High Throughput Screening System for screening of 3D cell cultures

Gallert, C.; Lehmann, R.; Roddelkopf, Thomas; Junginger, Steffen; Thurow, Kerstin

doi:10.1109/i2mtc.2015.7151461

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…The processes and devices are manageable by the Biomek software and SAMI software. 29 EZ4U Cell Proliferation Assay. The EZ4U assay kit (Biomedica, Vienna, Austria) was used to evaluate the cell proliferation.…”

Section: Screening Processesmentioning

confidence: 99%

Biomek Cell Workstation: A Flexible System for Automated 3D Cell Cultivation

et al. 2016

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The shift from 2D cultures to 3D cultures enables improvement in cell culture research due to better mimicking of in vivo cell behavior and environmental conditions. Different cell lines and applications require altered 3D constructs. The automation of the manufacturing and screening processes can advance the charge stability, quality, repeatability, and precision. In this study we integrated the automated production of three 3D cell constructs (alginate beads, spheroid cultures, pellet cultures) using the Biomek Cell Workstation and compared them with the traditional manual methods and their consequent bioscreening processes (proliferation, toxicity; days 14 and 35) using a high-throughput screening system. Moreover, the possible influence of antibiotics (penicillin/streptomycin) on the production and screening processes was investigated. The cytotoxicity of automatically produced 3D cell cultures (with and without antibiotics) was mainly decreased. The proliferation showed mainly similar or increased results for the automatically produced 3D constructs. We concluded that the traditional manual methods can be replaced by the automated processes. Furthermore, the formation, cultivation, and screenings can be performed without antibiotics to prevent possible effects.

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Section: Screening Processesmentioning

confidence: 99%

Biomek Cell Workstation: A Flexible System for Automated 3D Cell Cultivation

et al. 2016

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“…High-throughput assays have become an indispensable tool for modern biotechnology and biology [1]- [3] since they enable generating, analysis, and processing of a large amount of data. Using assays, overall experimental run times can be significantly reduced while improving data reliability and reproducibility by eliminating human error.…”

Section: Introductionmentioning

confidence: 99%

Grid Screener: A Tool for Automated High-Throughput Screening on Biochemical and Biological Analysis Platforms

Schilling¹,

Schmelzer

Gómez

et al. 2021

IEEE Access

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Grid structures are common in high-throughput assays to parallelize experiments in biochemical or biological experiments. Manual analysis of grid images is laborious, time-consuming, expensive, and critical in terms of reproducibility. However, it is still common to do such analysis manually, as there is no standardized software for automated analysis. In this paper, we introduce a generic method to automatically detect grid structures in images and to perform flexible spot-wise analysis after successful grid detection. The deep learning-based approach of the grid structure detection allows being flexible concerning different grid types. The combination with a robust parameter estimation algorithm lowers the requirements of the detection quality and thus enhances robustness. Further, the method conducts semi-automated grid detection if a fully automated processing fails. An open-source software tool Grid Screener that implements the proposed methods is provided as a ready-for-use tool for researchers. The usability is demonstrated by taking different criteria into account, which are important for a successful application. We present the benefits of our proposed tool Grid Screener utilizing three different grid types in the context of high-throughput screening to show our contribution towards further lab automation. Our tool performs much faster than manual analysis, while maintaining or even enhancing accuracy.

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High Throughput Screening System for screening of 3D cell cultures

Cited by 2 publications

References 3 publications

Biomek Cell Workstation: A Flexible System for Automated 3D Cell Cultivation

Biomek Cell Workstation: A Flexible System for Automated 3D Cell Cultivation

Grid Screener: A Tool for Automated High-Throughput Screening on Biochemical and Biological Analysis Platforms

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