High-Throughput Glycomic Methods

Trbojević‐Akmačić, Irena; Lageveen-Kammeijer, Guinevere S. M.; Heijs, Bram; Petrović, Tea; Deriš, Helena; Wuhrer, Manfred; Lauc, Gordan

doi:10.1021/acs.chemrev.1c01031

Cited by 37 publications

(23 citation statements)

References 434 publications

(742 reference statements)

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“…In this technique, lectins are immobilized on a solid surface, and binding of target glycoproteins can be detected either directly through their prior labeling with fluorescent reagents, or indirectly by overlaying a fluorescently labeled relevant antibody raised against the target glycoprotein (or via biotinylated antibody and fluorescently labeled streptavidin). The microarray is scanned, followed by the interpretation of the signals [ 71 , 72 ], and structural information about the glycome is obtained using the known glycan-binding specificities of the lectins [ 71 ]. The utilization of multiple lectins in the microarray allows the studying of multiple lectin–glycan interactions in a single experiment and holds the promise of enabling glycomic profiling of cancers in a fast and efficient manner [ 73 ].…”

Section: Lectins As Decoders Of Biological Information In Cellular Gl...mentioning

confidence: 99%

The Blessed Union of Glycobiology and Immunology: A Marriage That Worked

et al. 2023

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In this article, we discuss the main aspects regarding the recognition of cell surface glycoconjugates and the immunomodulation of responses against the progression of certain pathologies, such as cancer and infectious diseases. In the first part, we talk about different aspects of glycoconjugates and delve deeper into the importance of N-glycans in cancer immunotherapy. Then, we describe two important lectin families that have been very well studied in the last 20 years. Examples include the sialic acid-binding immunoglobulin (Ig)-like lectins (siglecs), and galectins. Finally, we discuss a topic that needs to be better addressed in the field of glycoimmunology: the impact of oncofetal antigens on the cells of the immune system. New findings in this area are of great importance for advancement, especially in the field of oncology, since it is already known that cellular interactions mediated by carbohydrate–carbohydrate and/or carbohydrate proteins are able to modulate the progression of different types of cancer in events that compromise the functionality of the immune responses.

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Section: Lectins As Decoders Of Biological Information In Cellular Gl...mentioning

confidence: 99%

The Blessed Union of Glycobiology and Immunology: A Marriage That Worked

et al. 2023

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“…Another key point regarding data collection is the current limited availability of quantitative data that would allow more accurate profiling. At present, immunoglobulin profiling is by far the most advanced in comparison to other glycoconjugates. , Other prospects can be expected to expand from improved techniques in glycan and tissue imaging. , …”

Section: Gradual Impact On Glycoscience and Development Prospectsmentioning

confidence: 99%

“…At present, immunoglobulin profiling is by far the most advanced in comparison to other glycoconjugates. 148,149 Other prospects can be expected to expand from improved techniques in glycan and tissue imaging. 150,151 6.1.2.…”

Section: Expected Evolution Of Ai In Glycoinformaticsmentioning

confidence: 99%

Glycoinformatics in the Artificial Intelligence Era

Bojar

Lisacek

2022

Chem. Rev.

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Artificial intelligence (AI) methods have been and are now being increasingly integrated in prediction software implemented in bioinformatics and its glycoscience branch known as glycoinformatics. AI techniques have evolved in the past decades, and their applications in glycoscience are not yet widespread. This limited use is partly explained by the peculiarities of glyco-data that are notoriously hard to produce and analyze. Nonetheless, as time goes, the accumulation of glycomics, glycoproteomics, and glycan-binding data has reached a point where even the most recent deep learning methods can provide predictors with good performance. We discuss the historical development of the application of various AI methods in the broader field of glycoinformatics. A particular focus is placed on shining a light on challenges in glyco-data handling, contextualized by lessons learnt from related disciplines. Ending on the discussion of state-of-the-art deep learning approaches in glycoinformatics, we also envision the future of glycoinformatics, including development that need to occur in order to truly unleash the capabilities of glycoscience in the systems biology era.

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“…With regard to the role of SARS-CoV-2 glycosylation in viral replication, infectivity and immune response, glycosylation has major potential implications for therapeutic and vaccination strategies as well as serological testing [23]. Historically, the role of glycosylation in the dichotomous immune system has always been the focus of research [24][25][26][27][28][29].…”

Section: Sars-cov-2 and Other Respiratory Viruses Oftenmentioning

confidence: 99%

Importance of glycosylation for SARS-CoV-2 virus, antibodies and COVID-19

Tsamaloukas¹

2022

JCMR

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Summary In addition to the known endemic respiratory viruses, the pandemic SARS-CoV-2 has been added, with terrible effects on the population worldwide. This virus, like many others, has a glycoprotein structure the so-called spike protein, which interacts with the ACE-2 receptor as a cellular entry gate and thus initiates the infection. Just as this glycosylation causes infectiousness, the effect of the protective antibodies is also determined by their degree of glycosylation. In particular, the so-called Fc part of the immunoglobulins plays an important role. The type of bound sugar such as fucose, mannose, sialic acid, etc. determines, among other things, which reactions are triggered by binding to immune cells such as NK cells to their existing Fc receptors. These include antibody-dependent cellular phagocytosis (ADCP), antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent cellular trogocytosis (ADCT) and antibody-dependent cellular complement deposition (ADCD). In addition to protective phagocytosis, these reactions can trigger the antibody-dependent enhancement (ADE) of the infection and also the dreaded cytokine storm in COVID-19 patients. The functional diversification of IgGs by Fc glycosylation can be determined and tracked both after vaccination and during the disease.

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High-Throughput Glycomic Methods

Cited by 37 publications

References 434 publications

The Blessed Union of Glycobiology and Immunology: A Marriage That Worked

The Blessed Union of Glycobiology and Immunology: A Marriage That Worked

Glycoinformatics in the Artificial Intelligence Era

Importance of glycosylation for SARS-CoV-2 virus, antibodies and COVID-19

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