High throughput liquid chromatography with sub-2μm particles at high pressure and high temperature

Nguyen, Dao T.-T.; Guillarme, Davy; Heinisch, Sabine; Barrioulet, M.; Rocca, Jean‐Louis; Rudaz, Serge; Veuthey, Jean‐Luc

doi:10.1016/j.chroma.2007.08.032

Cited by 115 publications

(70 citation statements)

References 41 publications

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“…To meet these demands, manufacturers of HPLC equipment and consumables have developed and introduced a multitude of stationary phases and columns geometries, along with new instrumental setups. This has not been accompanied by corresponding developments in phase chemistries; RP columns still represent the workhorses in HPLC, and few truly new separation chemistries have emerged [1,2]. Different modifications have been made, such as embedding of polar groups by end-capping or as part of the hydrophobic chain, yet still certain groups of solutes like polar and hydrophilic compounds are problematic to retain and detect sensitively under RP conditions.…”

Section: Introductionmentioning

confidence: 99%

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Fast hydrophilic interaction liquid chromatographic separations on bonded zwitterionic stationary phase

Appelblad¹,

Jonsson²,

Jiang³

et al. 2008

J of Separation Science

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Separation science is an art of obtaining adequate resolution of the desired compounds in minimum time, and with minimum effort in terms of sample preparation and data evaluation. In LC, where selectivity is a main driving force for separation, the availability of different separation modes capable of operating at high flow rates is a way to make combined optimal use of selectivity, efficiency, and speed. The separation of polar and hydrophilic compounds is problematic in RP LC due to the poor retention. Hydrophilic interaction liquid chromatography (HILIC) is a more straightforward separation mode to address this problem. Herein, it is shown that separations in HILIC mode are equally efficient as for RP, providing a potential for very fast separations on short columns. This is not only facilitated by the low viscosity of the mobile phase compositions used, compared to typical RP eluents, but also due to higher column permeability. To exemplify this, baseline separations of uracil and cytosine are shown in less than 4 s and of Tamiflu and its main metabolite in less than 40 s, both under isocratic conditions. HILIC must therefore be considered having potential for high throughput purposes, and being an attractive candidate as the second separation dimension in 2-D HPLC.

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Section: Introductionmentioning

confidence: 99%

“…Improved mass transfer is another benefit at elevated temperatures, as both the dielectric constant and water surface tension decrease with temperature. Worth noting is that resolution and selectivity for certain solutes can also be modified with temperature [2,5].…”

Section: Introductionmentioning

confidence: 99%

Fast hydrophilic interaction liquid chromatographic separations on bonded zwitterionic stationary phase

Appelblad¹,

Jonsson²,

Jiang³

et al. 2008

J of Separation Science

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show abstract

“…A diminuição do tamanho da partícula provoca um aumento na pressão do sistema, conforme a Equação 2: 6,15 (2) onde P = pressão, φ = resistência à vazão, L = comprimento da coluna (mm), η = viscosidade da FM (mPa/s), µ = velocidade linear (mm/s) e d p = tamanho da partícula (µm).…”

Section: Influência Do Tamanho Das Partículas Em Cromatografia Líquidaunclassified

O estado da arte da cromatografia líquida de ultra eficiência

Maldaner

Jardim

2009

Quím. Nova

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Recebido em 8/4/08; aceito em 14/7/08; publicado na web 5/12/08 THE STATE OF ART OF ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY. Ultra Performance Liquid Chromatography (UPLC) retains the same principles as High Performance Liquid Chromatography (HPLC), but uses 1-2.1 mm i.d. columns with sub-2 µm particles. It is considered the newest advance in analytical separation science. The use of these small particles with mobile phases at high linear velocities increases resolution and detectability and decreases analysis time. Thus, the analyses are faster, the solvent volume is smaller, the efficiency is higher and the detectability is 2-3 times higher when compared with HPLC analysis.

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“…Similarly, high-temperature liquid chromatography (HTLC) is a promising technology that utilizes temperatures up to several hundred centigrades in order to decrease the high back pressure formed by high flow rates [5]. Combining high temperature and UHPLC is thus logical and has been applied already with good success [3,4,6,7].…”

Section: Introductionmentioning

confidence: 99%

Ultra high performance liquid chromatography–mass spectrometric analysis of oxidized free fatty acids and acylglycerols

Tarvainen

Suomela

Kallio

2011

Euro J Lipid Sci & Tech

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A fast ultra high performance liquid chromatography (UHPLC)-electrospray ionization (ESI)-mass spectrometric (MS) method was developed for simultaneous analysis of free fatty acids (FFAs), monoacylglycerols (MAG), diacylglycerols (DAG), triacylglycerols (TAG), and their oxidized equivalents. Effect of elevated column temperature was studied in order to optimize the chromatography of closely eluting peaks and to reduce high back pressure formed in UHPLC. The elevated temperature enabled high flow rate, better mass transfer, and therefore more narrow peaks and better separation of the analytes. The new method was applied to the analysis of total lipid extracts of lipolysis samples prepared by an artificial digestion model in order to investigate oxidized lipids and changes in their profiles in the chyme. Over 150 compounds were identified from the extracts. The UHPLC-ESI-MS method was proved to be fast, highly selective, and sensitive. Compared to a previously used high performance LC-ESI-MS method, the new UHPLC-ESI-MS method was over five times faster and consumed one tenth of the solvents while producing comparable quantitative results.Practical applications: Edible oils and fats contain mainly TAGs, the lipolysis of which produces FFAs and MAGs with minor DAG components. These compounds are susceptible to oxidation in the stomach, and therefore the analysis of the oxidation products is important. Fast determination of FFAs and acylglycerols is also important in quality control of biodiesel. Our new method enables accurate and sensitive determination of different molecular species present in digested and processed samples with minimal sample preparation requirements. In this respect, the new method is applicable to large scale and fast screening of biological samples for lipidomic and metabolomic studies.

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High throughput liquid chromatography with sub-2μm particles at high pressure and high temperature

Cited by 115 publications

References 41 publications

Fast hydrophilic interaction liquid chromatographic separations on bonded zwitterionic stationary phase

Fast hydrophilic interaction liquid chromatographic separations on bonded zwitterionic stationary phase

O estado da arte da cromatografia líquida de ultra eficiência

Ultra high performance liquid chromatography–mass spectrometric analysis of oxidized free fatty acids and acylglycerols

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