High-throughput phenotyping of plant resistance to aphids by automated video tracking

Kloth, Karen J.; Broeke, C; Thoen, Manus P. M.; Brink, Marianne Hanhart-van den; Wiegers, G.L.; Krips, O.E.; Noldus, L.P.J.J.; Dicke, Marcel; Jongsma, Maarten A.

doi:10.1186/s13007-015-0044-z

Cited by 32 publications

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“…In such trials, multiple stresses can occur, but their occurrence and the intensity with which they occur are not guaranteed and, therefore, plant breeders developed the concept of managed stress trials in which specific and welldefined stress conditions are imposed for a single stress or a small number of stresses (Cooper & Hammer, 1996;Cooper et al, 2014). Recently, the urge to manage environmental factors even more precisely has led to the development of phenotyping platforms, where, again, mainly single stresses are investigated (Fiorani & Schurr, 2013;Granier & Vile, 2014;Kloth et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Genetic architecture of plant stress resistance: multi‐trait genome‐wide association mapping

et al. 2016

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Summary Plants are exposed to combinations of various biotic and abiotic stresses, but stress responses are usually investigated for single stresses only.Here, we investigated the genetic architecture underlying plant responses to 11 single stresses and several of their combinations by phenotyping 350 Arabidopsis thaliana accessions. A set of 214 000 single nucleotide polymorphisms (SNPs) was screened for marker‐trait associations in genome‐wide association (GWA) analyses using tailored multi‐trait mixed models.Stress responses that share phytohormonal signaling pathways also share genetic architecture underlying these responses. After removing the effects of general robustness, for the 30 most significant SNPs, average quantitative trait locus (QTL) effect sizes were larger for dual stresses than for single stresses.Plants appear to deploy broad‐spectrum defensive mechanisms influencing multiple traits in response to combined stresses. Association analyses identified QTLs with contrasting and with similar responses to biotic vs abiotic stresses, and below‐ground vs above‐ground stresses. Our approach allowed for an unprecedented comprehensive genetic analysis of how plants deal with a wide spectrum of stress conditions.

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Section: Introductionmentioning

confidence: 99%

Genetic architecture of plant stress resistance: multi‐trait genome‐wide association mapping

et al. 2016

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“…Although the duration of time spent in a specific zone showed a significant difference (choice parameter) for the two accessions tested, the highest levels of significance were found in the parameters "duration not moving" and "proportion of time moving", indicating that the actual behavior exhibited on a specific leaf disc harbors variables that can be used to phenotype plants for thrips resistance more accurately. A potentially interesting next step would be to also discriminate between short and long non-movement events (Kloth, ten Broeke et al 2015). Short non-movement events could refer to test probes, nonmovement events that last longer than 10 seconds are potential food uptake events.…”

Section: Discussionmentioning

confidence: 99%

“…Some progress has recently been made in phenotyping plant resistance to pest insects (e.g. hemipterans (Kloth, ten Broeke et al 2015) and lepidopterans (Green, Appel et al 2012)). However, no efficient systems have been developed to study host-plant resistance to thrips.…”

Section: Introductionmentioning

confidence: 99%

“…This study describes automated video tracking of thrips behavior as a method to assess host-plant resistance, which can complement end-point analysis. Automated video tracking of animal behavior was introduced in the early 1990s (Bakchine, Pham-Delegue et al 1990, Noldus, Spink et al 2002, but not applied until recently to the study of host-plant resistance to herbivorous insects (Kloth, ten Broeke et al 2015). Previously, we demonstrated the value of video tracking aphid behavior in non-choice assays, assessing host-plant resistance in Arabidopsis thaliana and lettuce against Myzus persicae and Nasonovia ribisnigri respectively (Kloth, ten Broeke et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Host-plant resistance to western flower thrips in Arabidopsis

Thoen

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The fact that plants are sessile organisms does by no means imply that they are helpless victims. Although they are harassed in some shape or form by members of all kingdoms of life, it is exactly this harassment that has shaped and defined the unique defensive arsenal of plants. Plants have evolved morphological features that hinder herbivory, like trichomes and leaf waxes (Panda and Khush 1995, Schoonhoven, van Loon et al. 2005), but likely even more important are the diverse chemical defenses (Schoonhoven, van Loon et al. 2005(Mithöfer and Boland 2012) that have evolved under the selection pressure of herbivores. Frankliniella occidentalis is often studied in relation to economically relevant (ornamental) crops, and some of these studies have revealed chemical resistance in plants against thrips herbivory. In chrysanthemum (Dendranthema grandiflora) using TLC and HPLC, isobutylamide was proposed as a candidate compound involved in thrips resistance (Tsao, Marvin et al. 2005). A different study, using nuclear magnetic resonance (NMR)-based metabolomics showed that thrips-resistant chrysanthemum varieties contained higher amounts of chlorogenic acid and additional bioassays with artificial diets confirmed the negative effect of this phenylpropanoid on the survival rate of juveniles (Leiss, Maltese et al. 2009). Although chlorogenic acid occurs in high levels in carrot plants (Daucus carota L.), NMR did not show differences in cholorogenic levels between resistant and susceptible carrots. Instead, the flavonoid luteolin, the phenylpropanoid sinapic acid and the amino acid β-alanine were correlated with thrips resistance in this plant species (Leiss, Cristofori et al. 2013). Using several wild and cultivated tomato species, the presence of acylsugars was correlated with lowered thrips feeding damage (Mirnezhad, Romero-Gonzalez et al. 2010). Using LC-MS on nine different pepper (Capsicum) cultivars showed a correlation with several tocopherols, alkanes, a sterol and a terpene in relation to thrips resistance, although no bioassays were done to confirm the activity of these compounds (Awang 2013). Studies with the model plant Arabidopsis thaliana using transcriptomics and different knockout mutants, revealed a prominent role for the phytohormone jasmonic acid (JA) in thrips resistance. Mutant plants hampered in their JA signaling pathway, were not able to trigger induced defenses leading to higher susceptibility in two-choice assays (De Vos, Van Oosten et al. 2005). The function of JA in thrips resistance is not restricted to Arabidopsis but is likely a general mechanism underlying resistance of plants to thrips. In Chinese cabbage (Brassica rapa), JA also played a crucial role in the induced defenses triggered by F. occidentalis (Abe, Shimoda et al. 2009). Depending on the plant species and analytic tools used, different candidate compounds have been brought forward that play a potential role in thrips resistance. This is perhaps not surprising, given the polyphagous nature of F. occidentalis. Correlations between ...

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“…Genotypic means were calculated with Genstat 15th edition (Payne, 2009), using the following mixed model: (Kloth et al, 2015). Aphid survival was checked 24 hours after recording.…”

Section: Genotypic Meansmentioning

confidence: 99%

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Supplementary Data : Genetic Architecture of Plant Stress Resistance: Multi-Trait Genome-Wide Association Mapping

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High-throughput phenotyping of plant resistance to aphids by automated video tracking

Cited by 32 publications

References 47 publications

Genetic architecture of plant stress resistance: multi‐trait genome‐wide association mapping

Genetic architecture of plant stress resistance: multi‐trait genome‐wide association mapping

Host-plant resistance to western flower thrips in Arabidopsis

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