D (C ∞ ) is a drain current at an infinite concentration of analyte.…”

Section: Resultsmentioning

confidence: 99%

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One-Step Photochemical Immobilization of Aptamer on Graphene for Label-Free Detection of NT-proBNP

et al. 2022

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“…The major problems persisting for diagnostic use are: (i) maintaining of high charge mobility (on the biosensor) after surface modification with specific receptors to achieve high sensitivity and selectivity; (ii) reproducibility of device preparation, and (iii) limitations due to Debye screening length in biological fluids with high salt concentrations such as blood and serum. Several reports over the last few years have shown that Graphene Field-Effect Transistors (GFETs) stand out for their small size, excellent electrical characteristics, and high sensitivity to near surface charges and electrical fields, making them ideal devices for sensitive sensing [3][4][5][6]. Specificity to the target analyte can be integrated into GFETs via surface attachment of target-specific receptors.…”

Section: Discussionmentioning

confidence: 99%

State of the Art of Chemosensors in a Biomedical Context

et al. 2022

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Healthcare is undergoing large transformations, and it is imperative to leverage new technologies to support the advent of personalized medicine and disease prevention. It is now well accepted that the levels of certain biological molecules found in blood and other bodily fluids, as well as in exhaled breath, are an indication of the onset of many human diseases and reflect the health status of the person. Blood, urine, sweat, or saliva biomarkers can therefore serve in early diagnosis of diseases such as cancer, but also in monitoring disease progression, detecting metabolic disfunctions, and predicting response to a given therapy. For most point-of-care sensors, the requirement that patients themselves can use and apply them is crucial not only regarding the diagnostic part, but also at the sample collection level. This has stimulated the development of such diagnostic approaches for the non-invasive analysis of disease-relevant analytes. Considering these timely efforts, this review article focuses on novel, sensitive, and selective sensing systems for the detection of different endogenous target biomarkers in bodily fluids as well as in exhaled breath, which are associated with human diseases.

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“…The window period provides an opportunity for daily warning of AMI. So far, methods based on electrochemiluminescence, − fluorescence immunoassay, , electrochemistry, − and field-effect transistors − have been reported for the detection of cTnI proteins, but most usually require the involvement of enzymes with catalysis, which exposes their drawbacks such as strong ties to sophisticated instruments, they are time-consuming (>6 h), and they require the involvement of the educated. , Therefore, there is an urgent need to exploit a highly sensitive, reliable, and low-cost immediate test for rapid quantification and monitoring of cTnI protein.…”

mentioning

confidence: 99%

Liposome-Embedded Cu_2–xAg_xS Nanoparticle-Mediated Photothermal Immunoassay for Daily Monitoring of cTnI Protein Using a Portable Thermal Imager

Liu

Tang

2022

Anal. Chem.

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Functional photothermal nanomaterials have gained widespread attention in the field of precise cancer therapy and early disease diagnosis due to their unique photothermal conversion properties. However, the relatively narrow temperature response range and the outputable accuracy of commercial thermometers limit the accurate detection of biomarkers. Herein, we designed a liposome-embedded Cu 2−x Ag x S amplification-based photothermal sensor for the accurate determination of cardiac troponin I (cTnI) in health monitoring and point-of-care testing (POCT). The combinable 3D-printing detecting device monitored and visualized target signal changes in the testing system under the excitation of near-infrared (NIR) light, which was recorded and evaluated for possible pathogenicity by a smartphone. Notably, we predicted the potentially efficient thermal conversion efficiency of Cu 2−x Ag x S from the structure and charge density distribution, calculated by the first-principles and density functional theory (DFT), which provided a theoretical basis for the construction of novel photothermal materials, and the experimental results proved the correctness of the theoretical projections. Under optimal conditions, the photothermal immunoassay showed a dynamic linear range of 0.02−10 ng mL −1 with a detection limit of 11.2 pg mL −1 . This work instructively introduces promising theoretical research and provides new insights for the development of sensitive portable photothermal biosensors.

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Highly performing graphene-based field effect transistor for the differentiation between mild-moderate-severe myocardial injury

Cited by 39 publications

References 32 publications

One-Step Photochemical Immobilization of Aptamer on Graphene for Label-Free Detection of NT-proBNP

One-Step Photochemical Immobilization of Aptamer on Graphene for Label-Free Detection of NT-proBNP

State of the Art of Chemosensors in a Biomedical Context

Liposome-Embedded Cu_2–xAg_xS Nanoparticle-Mediated Photothermal Immunoassay for Daily Monitoring of cTnI Protein Using a Portable Thermal Imager

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Highly performing graphene-based field effect transistor for the differentiation between mild-moderate-severe myocardial injury

Cited by 39 publications

References 32 publications

One-Step Photochemical Immobilization of Aptamer on Graphene for Label-Free Detection of NT-proBNP

One-Step Photochemical Immobilization of Aptamer on Graphene for Label-Free Detection of NT-proBNP

State of the Art of Chemosensors in a Biomedical Context

Liposome-Embedded Cu2–xAgxS Nanoparticle-Mediated Photothermal Immunoassay for Daily Monitoring of cTnI Protein Using a Portable Thermal Imager

Contact Info

Product

Resources

About

Liposome-Embedded Cu_2–xAg_xS Nanoparticle-Mediated Photothermal Immunoassay for Daily Monitoring of cTnI Protein Using a Portable Thermal Imager