Higher genetic diversity and gene flow in wild populations of Miscanthus sinensis in southwest China

Xu, Weifeng; Zhang, Xinquan; Huang, Linkai; Nie, Gang; Wang, Jianping

doi:10.1016/j.bse.2012.11.024

Cited by 18 publications

(27 citation statements)

References 28 publications

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“…That stays in agreement with a number of other studies [13, 17, 29, 36, 61]. Taking into consideration the UPGMA analysis we assigned three subclusters in M. sinensis cluster.…”

Section: Discussionsupporting

confidence: 92%

Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers

2014

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Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker systems such as: inter simple sequence repeats (ISSRs), random amplified polymorphic DNA (RAPD), and by estimation of ploidy level based on flow cytometry. As a result, only one ISSR primer (ISSR1) and three RAPD primers (RAPD1, RAPD2, RAPD4) were required to identify all genotypes. Moreover, the use of the above mentioned molecular markers enable the proper species recognition of the interspecific hybrid M. × giganteus “Floridulus,” which has been previously mislabeled as M. floridulus. The highest genetic similarity coefficient (0.94) was observed between M. × giganteus clones, which indicates that the genetic diversity within this species was very low. Whereas M. sinensis genotypes represented a relatively wide diversity with similarity coefficient of 0.58. Cluster analysis using UPGMA grouped the 18 accessions in three clusters according to species affiliation including relabeled M. × giganteus “Floridulus,” which proved to be closely related to M. × giganteus. Similar groupings were evident in the PCoA analysis.

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“…That stays in agreement with a number of other studies [13, 17, 29, 36, 61]. Taking into consideration the UPGMA analysis we assigned three subclusters in M. sinensis cluster.…”

Section: Discussionsupporting

confidence: 92%

Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers

2014

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“…Although these large number of SSRs have been successfully applied to M. sinensis studies and proven with high transferability (Hernandez et al, 2001; Zhao et al, 2011; Xu et al, 2013; Chae et al, 2014; Yook et al, 2014; Nie et al, 2016), the specificity and function of markers seems less than satisfactory. The SSRs developed from Miscanthus transcriptome under drought conditions were aimed to the function regions among expressed unigenes, which could be facilitate better discovering of the marker-trait associations and higher efficiency.…”

Section: Resultsmentioning

confidence: 99%

Development of SSR Markers Based on Transcriptome Sequencing and Association Analysis with Drought Tolerance in Perennial Grass Miscanthus from China

et al. 2017

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Drought has become a critical environmental stress affecting on plant in temperate area. As one of the promising bio-energy crops to sustainable biomass production, the genus Miscanthus has been widely studied around the world. However, the most widely used hybrid cultivar among this genus, Miscanthus × giganteus is proved poor drought tolerance compared to some parental species. Here we mainly focused on Miscanthus sinensis, which is one of the progenitors of M. × giganteus providing a comparable yield and well abiotic stress tolerance in some places. The main objectives were to characterize the physiological and photosynthetic respond to drought stress and to develop simple sequence repeats (SSRs) markers associated with drought tolerance by transcriptome sequencing within an originally collection of 44 Miscanthus genotypes from southwest China. Significant phenotypic differences were observed among genotypes, and the average of leaf relative water content (RWC) were severely affected by drought stress decreasing from 88.27 to 43.21%, which could well contribute to separating the drought resistant and drought sensitive genotype of Miscanthus. Furthermore, a total of 16,566 gene-associated SSRs markers were identified based on Illumina RNA sequencing under drought conditions, and 93 of them were randomly selected to validate. In total, 70 (75.3%) SSRs were successfully amplified and the generated loci from 30 polymorphic SSRs were used to estimate the genetic differentiation and population structure. Finally, two optimum subgroups of the population were determined by structure analysis and based on association analysis, seven significant associations were identified including two markers with leaf RWC and five markers with photosynthetic traits. With the rich sequencing resources annotation, such associations would serve an efficient tool for Miscanthus drought response mechanism study and facilitate genetic improvement of drought resistant for this species.

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“…Arnoult and Brancourt-Hulmel (2014) reviewed the high genotypic diversity among and within the three most studied Miscanthus species (M. × giganteus, M. sacchariflorus, and M. sinensis). In a comparison of 26 wild populations of M. sinensis from southwest China, Xu et al (2013) even found a higher genetic diversity within each population than among populations. These findings indicate a good potential of miscanthus for plant breeding to maximize cellulosic ethanol production and optimize the conversion process.…”

Section: Impact On Genetic Improvementmentioning

confidence: 94%

Miscanthus clones for cellulosic bioethanol production: Relationships between biomass production, biomass production components, and biomass chemical composition

Arnoult¹,

Obeuf²,

Béthencourt³

et al. 2015

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Higher genetic diversity and gene flow in wild populations of Miscanthus sinensis in southwest China

Cited by 18 publications

References 28 publications

Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers

Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers

Development of SSR Markers Based on Transcriptome Sequencing and Association Analysis with Drought Tolerance in Perennial Grass Miscanthus from China

Miscanthus clones for cellulosic bioethanol production: Relationships between biomass production, biomass production components, and biomass chemical composition

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