High throughput determination of ammonium and primary amine compounds in environmental and food samples

Robert‐Peillard, Fabien; Palacio, Edwin; Ciulu, Marco; Demelas, Carine; Théraulaz, F.; Boudenne, Jean‐Luc; Coulomb, Bruno

doi:10.1016/j.microc.2017.03.048

Cited by 8 publications

(7 citation statements)

References 23 publications

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“…The preservation of OPA/NAC reagent has already been studied previously [ 35 ]. It had been shown that OPA and NAC solutions were stable for up to four months, provided these solutions were stored separately at 4 °C in the dark.…”

Section: Resultsmentioning

confidence: 99%

Multisyringe Flow Injection Analysis of Tropomyosin Allergens in Shellfish Samples

et al. 2021

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This paper presents the development and the application of a multisyringe flow injection analysis system for the fluorimetric determination of the major heat-stable known allergen in shrimp, rPen a 1 (tropomyosin). This muscle protein, made up of 284 amino acids, is the main allergen in crustaceans and can be hydrolyzed by microwave in hydrochloric acid medium to produce glutamic acid, the major amino acid in the protein. Glutamic acid can then be quantified specifically by thermal conversion into pyroglutamic acid followed by chemical derivatization of the pyroglutamic acid formed by an analytical protocol based on an OPA-NAC reagent. Pyroglutamic acid can thus be quantified between 1 and 100 µM in less than 15 min with a detection limit of 1.3 µM. The method has been validated by measurements on real samples demonstrating that the response increases with the increase in the tropomyosin content or with the increase in the mass of the shrimp sample.

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Section: Resultsmentioning

confidence: 99%

Multisyringe Flow Injection Analysis of Tropomyosin Allergens in Shellfish Samples

et al. 2021

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“…NH 4 + analysis was carried out by microplate assays following a protocol described in a previous paper based on NH 4 + derivatization with o -phthaldialdehyde (OPA)/ N -acetylcysteine (NAC) . The method LQ was17.07 μg L –1 .…”

Section: Methodsmentioning

confidence: 99%

Underestimation of Anthropogenic Bromoform Released into the Environment?

Quivet

Höhener

Temime-Roussel

et al. 2022

Environ. Sci. Technol.

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Bromoform (CHBr3) belongs to very-short-lived substances (VSLSs), which are important precursors of reactive bromine species (BrOx) contributing to tropospheric and stratospheric chemistry. To date, most models calculating bromine product emissions to the atmosphere only consider the natural production of CHBr3 from marine organisms such as macroalgae and phytoplankton. However, CHBr3 has many other anthropogenic sources (coastal industrial sites, desalination and wastewater plants, ballast waters, and seawater toilets) that may drastically increase the amounts emitted in the atmosphere. Here, we report the levels of CHBr3 released in water and air (according to real-time and offline measurements by proton-transfer-reaction time-of-flight mass spectrometry (PTR-ToF-MS) and gas chromatography with electron capture detection (GC-ECD)) in a highly industrialized area where 3 million cubic meters of chlorinated seawater is released each day, which were 3 measured during six field campaigns (at sea and on land) distributed over three years. The highest levels found during this survey (which were correlated to the physical-chemical characteristics of the water, meteorological and hydrological conditions, salinity, and temperature gradients along the water column) reached 34.6 µg L -1 in water (100-10,000 times higher than reported natural levels) and 3.9 ppbv in the air (100 times higher than the maximum reported value to date). These findings suggest the need to undertake sampling and analysis campaigns as close as possible to chlorinated discharges, as anthropogenic CHBr3 sources from industrial discharges may be a missing factor in global flux estimates or organic bromine to the atmosphere.

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“…The main goal of our work was to develop a new sensitive method for the determination of nitrate by a microplate-based procedure in compost aqueous extracts. In the past, our research group has developed various analytical methods based on the 96-wells technique [17][18][19][20]. In particular, a fluorescence-based microplate procedure for the quantification of ammonium and primary amines in compost water extracts and other matrices was recently assessed [17].…”

Section: Monitoring Of Nitrate In Various Environmental Samples In Thmentioning

confidence: 99%

“…In the past, our research group has developed various analytical methods based on the 96-wells technique [17][18][19][20]. In particular, a fluorescence-based microplate procedure for the quantification of ammonium and primary amines in compost water extracts and other matrices was recently assessed [17]. The basic idea for the assessment of our method was to exploit the previous protocol, reducing first the nitrate to ammonium and then quantifying the latter by means of the method already assessed.…”

Section: Monitoring Of Nitrate In Various Environmental Samples In Thmentioning

confidence: 99%

A highly-sensitive microplate fluorimetric method for the high-throughput determination of nitrate ion in aqueous compost extracts

Ciulu

Ollivier

Demelas

et al. 2018

Microchemical Journal

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In this paper, a new spectrofluorimetric method for the determination of nitrate in aqueous compost extracts is presented. The microplate procedure is based on the reduction of nitrate to ammonium by means of Zn powder under acidic conditions and the following derivatization of ammonium with o-phtaladehyde (OPA) and N-acetylcysteine (NAC) to give a fluorescent derivative. Optimization work allowed performing the reduction in 30 min on a small sample volume (150 µL) and using a little quantity of metal (20 mg). The use of strong acids is also avoided. Excellent limit of detection was achieved (1.3 µM = 0.08 mg.L-1 NO3-) along with good precision levels (4.2 and 14.3 % for 50 and 5 µM, respectively) and a satisfactory linear dynamic range (4-100 µM). A good agreement between data coming from our method and from ion-exchange chromatography was found on real compost samples, indicating a good level of accuracy.

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High throughput determination of ammonium and primary amine compounds in environmental and food samples

Cited by 8 publications

References 23 publications

Multisyringe Flow Injection Analysis of Tropomyosin Allergens in Shellfish Samples

Multisyringe Flow Injection Analysis of Tropomyosin Allergens in Shellfish Samples

Underestimation of Anthropogenic Bromoform Released into the Environment?

A highly-sensitive microplate fluorimetric method for the high-throughput determination of nitrate ion in aqueous compost extracts

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