High titer ethanol production from simultaneous enzymatic saccharification and fermentation of aspen at high solids: A comparison between SPORL and dilute acid pretreatments

Zhu, Jiaqing; Gleisner, Roland; Scott, C. Tim; Luo, Xiaolin; Tian, Shen

doi:10.1016/j.biortech.2011.07.047

Cited by 73 publications

(49 citation statements)

References 30 publications

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“…However, ethanol yield of FBP-SSF at 20% solid was very low comparing to ethanol production of Zhu et al [15], which could produce ethanol from aspen up to 133 L/ton DM. To increase ethanol yield, research and development of ethanol production by FBP-SSF should be therefore done further study.…”

Section: Effect Of Commercial Cellulase Enzymes On Ethanol Productionmentioning

confidence: 77%

“…Su et al [5] also used prehydrolysis at 50 °C for 24 h before ethanol production by simultaneous saccharification and co-fermentation (SSCF). Zhu et al [15] used liquefaction at 50 °C for 120 h before ethanol production by SSF. Although it could give high ethanol concentration over the benchmark level (above 4.0% (v/v)) [5,16,17], it might be economically viable to produce in large scale.…”

Section: Effect Of Commercial Cellulase Enzymes On Ethanol Productionmentioning

confidence: 99%

“…Although it could give high ethanol concentration over the benchmark level (above 4.0% (v/v)) [5,16,17], it might be economically viable to produce in large scale. However, almost of material pretreatment was used in high severity condition, especially high temperature, such as Chu et al [9] pretreated corn stover at 190 °C for 3 min, Zhu et al [15] pretreated aspen at 170 °C for 10 min, and Albuquerque-Wanderley et al [18] pretreated sugarcane bagasse at 200 °C for 7 min. In addition, the mixing with higher efficiency than incubator shaker was used, such as Chu et al [9] used helical stirring for mixing and fermented by thermotolerant yeast strain S. cerevisiae DQ1 at 40 °C.…”

Section: Effect Of Commercial Cellulase Enzymes On Ethanol Productionmentioning

confidence: 99%

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Effect of Commercial Cellulase Enzymes on Ethanol Production from Pretreated Rice Straw at High Solid Loading

Srinorakutara¹,

Subkaree²,

Boonvitthya³

et al. 2015

JFSE

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Effect of commercial cellulose enzymes was investigated by batch enzymatic hydrolysis at 15.0% (w/v) solid. It was found that the best commercial cellulose enzyme was Cellic ® CTec comparing to Accellerase 1000 TM and Accelerase 1500 TM . The Cellic ® CTec gave the highest reducing sugar concentration and rice straw conversion. Moreover, when the hydrolysate obtained from hydrolysis using Cellic ® CTec was fermented by Saccharomyces cerevisiae TISTR 5596, it would give the highest ethanol. In this study, the Cellic ® CTec was used for fed-batch prehydrolysis prior to ethanol production by simultaneous saccharification and fermentation (SSF) way at 20% (w/v) solid loading. It could produce 35.76 g/L or 4.6% (v/v) of ethanol concentration and 83.67 L/ton dry matter (DM) of yield.

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Section: Effect Of Commercial Cellulase Enzymes On Ethanol Productionmentioning

confidence: 77%

Section: Effect Of Commercial Cellulase Enzymes On Ethanol Productionmentioning

confidence: 99%

Section: Effect Of Commercial Cellulase Enzymes On Ethanol Productionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Commercial Cellulase Enzymes on Ethanol Production from Pretreated Rice Straw at High Solid Loading

Srinorakutara¹,

Subkaree²,

Boonvitthya³

et al. 2015

JFSE

View full text Add to dashboard Cite

show abstract

“…The sugar content of extract solutions (in g l -1 ), aft er fi ltering and acid hydrolysis, was determined by HPLC with pulsed amperometric detection (Davis 1998 ). The chemical composition of the raw material was 45.6 % glucan, 16.3 % xylan, 1.41 % mannan, 0.35 % arabinan, and 0.46 % galactan (Zhu et al 2011 ).…”

Section: Methodsmentioning

confidence: 99%

Time-domain NMR study of the drying of hemicellulose extracted aspen (Populus tremuloides Michx.)

Elder

Houtman

2012

Holzforschung

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The effect of hot water on aspen chips has been evaluated using time-domain low-field nuclear magnetic resonance (NMR) spectroscopy. At moisture contents above fiber saturation point, treated chips exhibit relaxation times of free water longer than for the control. This is consistent with the removal of hemicelluloses given the hydrophilicity of these polysaccharides. The rate of decrease in free water upon drying is greater for the pretreated samples. These results indicate that hot water treatment modifies both the chemical and physical properties of the chips and may therefore impact product manufacture and performance.

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“…Sulfite pretreatment to overcome the recalcitrance of lignocelluloses (SPORL) (Zhu et al, 2009) has demonstrated robust performance for high titer and high yield ethanol production from softwood biomass without detoxification (Zhou et al, 2013b(Zhou et al, , 2015Zhu et al, 2015). Building upon the success of SPORL, we demonstrated SPORL optimization for bioconversion of a poplar wood in laboratory bench scale (1 L) experiments using a combined hydrolysis factor (CHF) to control pretreatment severity at CHF ≈ 2, which allowed us to successfully achieve high titer (>40 g L −1 ) ethanol production without detoxification using an engineered Saccharomyces cerevisiae (Zhang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

High Titer Ethanol and Lignosulfonate Production from SPORL Pretreated Poplar at Pilot Scale

et al. 2015

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Poplar NE222 (Populus deltoides Bartr. ex Marsh × P. nigra L.) wood chips were pretreated in a 390 L pilot-scale rotating wood-pulping digester using a dilute sulfite solution of approximately pH 1.8 at 160°C for 40 min for bioconversion to ethanol and lignosulfonate (LS). An estimated combined hydrolysis factor (CHF) of 3.3 was used to scale the sulfite pretreatment temperature and time from laboratory bench scale experiments, which balanced sugar yield and inhibitor formation to facilitate high titer ethanol production through fermentation using S. cerevisiae YRH400 without detoxification. A terminal ethanol titer of 43.6 g L -1 with a yield of 247 L tonne wood -1 was achieved at total solids loading of 20%. The relatively low ethanol yield compared with yield from Sulfite pretreatment to overcome the recalcitrance of lignocelluloses (SPORL)-pretreated softwoods was due to inefficient utilization of xylose. The LS from SPORL has a substantially higher phenolic group (Ph-OH) content, though it was less sulfonated and had a lower molecular weight than a purified commercial softwood LS, and therefore has potential for certain commercial markets and future novel applications through further processing. The conversion efficiency achieved through process integration and simplification, demonstrated here, has significant importance to the entire supply chain of biofuel production from woody biomass.

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High titer ethanol production from simultaneous enzymatic saccharification and fermentation of aspen at high solids: A comparison between SPORL and dilute acid pretreatments

Cited by 73 publications

References 30 publications

Effect of Commercial Cellulase Enzymes on Ethanol Production from Pretreated Rice Straw at High Solid Loading

Effect of Commercial Cellulase Enzymes on Ethanol Production from Pretreated Rice Straw at High Solid Loading

Time-domain NMR study of the drying of hemicellulose extracted aspen (Populus tremuloides Michx.)

High Titer Ethanol and Lignosulfonate Production from SPORL Pretreated Poplar at Pilot Scale

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